Alkaloid-rich extract of Lannea egregia leaf protect against cardiomyopathy in streptozotocin-induced type 2 diabetic rats
Background: Lannea egregia leaf has been used locally for the management of diseases with limited scientific support. Hence, the cardiomyopathy protective ability of an alkaloid-rich extract of L. egregia leaf in streptozotocin-induced type 2 diabetic rats was investigated in this study. A L. egregi...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-02-01
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| Series: | Phytomedicine Plus |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667031323001094 |
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| author | B.O. Ajiboye I.M. Folorunso K.I. Akinfemiwa B.E. Oyinloye O.E. Lawal O.A. Ojo M. Ezema O.R. Ajuwon E.A. Ardekani |
| author_facet | B.O. Ajiboye I.M. Folorunso K.I. Akinfemiwa B.E. Oyinloye O.E. Lawal O.A. Ojo M. Ezema O.R. Ajuwon E.A. Ardekani |
| author_sort | B.O. Ajiboye |
| collection | DOAJ |
| description | Background: Lannea egregia leaf has been used locally for the management of diseases with limited scientific support. Hence, the cardiomyopathy protective ability of an alkaloid-rich extract of L. egregia leaf in streptozotocin-induced type 2 diabetic rats was investigated in this study. A L. egregia leaf alkaloid-rich extract was obtained using a standard procedure. Streptozotocin was injected into the experimental animals intraperitoneal at a dose of 45 mg/kg body weight to induce type 2 diabetes mellitus, prior to this, the animals were given 20 % (w/v) fructose for one week. Thus, the animals were grouped into five (n = 8), comprising normal control (NC), diabetic control (DC), diabetic rats treated with low (50 mg/kg body weight) and high (100 mg/kg body weight) doses of L. egregia alkaloid-rich leaf extracts (i.e., LEL and LEH, respectively), and 200 mg/kg body weight metformin (MET). The animals were sacrificed on the 21st day, and blood and hearts were harvested and used for the determination of serum lipid profiles, atherogenic indices, oxidative stress biomarkers, ATPase activities, cardiac enzyme activities, creatinine kinase-MB, cardiac troponin, cytokines, N-terminal pro-B-type brain natriuretic peptide (NT-pro BNP) and histology examination. Results: The results revealed that diabetic rats treated with LEL, LEH, and MET significantly (p < 0.05) reduced their fasting blood glucose levels, improved their serum lipid profile, and decreased their cardio-risk ratio, atherogenic index of plasma, atherogenic coefficient, and atherosclerosis index. In addition, diabetic treated with on LEL, LEH, and MET significantly (p < 0.05) ameliorated oxidative stress biomarkers, increased selected ATPase activities, increased specific cardiac enzyme activities, and decreased serum creatine kinase-MB, serum cardiac troponin I and T, as well as cytokines and NT-pro BNP in diabetic rats. These were supported by the histology examination by improving myocardial degeneration in diabetic rats administered LEL, LEH, and MET. Conclusion: Hence, it can be presumed that alkaloid-rich extracts obtained from L. egregia leaves could be beneficial in ameliorating diabetic cardiomyopathy conditions. |
| first_indexed | 2024-03-08T05:54:20Z |
| format | Article |
| id | doaj.art-272d762f133c4cc1a53531b93a209e76 |
| institution | Directory Open Access Journal |
| issn | 2667-0313 |
| language | English |
| last_indexed | 2024-03-08T05:54:20Z |
| publishDate | 2024-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Phytomedicine Plus |
| spelling | doaj.art-272d762f133c4cc1a53531b93a209e762024-02-05T04:32:22ZengElsevierPhytomedicine Plus2667-03132024-02-0141100513Alkaloid-rich extract of Lannea egregia leaf protect against cardiomyopathy in streptozotocin-induced type 2 diabetic ratsB.O. Ajiboye0I.M. Folorunso1K.I. Akinfemiwa2B.E. Oyinloye3O.E. Lawal4O.A. Ojo5M. Ezema6O.R. Ajuwon7E.A. Ardekani8Phytomedicine and Molecular Toxicology Research Laboratory, Department of Biochemistry, Federal University Oye-Ekiti, Ekiti State, Nigeria; Institute of Drug Research and Development, SE Bogoro Center, Afe Babalola University, Ado-Ekiti, Nigeria; Corresponding authors.Department of Biochemistry, Federal University of Technology Akure, NigeriaPhytomedicine and Molecular Toxicology Research Laboratory, Department of Biochemistry, Federal University Oye-Ekiti, Ekiti State, NigeriaInstitute of Drug Research and Development, SE Bogoro Center, Afe Babalola University, Ado-Ekiti, Nigeria; Phytomedicine, Biochemical Toxicology and Biotechnology Research Laboratories, Department of Biochemistry, College of Sciences, Afe Babalola University, PMB 5454, Ado-Ekiti, 360001, Nigeria; Biotechnology and Structural Biology (BSB) Group, Department of Biochemistry and Microbiology, University of Zululand, KwaDlangezwa, 3886, South AfricaPhytomedicine and Molecular Toxicology Research Laboratory, Department of Biochemistry, Federal University Oye-Ekiti, Ekiti State, NigeriaPhytomedicine, Molecular Toxicology, and Computational Biochemistry Research Laboratory (PMTCB-RL), Department of Biochemistry, Bowen University, Iwo, 232101, Osun State, Nigeria; Corresponding authors.Functional Foods and Phytomedicine Research Group, Department of Biochemistry, Federal University Oye-Ekiti, PMB 373, Oye-Ekiti, Ekiti State, NigeriaRedox Biology Research Laboratory, Department of Biochemistry, Federal University Oye-Ekiti, Ekiti State, NigeriaPharmaceutical Sciences, and Functional Foods, Shiraz University of Medical Sciences, IranBackground: Lannea egregia leaf has been used locally for the management of diseases with limited scientific support. Hence, the cardiomyopathy protective ability of an alkaloid-rich extract of L. egregia leaf in streptozotocin-induced type 2 diabetic rats was investigated in this study. A L. egregia leaf alkaloid-rich extract was obtained using a standard procedure. Streptozotocin was injected into the experimental animals intraperitoneal at a dose of 45 mg/kg body weight to induce type 2 diabetes mellitus, prior to this, the animals were given 20 % (w/v) fructose for one week. Thus, the animals were grouped into five (n = 8), comprising normal control (NC), diabetic control (DC), diabetic rats treated with low (50 mg/kg body weight) and high (100 mg/kg body weight) doses of L. egregia alkaloid-rich leaf extracts (i.e., LEL and LEH, respectively), and 200 mg/kg body weight metformin (MET). The animals were sacrificed on the 21st day, and blood and hearts were harvested and used for the determination of serum lipid profiles, atherogenic indices, oxidative stress biomarkers, ATPase activities, cardiac enzyme activities, creatinine kinase-MB, cardiac troponin, cytokines, N-terminal pro-B-type brain natriuretic peptide (NT-pro BNP) and histology examination. Results: The results revealed that diabetic rats treated with LEL, LEH, and MET significantly (p < 0.05) reduced their fasting blood glucose levels, improved their serum lipid profile, and decreased their cardio-risk ratio, atherogenic index of plasma, atherogenic coefficient, and atherosclerosis index. In addition, diabetic treated with on LEL, LEH, and MET significantly (p < 0.05) ameliorated oxidative stress biomarkers, increased selected ATPase activities, increased specific cardiac enzyme activities, and decreased serum creatine kinase-MB, serum cardiac troponin I and T, as well as cytokines and NT-pro BNP in diabetic rats. These were supported by the histology examination by improving myocardial degeneration in diabetic rats administered LEL, LEH, and MET. Conclusion: Hence, it can be presumed that alkaloid-rich extracts obtained from L. egregia leaves could be beneficial in ameliorating diabetic cardiomyopathy conditions.http://www.sciencedirect.com/science/article/pii/S2667031323001094AlkaloidLannea egregiaDiabetes mellitusAtherogenicOxidative stress |
| spellingShingle | B.O. Ajiboye I.M. Folorunso K.I. Akinfemiwa B.E. Oyinloye O.E. Lawal O.A. Ojo M. Ezema O.R. Ajuwon E.A. Ardekani Alkaloid-rich extract of Lannea egregia leaf protect against cardiomyopathy in streptozotocin-induced type 2 diabetic rats Phytomedicine Plus Alkaloid Lannea egregia Diabetes mellitus Atherogenic Oxidative stress |
| title | Alkaloid-rich extract of Lannea egregia leaf protect against cardiomyopathy in streptozotocin-induced type 2 diabetic rats |
| title_full | Alkaloid-rich extract of Lannea egregia leaf protect against cardiomyopathy in streptozotocin-induced type 2 diabetic rats |
| title_fullStr | Alkaloid-rich extract of Lannea egregia leaf protect against cardiomyopathy in streptozotocin-induced type 2 diabetic rats |
| title_full_unstemmed | Alkaloid-rich extract of Lannea egregia leaf protect against cardiomyopathy in streptozotocin-induced type 2 diabetic rats |
| title_short | Alkaloid-rich extract of Lannea egregia leaf protect against cardiomyopathy in streptozotocin-induced type 2 diabetic rats |
| title_sort | alkaloid rich extract of lannea egregia leaf protect against cardiomyopathy in streptozotocin induced type 2 diabetic rats |
| topic | Alkaloid Lannea egregia Diabetes mellitus Atherogenic Oxidative stress |
| url | http://www.sciencedirect.com/science/article/pii/S2667031323001094 |
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