A mixture of St. John's wort and sea buckthorn oils regresses endometriotic implants and affects the levels of inflammatory mediators in peritoneal fluid of the rat: A surgically induced endometriosis model
Objective: Sea buckthorn (Hippophae rhamnoides L.) and St. John's wort (Hypericum perforatum L.) are used as an emmenagog and for the treatment of other gynecological disorders including uterus inflammation and endometriosis. The aim of the present study is to investigate the potential of a mix...
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| Format: | Article |
| Language: | English |
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Elsevier
2016-12-01
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| Series: | Taiwanese Journal of Obstetrics & Gynecology |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1028455916301875 |
| _version_ | 1828476350388764672 |
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| author | Mert İlhan İpek Süntar Mürşide Ayşe Demirel Erdem Yeşilada Hikmet Keleş Esra Küpeli Akkol |
| author_facet | Mert İlhan İpek Süntar Mürşide Ayşe Demirel Erdem Yeşilada Hikmet Keleş Esra Küpeli Akkol |
| author_sort | Mert İlhan |
| collection | DOAJ |
| description | Objective: Sea buckthorn (Hippophae rhamnoides L.) and St. John's wort (Hypericum perforatum L.) are used as an emmenagog and for the treatment of other gynecological disorders including uterus inflammation and endometriosis. The aim of the present study is to investigate the potential of a mixture of sea buckthorn and St. John's wort oils (HrHp oil) in the treatment of endometriosis.
Materials and Methods: The activity was assessed in surgically induced endometriosis in rats. A 15-mm piece of endometrium was sutured into the abdominal wall. Twenty-eight days later, a second laparotomy was performed to calculate the endometrial foci areas and to score intra-abdominal adhesions. The rats were treated with either vehicle, HrHp oil formulation, or the reference (buserelin acetate). At the end of the experiment all rats were sacrificed and endometriotic foci areas and intra-abdominal adhesions were re-evaluated. The tissue sections were analyzed histopathologically. Peritoneal fluids of the experimental animals were collected in order to detect the levels of tumor necrosis factor-α, vascular endothelial growth factor, and interleukin-6, which might be involved in the etiology of endometriosis.
Results: In the HrHp oil-treated group, the volumes of endometriotic implants were found to be significantly decreased (from 50.8 mm3 to 18.6 mm3, p < 0.001) without any adhesion (0.0 ± 0.0, p < 0.001) when compared to the control group (3.1 ± 0.9). The levels of tumor necrosis factor-α decreased from 7.02 ± 1.33 pg/mL to 4.78 ± 1.02 pg/mL (p < 0.01); vascular endothelial growth factor from 17.39 ± 8.52 pg/mL to 9.67 ± 5.04 pg/mL (p < 0.01); and interleukin-6 from 50.95 ± 22.84 pg/mL to 29.11 ± 7.45 pg/mL (p < 0.01), respectively, after HrHp oil treatment.
Conclusion: HrHp oil may be a promising alternative for the treatment of endometriosis. |
| first_indexed | 2024-12-11T06:27:33Z |
| format | Article |
| id | doaj.art-272dab0505ef4e1c83df542e27360f38 |
| institution | Directory Open Access Journal |
| issn | 1028-4559 |
| language | English |
| last_indexed | 2024-12-11T06:27:33Z |
| publishDate | 2016-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Taiwanese Journal of Obstetrics & Gynecology |
| spelling | doaj.art-272dab0505ef4e1c83df542e27360f382022-12-22T01:17:37ZengElsevierTaiwanese Journal of Obstetrics & Gynecology1028-45592016-12-0155678679010.1016/j.tjog.2015.01.006A mixture of St. John's wort and sea buckthorn oils regresses endometriotic implants and affects the levels of inflammatory mediators in peritoneal fluid of the rat: A surgically induced endometriosis modelMert İlhan0İpek Süntar1Mürşide Ayşe Demirel2Erdem Yeşilada3Hikmet Keleş4Esra Küpeli Akkol5Department of Pharmacognosy, Faculty of Pharmacy, Gazi University, Etiler, Ankara, TurkeyDepartment of Pharmacognosy, Faculty of Pharmacy, Gazi University, Etiler, Ankara, TurkeyLaboratory Animals Breeding and Experimental Researches Center, Faculty of Pharmacy, Gazi University, Etiler, Ankara, TurkeyDepartment of Pharmacognosy, Faculty of Pharmacy, Yeditepe University, Atasehir, Istanbul, TurkeyDepartment of Pathology, Faculty of Veterinary Medicine, Afyon Kocatepe University, Afyonkarahisar, TurkeyDepartment of Pharmacognosy, Faculty of Pharmacy, Gazi University, Etiler, Ankara, TurkeyObjective: Sea buckthorn (Hippophae rhamnoides L.) and St. John's wort (Hypericum perforatum L.) are used as an emmenagog and for the treatment of other gynecological disorders including uterus inflammation and endometriosis. The aim of the present study is to investigate the potential of a mixture of sea buckthorn and St. John's wort oils (HrHp oil) in the treatment of endometriosis. Materials and Methods: The activity was assessed in surgically induced endometriosis in rats. A 15-mm piece of endometrium was sutured into the abdominal wall. Twenty-eight days later, a second laparotomy was performed to calculate the endometrial foci areas and to score intra-abdominal adhesions. The rats were treated with either vehicle, HrHp oil formulation, or the reference (buserelin acetate). At the end of the experiment all rats were sacrificed and endometriotic foci areas and intra-abdominal adhesions were re-evaluated. The tissue sections were analyzed histopathologically. Peritoneal fluids of the experimental animals were collected in order to detect the levels of tumor necrosis factor-α, vascular endothelial growth factor, and interleukin-6, which might be involved in the etiology of endometriosis. Results: In the HrHp oil-treated group, the volumes of endometriotic implants were found to be significantly decreased (from 50.8 mm3 to 18.6 mm3, p < 0.001) without any adhesion (0.0 ± 0.0, p < 0.001) when compared to the control group (3.1 ± 0.9). The levels of tumor necrosis factor-α decreased from 7.02 ± 1.33 pg/mL to 4.78 ± 1.02 pg/mL (p < 0.01); vascular endothelial growth factor from 17.39 ± 8.52 pg/mL to 9.67 ± 5.04 pg/mL (p < 0.01); and interleukin-6 from 50.95 ± 22.84 pg/mL to 29.11 ± 7.45 pg/mL (p < 0.01), respectively, after HrHp oil treatment. Conclusion: HrHp oil may be a promising alternative for the treatment of endometriosis.http://www.sciencedirect.com/science/article/pii/S1028455916301875cytokineexperimental endometriosis modelHippophae rhamnoides (sea buckthorn)Hypericum perforatum (St. John's wort) |
| spellingShingle | Mert İlhan İpek Süntar Mürşide Ayşe Demirel Erdem Yeşilada Hikmet Keleş Esra Küpeli Akkol A mixture of St. John's wort and sea buckthorn oils regresses endometriotic implants and affects the levels of inflammatory mediators in peritoneal fluid of the rat: A surgically induced endometriosis model Taiwanese Journal of Obstetrics & Gynecology cytokine experimental endometriosis model Hippophae rhamnoides (sea buckthorn) Hypericum perforatum (St. John's wort) |
| title | A mixture of St. John's wort and sea buckthorn oils regresses endometriotic implants and affects the levels of inflammatory mediators in peritoneal fluid of the rat: A surgically induced endometriosis model |
| title_full | A mixture of St. John's wort and sea buckthorn oils regresses endometriotic implants and affects the levels of inflammatory mediators in peritoneal fluid of the rat: A surgically induced endometriosis model |
| title_fullStr | A mixture of St. John's wort and sea buckthorn oils regresses endometriotic implants and affects the levels of inflammatory mediators in peritoneal fluid of the rat: A surgically induced endometriosis model |
| title_full_unstemmed | A mixture of St. John's wort and sea buckthorn oils regresses endometriotic implants and affects the levels of inflammatory mediators in peritoneal fluid of the rat: A surgically induced endometriosis model |
| title_short | A mixture of St. John's wort and sea buckthorn oils regresses endometriotic implants and affects the levels of inflammatory mediators in peritoneal fluid of the rat: A surgically induced endometriosis model |
| title_sort | mixture of st john s wort and sea buckthorn oils regresses endometriotic implants and affects the levels of inflammatory mediators in peritoneal fluid of the rat a surgically induced endometriosis model |
| topic | cytokine experimental endometriosis model Hippophae rhamnoides (sea buckthorn) Hypericum perforatum (St. John's wort) |
| url | http://www.sciencedirect.com/science/article/pii/S1028455916301875 |
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