Enterobacter sp. DBA51 produces ACC deaminase and promotes the growth of tomato (Solanum lycopersicum L.) and tobacco (Nicotiana tabacum L.) plants under greenhouse condition
Bacterial isolated from rhizospheric soil associated with the semi-desertic plant Coronilla juncea L. were screened for 1-aminocyclopropane-1-carboxylate deaminase (ACCD) activity, a common trait for plant-growth-promoting rhizobacteria (PGPR). Among bacterial isolates, strain DBA51 showed phosphate...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-01-01
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| Series: | Current Research in Microbial Sciences |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666517423000287 |
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| author | Yolanda Ortega-Ortega Luis Gerardo Sarmiento-López Adolfo Baylón-Palomino Jairo Vázquez-Lee Luis David Maldonado-Bonilla Alberto Flores-Olivas José Humberto Valenzuela-Soto |
| author_facet | Yolanda Ortega-Ortega Luis Gerardo Sarmiento-López Adolfo Baylón-Palomino Jairo Vázquez-Lee Luis David Maldonado-Bonilla Alberto Flores-Olivas José Humberto Valenzuela-Soto |
| author_sort | Yolanda Ortega-Ortega |
| collection | DOAJ |
| description | Bacterial isolated from rhizospheric soil associated with the semi-desertic plant Coronilla juncea L. were screened for 1-aminocyclopropane-1-carboxylate deaminase (ACCD) activity, a common trait for plant-growth-promoting rhizobacteria (PGPR). Among bacterial isolates, strain DBA51 showed phosphate solubilizing index (PSI), producing indole acetic acid (IAA), and with the hemolysis-negative test. Sequencing and analysis of the 16S rDNA gene identified DBA51 as Enterobacter. DBA51 did not show antagonistic activity in vitro against bacterial (Clavibacter michiganensis, Pseudomonas syringae pv. tomato DC3000 and Pectobacterium cacticidum FHLGJ22) and fungal phytopathogens (Alternaria sp., Fusarium oxysporum fsp. lycopersici, Fusarium oxysporum fsp. cubense M5, and Rhizoctonia sp.). Root inoculations with DBA51 in tomato (Solanum lycopersicum L.) and tobacco (Nicotiana tabacum L.) plants were performed under greenhouse conditions. Plant height (20 %) and root biomass (40 %) were significantly enhanced in tomato plants inoculated with DBA51 compared to non-inoculated plants, although for tobacco plants, only root biomass (27 %) showed significant differences with DBA51. In addition, physiological parameters such as photosynthetic rate (µmol CO2 m−2 s-1), stomatal conductance (mol H2O m−2 s−1), and transpiration rate (mmol H2O m−2 s−1) were also evaluated, and no differences were detected between DBA51-inoculated and control treatment in tomato and tobacco leaves. The observed results indicate that the DBA51 strain could be used as a biofertilizer to improve yields of horticultural crops. |
| first_indexed | 2024-03-08T22:55:13Z |
| format | Article |
| id | doaj.art-272ea822db80474faf06a4e511c0baad |
| institution | Directory Open Access Journal |
| issn | 2666-5174 |
| language | English |
| last_indexed | 2024-03-08T22:55:13Z |
| publishDate | 2024-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Current Research in Microbial Sciences |
| spelling | doaj.art-272ea822db80474faf06a4e511c0baad2023-12-16T06:09:17ZengElsevierCurrent Research in Microbial Sciences2666-51742024-01-016100207Enterobacter sp. DBA51 produces ACC deaminase and promotes the growth of tomato (Solanum lycopersicum L.) and tobacco (Nicotiana tabacum L.) plants under greenhouse conditionYolanda Ortega-Ortega0Luis Gerardo Sarmiento-López1Adolfo Baylón-Palomino2Jairo Vázquez-Lee3Luis David Maldonado-Bonilla4Alberto Flores-Olivas5José Humberto Valenzuela-Soto6Centro de Investigación en Química Aplicada, Departamento de Biociencias y Agrotecnología, Blvd. Enrique Reyna 104, 25294, Saltillo, Coahuila, MexicoCentro de Investigación en Química Aplicada, Departamento de Biociencias y Agrotecnología, Blvd. Enrique Reyna 104, 25294, Saltillo, Coahuila, MexicoCentro de Investigación en Química Aplicada, Departamento de Biociencias y Agrotecnología, Blvd. Enrique Reyna 104, 25294, Saltillo, Coahuila, MexicoCentro de Investigación en Química Aplicada, Departamento de Biociencias y Agrotecnología, Blvd. Enrique Reyna 104, 25294, Saltillo, Coahuila, MexicoCONAHCYT-Universidad del Mar campus Puerto Escondido, Instituto de Genética, Puerto Escondido, Oaxaca, MexicoUniversidad Autónoma Agraria Antonio Narro, Departamento de Parasitología, Buenavista, 25315, Saltillo, Coahuila, MexicoCentro de Investigación en Química Aplicada, Departamento de Biociencias y Agrotecnología, Blvd. Enrique Reyna 104, 25294, Saltillo, Coahuila, Mexico; CONAHCYT-Centro de Investigación en Química Aplicada, Blvd. Enrique Reyna 104, 25294, Saltillo, Coahuila, Mexico; Corresponding author.Bacterial isolated from rhizospheric soil associated with the semi-desertic plant Coronilla juncea L. were screened for 1-aminocyclopropane-1-carboxylate deaminase (ACCD) activity, a common trait for plant-growth-promoting rhizobacteria (PGPR). Among bacterial isolates, strain DBA51 showed phosphate solubilizing index (PSI), producing indole acetic acid (IAA), and with the hemolysis-negative test. Sequencing and analysis of the 16S rDNA gene identified DBA51 as Enterobacter. DBA51 did not show antagonistic activity in vitro against bacterial (Clavibacter michiganensis, Pseudomonas syringae pv. tomato DC3000 and Pectobacterium cacticidum FHLGJ22) and fungal phytopathogens (Alternaria sp., Fusarium oxysporum fsp. lycopersici, Fusarium oxysporum fsp. cubense M5, and Rhizoctonia sp.). Root inoculations with DBA51 in tomato (Solanum lycopersicum L.) and tobacco (Nicotiana tabacum L.) plants were performed under greenhouse conditions. Plant height (20 %) and root biomass (40 %) were significantly enhanced in tomato plants inoculated with DBA51 compared to non-inoculated plants, although for tobacco plants, only root biomass (27 %) showed significant differences with DBA51. In addition, physiological parameters such as photosynthetic rate (µmol CO2 m−2 s-1), stomatal conductance (mol H2O m−2 s−1), and transpiration rate (mmol H2O m−2 s−1) were also evaluated, and no differences were detected between DBA51-inoculated and control treatment in tomato and tobacco leaves. The observed results indicate that the DBA51 strain could be used as a biofertilizer to improve yields of horticultural crops.http://www.sciencedirect.com/science/article/pii/S2666517423000287Rhizobacteria1-aminocyclopropane-1-carboxylatePhosphate solubilizationEnterobacterGrowth promotion |
| spellingShingle | Yolanda Ortega-Ortega Luis Gerardo Sarmiento-López Adolfo Baylón-Palomino Jairo Vázquez-Lee Luis David Maldonado-Bonilla Alberto Flores-Olivas José Humberto Valenzuela-Soto Enterobacter sp. DBA51 produces ACC deaminase and promotes the growth of tomato (Solanum lycopersicum L.) and tobacco (Nicotiana tabacum L.) plants under greenhouse condition Current Research in Microbial Sciences Rhizobacteria 1-aminocyclopropane-1-carboxylate Phosphate solubilization Enterobacter Growth promotion |
| title | Enterobacter sp. DBA51 produces ACC deaminase and promotes the growth of tomato (Solanum lycopersicum L.) and tobacco (Nicotiana tabacum L.) plants under greenhouse condition |
| title_full | Enterobacter sp. DBA51 produces ACC deaminase and promotes the growth of tomato (Solanum lycopersicum L.) and tobacco (Nicotiana tabacum L.) plants under greenhouse condition |
| title_fullStr | Enterobacter sp. DBA51 produces ACC deaminase and promotes the growth of tomato (Solanum lycopersicum L.) and tobacco (Nicotiana tabacum L.) plants under greenhouse condition |
| title_full_unstemmed | Enterobacter sp. DBA51 produces ACC deaminase and promotes the growth of tomato (Solanum lycopersicum L.) and tobacco (Nicotiana tabacum L.) plants under greenhouse condition |
| title_short | Enterobacter sp. DBA51 produces ACC deaminase and promotes the growth of tomato (Solanum lycopersicum L.) and tobacco (Nicotiana tabacum L.) plants under greenhouse condition |
| title_sort | enterobacter sp dba51 produces acc deaminase and promotes the growth of tomato solanum lycopersicum l and tobacco nicotiana tabacum l plants under greenhouse condition |
| topic | Rhizobacteria 1-aminocyclopropane-1-carboxylate Phosphate solubilization Enterobacter Growth promotion |
| url | http://www.sciencedirect.com/science/article/pii/S2666517423000287 |
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