Amelioration of Murine Colitis by Attenuated <i>Salmonella choleraesuis</i> Encoding Interleukin-19
The imbalance of mucosal immunity in the lower gastrointestinal tract can lead to chronic inflammatory bowel diseases (IBDs), including Crohn’s disease and ulcerative colitis. IBD is a chronic inflammatory disorder that causes small and/or large intestines ulceration. According to previous studies,...
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MDPI AG
2023-06-01
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| Series: | Microorganisms |
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| author | Shih-Yao Chen Chun-Ting Chu Mei-Lin Yang Jian-Da Lin Chung-Teng Wang Che-Hsin Lee I-Chen Lin Ai-Li Shiau Pin Ling Chao-Liang Wu |
| author_facet | Shih-Yao Chen Chun-Ting Chu Mei-Lin Yang Jian-Da Lin Chung-Teng Wang Che-Hsin Lee I-Chen Lin Ai-Li Shiau Pin Ling Chao-Liang Wu |
| author_sort | Shih-Yao Chen |
| collection | DOAJ |
| description | The imbalance of mucosal immunity in the lower gastrointestinal tract can lead to chronic inflammatory bowel diseases (IBDs), including Crohn’s disease and ulcerative colitis. IBD is a chronic inflammatory disorder that causes small and/or large intestines ulceration. According to previous studies, recombinant interleukin (IL)-10 protein and genetically modified bacteria secreting IL-10 ameliorate dextran sulfate sodium (DSS)-induced colitis in mice. IL-19 is a transcriptional activator of IL-10 and can alter the balance of T helper 1 (Th)1/Th2 cells in favor of Th2. In this study, we aimed to investigate whether the expression of the murine IL-19 gene carried by <i>Salmonella choleraesuis</i> (<i>S. choleraesuis</i>) could ameliorate murine IBD. Our results showed that the attenuated <i>S. choleraesuis</i> could carry and express the IL-19 gene-containing plasmid for IBD gene therapy by reducing the mortality and clinical signs in DSS-induced acute colitis mice as compared to the untreated ones. We also found that IL-10 expression was induced in IL-19-treated colitis mice and prevented inflammatory infiltrates and proinflammatory cytokine expression in these mice. We suggest that <i>S. choleraesuis</i> encoding IL-19 provides a new strategy for treating IBD in the future. |
| first_indexed | 2024-03-11T02:08:02Z |
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| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-11T02:08:02Z |
| publishDate | 2023-06-01 |
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| series | Microorganisms |
| spelling | doaj.art-272d0b8add514772b585d2d379284eb02023-11-18T11:43:10ZengMDPI AGMicroorganisms2076-26072023-06-01116153010.3390/microorganisms11061530Amelioration of Murine Colitis by Attenuated <i>Salmonella choleraesuis</i> Encoding Interleukin-19Shih-Yao Chen0Chun-Ting Chu1Mei-Lin Yang2Jian-Da Lin3Chung-Teng Wang4Che-Hsin Lee5I-Chen Lin6Ai-Li Shiau7Pin Ling8Chao-Liang Wu9Department of Nursing, College of Nursing, Chung Hwa University of Medical Technology, Tainan 71703, TaiwanDivision of Colorectal Surgery, Department of Surgery, Ditmanson Medical Foundation Chia-Yi Christian Hospital, 539, Zhongxiao Road, Chiayi City 60002, TaiwanDitmanson Medical Foundation Chia-Yi Christian Hospital, Chiayi City 60002, TaiwanDepartment of Microbiology and Immunology, College of Medicine, National Cheng Kung University, Tainan 70101, TaiwanDepartment of Microbiology and Immunology, College of Medicine, National Cheng Kung University, Tainan 70101, TaiwanDepartment of Biological Sciences, National Sun Yat-sen University, Kaohsiung 80424, TaiwanDivision of Colorectal Surgery, Department of Surgery, Ditmanson Medical Foundation Chia-Yi Christian Hospital, 539, Zhongxiao Road, Chiayi City 60002, TaiwanDitmanson Medical Foundation Chia-Yi Christian Hospital, Chiayi City 60002, TaiwanDepartment of Microbiology and Immunology, College of Medicine, National Cheng Kung University, Tainan 70101, TaiwanDitmanson Medical Foundation Chia-Yi Christian Hospital, Chiayi City 60002, TaiwanThe imbalance of mucosal immunity in the lower gastrointestinal tract can lead to chronic inflammatory bowel diseases (IBDs), including Crohn’s disease and ulcerative colitis. IBD is a chronic inflammatory disorder that causes small and/or large intestines ulceration. According to previous studies, recombinant interleukin (IL)-10 protein and genetically modified bacteria secreting IL-10 ameliorate dextran sulfate sodium (DSS)-induced colitis in mice. IL-19 is a transcriptional activator of IL-10 and can alter the balance of T helper 1 (Th)1/Th2 cells in favor of Th2. In this study, we aimed to investigate whether the expression of the murine IL-19 gene carried by <i>Salmonella choleraesuis</i> (<i>S. choleraesuis</i>) could ameliorate murine IBD. Our results showed that the attenuated <i>S. choleraesuis</i> could carry and express the IL-19 gene-containing plasmid for IBD gene therapy by reducing the mortality and clinical signs in DSS-induced acute colitis mice as compared to the untreated ones. We also found that IL-10 expression was induced in IL-19-treated colitis mice and prevented inflammatory infiltrates and proinflammatory cytokine expression in these mice. We suggest that <i>S. choleraesuis</i> encoding IL-19 provides a new strategy for treating IBD in the future.https://www.mdpi.com/2076-2607/11/6/1530inflammatory bowel diseasesinterleukin-19<i>Salmonella choleraesuis</i>dextran sulfate sodium-induced colitis |
| spellingShingle | Shih-Yao Chen Chun-Ting Chu Mei-Lin Yang Jian-Da Lin Chung-Teng Wang Che-Hsin Lee I-Chen Lin Ai-Li Shiau Pin Ling Chao-Liang Wu Amelioration of Murine Colitis by Attenuated <i>Salmonella choleraesuis</i> Encoding Interleukin-19 Microorganisms inflammatory bowel diseases interleukin-19 <i>Salmonella choleraesuis</i> dextran sulfate sodium-induced colitis |
| title | Amelioration of Murine Colitis by Attenuated <i>Salmonella choleraesuis</i> Encoding Interleukin-19 |
| title_full | Amelioration of Murine Colitis by Attenuated <i>Salmonella choleraesuis</i> Encoding Interleukin-19 |
| title_fullStr | Amelioration of Murine Colitis by Attenuated <i>Salmonella choleraesuis</i> Encoding Interleukin-19 |
| title_full_unstemmed | Amelioration of Murine Colitis by Attenuated <i>Salmonella choleraesuis</i> Encoding Interleukin-19 |
| title_short | Amelioration of Murine Colitis by Attenuated <i>Salmonella choleraesuis</i> Encoding Interleukin-19 |
| title_sort | amelioration of murine colitis by attenuated i salmonella choleraesuis i encoding interleukin 19 |
| topic | inflammatory bowel diseases interleukin-19 <i>Salmonella choleraesuis</i> dextran sulfate sodium-induced colitis |
| url | https://www.mdpi.com/2076-2607/11/6/1530 |
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