Comparative transcriptome analysis of differentially expressed genes related to the physiological changes of yellow-green leaf mutant of maize
Chlorophylls, green pigments in chloroplasts, are essential for photosynthesis. Reduction in chlorophyll content may result in retarded growth, dwarfism, and sterility. In this study, a yellow-green leaf mutant of maize, indicative of abnormity in chlorophyll content, was identified. The physiologic...
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PeerJ Inc.
2021-02-01
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| author | Tingchun Li Huaying Yang Yan Lu Qing Dong Guihu Liu Feng Chen Yingbing Zhou |
| author_facet | Tingchun Li Huaying Yang Yan Lu Qing Dong Guihu Liu Feng Chen Yingbing Zhou |
| author_sort | Tingchun Li |
| collection | DOAJ |
| description | Chlorophylls, green pigments in chloroplasts, are essential for photosynthesis. Reduction in chlorophyll content may result in retarded growth, dwarfism, and sterility. In this study, a yellow-green leaf mutant of maize, indicative of abnormity in chlorophyll content, was identified. The physiological parameters of this mutant were measured. Next, global gene expression of this mutant was determined using transcriptome analysis and compared to that of wild-type maize plants. The yellow-green leaf mutant of maize was found to contain lower contents of chlorophyll a, chlorophyll b and carotenoid compounds. It contained fewer active PSII centers and displayed lower values of original chlorophyll fluorescence parameters than the wild-type plants. The real-time fluorescence yield, the electron transport rate, and the net photosynthetic rate of the mutant plants showed reduction as well. In contrast, the maximum photochemical quantum yield of PSII of the mutant plants was similar to that of the wild-type plants. Comparative transcriptome analysis of the mutant plants and wild-type plants led to the identification of differentially expressed 1,122 genes, of which 536 genes were up-regulated and 586 genes down-regulated in the mutant. Five genes in the chlorophyll metabolism pathway, nine genes in the tricarboxylic acid cycle and seven genes related to the conversion of sucrose to starch displayed down-regulated expression. In contrast, genes encoding a photosystem II reaction center PsbP family protein and the PGR5-like protein 1A (PGRL1A) exhibited increased transcript abundance. |
| first_indexed | 2024-03-09T06:22:33Z |
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| language | English |
| last_indexed | 2024-03-09T06:22:33Z |
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| spelling | doaj.art-2730150994ae480e8bf0b86da1f682882023-12-03T11:32:46ZengPeerJ Inc.PeerJ2167-83592021-02-019e1056710.7717/peerj.10567Comparative transcriptome analysis of differentially expressed genes related to the physiological changes of yellow-green leaf mutant of maizeTingchun Li0Huaying Yang1Yan Lu2Qing Dong3Guihu Liu4Feng Chen5Yingbing Zhou6Tobacco Research Institute, Anhui Academy of Agricultural Sciences, Hefei, ChinaTobacco Research Institute, Anhui Academy of Agricultural Sciences, Hefei, ChinaDepartment of Biological Sciences, Western Michigan University, Kalamazoo, MI, USATobacco Research Institute, Anhui Academy of Agricultural Sciences, Hefei, ChinaTobacco Research Institute, Anhui Academy of Agricultural Sciences, Hefei, ChinaDepartment of Plant Sciences, University of Tennessee, Knoxville, TN, USATobacco Research Institute, Anhui Academy of Agricultural Sciences, Hefei, ChinaChlorophylls, green pigments in chloroplasts, are essential for photosynthesis. Reduction in chlorophyll content may result in retarded growth, dwarfism, and sterility. In this study, a yellow-green leaf mutant of maize, indicative of abnormity in chlorophyll content, was identified. The physiological parameters of this mutant were measured. Next, global gene expression of this mutant was determined using transcriptome analysis and compared to that of wild-type maize plants. The yellow-green leaf mutant of maize was found to contain lower contents of chlorophyll a, chlorophyll b and carotenoid compounds. It contained fewer active PSII centers and displayed lower values of original chlorophyll fluorescence parameters than the wild-type plants. The real-time fluorescence yield, the electron transport rate, and the net photosynthetic rate of the mutant plants showed reduction as well. In contrast, the maximum photochemical quantum yield of PSII of the mutant plants was similar to that of the wild-type plants. Comparative transcriptome analysis of the mutant plants and wild-type plants led to the identification of differentially expressed 1,122 genes, of which 536 genes were up-regulated and 586 genes down-regulated in the mutant. Five genes in the chlorophyll metabolism pathway, nine genes in the tricarboxylic acid cycle and seven genes related to the conversion of sucrose to starch displayed down-regulated expression. In contrast, genes encoding a photosystem II reaction center PsbP family protein and the PGR5-like protein 1A (PGRL1A) exhibited increased transcript abundance.https://peerj.com/articles/10567.pdfYellow-green leafChlorophyll biosynthesisPhotosynthesisTricarboxylic acid cycleSecondary metabolismTranscriptome analysis |
| spellingShingle | Tingchun Li Huaying Yang Yan Lu Qing Dong Guihu Liu Feng Chen Yingbing Zhou Comparative transcriptome analysis of differentially expressed genes related to the physiological changes of yellow-green leaf mutant of maize PeerJ Yellow-green leaf Chlorophyll biosynthesis Photosynthesis Tricarboxylic acid cycle Secondary metabolism Transcriptome analysis |
| title | Comparative transcriptome analysis of differentially expressed genes related to the physiological changes of yellow-green leaf mutant of maize |
| title_full | Comparative transcriptome analysis of differentially expressed genes related to the physiological changes of yellow-green leaf mutant of maize |
| title_fullStr | Comparative transcriptome analysis of differentially expressed genes related to the physiological changes of yellow-green leaf mutant of maize |
| title_full_unstemmed | Comparative transcriptome analysis of differentially expressed genes related to the physiological changes of yellow-green leaf mutant of maize |
| title_short | Comparative transcriptome analysis of differentially expressed genes related to the physiological changes of yellow-green leaf mutant of maize |
| title_sort | comparative transcriptome analysis of differentially expressed genes related to the physiological changes of yellow green leaf mutant of maize |
| topic | Yellow-green leaf Chlorophyll biosynthesis Photosynthesis Tricarboxylic acid cycle Secondary metabolism Transcriptome analysis |
| url | https://peerj.com/articles/10567.pdf |
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