Effect of Physiological Concentrations of Vitamin C on the Inhibitation of Hydroxyl Radical Induced Light Emission from Fe<sup>2+</sup>-EGTA-H<sub>2</sub>O<sub>2</sub> and Fe<sup>3+</sup>-EGTA-H<sub>2</sub>O<sub>2</sub> Systems In Vitro
Ascorbic acid (AA) has antioxidant properties. However, in the presence of Fe<sup>2+</sup>/Fe<sup>3+</sup> ions and H<sub>2</sub>O<sub>2</sub>, it may behave as a pro-oxidant by accelerating and enhancing the formation of hydroxyl radicals (<sup>...
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| author | Michal Nowak Wieslaw Tryniszewski Agata Sarniak Anna Wlodarczyk Piotr J. Nowak Dariusz Nowak |
| author_facet | Michal Nowak Wieslaw Tryniszewski Agata Sarniak Anna Wlodarczyk Piotr J. Nowak Dariusz Nowak |
| author_sort | Michal Nowak |
| collection | DOAJ |
| description | Ascorbic acid (AA) has antioxidant properties. However, in the presence of Fe<sup>2+</sup>/Fe<sup>3+</sup> ions and H<sub>2</sub>O<sub>2</sub>, it may behave as a pro-oxidant by accelerating and enhancing the formation of hydroxyl radicals (<sup>•</sup>OH). Therefore, in this study we evaluated the effect of AA at concentrations of 1 to 200 µmol/L on <sup>•</sup>OH-induced light emission (at a pH of 7.4 and temperature of 37 °C) from 92.6 µmol/L Fe<sup>2+</sup>—185.2 µmol/L EGTA (ethylene glycol-bis (β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid)—2.6 mmol/L H<sub>2</sub>O<sub>2</sub>, and 92.6 µmol/L Fe<sup>3+</sup>—185.2 µmol/L EGTA—2.6 mmol/L H<sub>2</sub>O<sub>2</sub> systems. Dehydroascorbic acid (DHAA) at the same range of concentrations served as the reference compound. Light emission was measured with multitube luminometer (AutoLumat Plus LB 953) for 120 s after automatic injection of H<sub>2</sub>O<sub>2</sub>. AA at concentrations of 1 to 50 µmol/L and of 1 to 75 µmol/L completely inhibited light emission from Fe<sup>2+</sup>-EGTA-H<sub>2</sub>O<sub>2</sub> and Fe<sup>3+</sup>-EGTA-H<sub>2</sub>O<sub>2</sub>, respectively. Concentrations of 100 and 200 µmol/L did not affect chemiluminescence of Fe<sup>3+</sup>-EGTA-H<sub>2</sub>O<sub>2</sub> but tended to increase light emission from Fe<sup>2+</sup>-EGTA-H<sub>2</sub>O<sub>2</sub>. DHAA at concentrations of 1 to 100 µmol/L had no effect on chemiluminescence of both systems. These results indicate that AA at physiological concentrations exhibits strong antioxidant activity in the presence of chelated iron and H<sub>2</sub>O<sub>2</sub>. |
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| spelling | doaj.art-2730e29c12674529b51f564d98e01dfc2023-11-21T13:45:33ZengMDPI AGMolecules1420-30492021-04-01267199310.3390/molecules26071993Effect of Physiological Concentrations of Vitamin C on the Inhibitation of Hydroxyl Radical Induced Light Emission from Fe<sup>2+</sup>-EGTA-H<sub>2</sub>O<sub>2</sub> and Fe<sup>3+</sup>-EGTA-H<sub>2</sub>O<sub>2</sub> Systems In VitroMichal Nowak0Wieslaw Tryniszewski1Agata Sarniak2Anna Wlodarczyk3Piotr J. Nowak4Dariusz Nowak5Radiation Protection, University Hospital No. 2, Medical University of Lodz, Zeromskiego 113, 90-549 Lodz, PolandDepartment of Radiological and Isotopic Diagnostics and Therapy, Medical University of Lodz, Zeromskiego 113, 90-549 Lodz, PolandDepartment of General Physiology, Medical University of Lodz, Mazowiecka 6/8, 92-215 Lodz, PolandDepartment of Sleep Medicine and Metabolic Disorders, Medical University of Lodz, Mazowiecka 6/8, 92-215 Lodz, PolandDepartment of Nephrology, Hypertension, and Kidney Transplantation, Medical University of Lodz, Pomorska 251, 92-213 Lodz, PolandDepartment of Clinical Physiology, Medical University of Lodz, Mazowiecka 6/8, 92-215 Lodz, PolandAscorbic acid (AA) has antioxidant properties. However, in the presence of Fe<sup>2+</sup>/Fe<sup>3+</sup> ions and H<sub>2</sub>O<sub>2</sub>, it may behave as a pro-oxidant by accelerating and enhancing the formation of hydroxyl radicals (<sup>•</sup>OH). Therefore, in this study we evaluated the effect of AA at concentrations of 1 to 200 µmol/L on <sup>•</sup>OH-induced light emission (at a pH of 7.4 and temperature of 37 °C) from 92.6 µmol/L Fe<sup>2+</sup>—185.2 µmol/L EGTA (ethylene glycol-bis (β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid)—2.6 mmol/L H<sub>2</sub>O<sub>2</sub>, and 92.6 µmol/L Fe<sup>3+</sup>—185.2 µmol/L EGTA—2.6 mmol/L H<sub>2</sub>O<sub>2</sub> systems. Dehydroascorbic acid (DHAA) at the same range of concentrations served as the reference compound. Light emission was measured with multitube luminometer (AutoLumat Plus LB 953) for 120 s after automatic injection of H<sub>2</sub>O<sub>2</sub>. AA at concentrations of 1 to 50 µmol/L and of 1 to 75 µmol/L completely inhibited light emission from Fe<sup>2+</sup>-EGTA-H<sub>2</sub>O<sub>2</sub> and Fe<sup>3+</sup>-EGTA-H<sub>2</sub>O<sub>2</sub>, respectively. Concentrations of 100 and 200 µmol/L did not affect chemiluminescence of Fe<sup>3+</sup>-EGTA-H<sub>2</sub>O<sub>2</sub> but tended to increase light emission from Fe<sup>2+</sup>-EGTA-H<sub>2</sub>O<sub>2</sub>. DHAA at concentrations of 1 to 100 µmol/L had no effect on chemiluminescence of both systems. These results indicate that AA at physiological concentrations exhibits strong antioxidant activity in the presence of chelated iron and H<sub>2</sub>O<sub>2</sub>.https://www.mdpi.com/1420-3049/26/7/1993ascorbic aciddehydroascorbic acidchemiluminescenceFenton systemantioxidant activitypro-oxidant activity |
| spellingShingle | Michal Nowak Wieslaw Tryniszewski Agata Sarniak Anna Wlodarczyk Piotr J. Nowak Dariusz Nowak Effect of Physiological Concentrations of Vitamin C on the Inhibitation of Hydroxyl Radical Induced Light Emission from Fe<sup>2+</sup>-EGTA-H<sub>2</sub>O<sub>2</sub> and Fe<sup>3+</sup>-EGTA-H<sub>2</sub>O<sub>2</sub> Systems In Vitro Molecules ascorbic acid dehydroascorbic acid chemiluminescence Fenton system antioxidant activity pro-oxidant activity |
| title | Effect of Physiological Concentrations of Vitamin C on the Inhibitation of Hydroxyl Radical Induced Light Emission from Fe<sup>2+</sup>-EGTA-H<sub>2</sub>O<sub>2</sub> and Fe<sup>3+</sup>-EGTA-H<sub>2</sub>O<sub>2</sub> Systems In Vitro |
| title_full | Effect of Physiological Concentrations of Vitamin C on the Inhibitation of Hydroxyl Radical Induced Light Emission from Fe<sup>2+</sup>-EGTA-H<sub>2</sub>O<sub>2</sub> and Fe<sup>3+</sup>-EGTA-H<sub>2</sub>O<sub>2</sub> Systems In Vitro |
| title_fullStr | Effect of Physiological Concentrations of Vitamin C on the Inhibitation of Hydroxyl Radical Induced Light Emission from Fe<sup>2+</sup>-EGTA-H<sub>2</sub>O<sub>2</sub> and Fe<sup>3+</sup>-EGTA-H<sub>2</sub>O<sub>2</sub> Systems In Vitro |
| title_full_unstemmed | Effect of Physiological Concentrations of Vitamin C on the Inhibitation of Hydroxyl Radical Induced Light Emission from Fe<sup>2+</sup>-EGTA-H<sub>2</sub>O<sub>2</sub> and Fe<sup>3+</sup>-EGTA-H<sub>2</sub>O<sub>2</sub> Systems In Vitro |
| title_short | Effect of Physiological Concentrations of Vitamin C on the Inhibitation of Hydroxyl Radical Induced Light Emission from Fe<sup>2+</sup>-EGTA-H<sub>2</sub>O<sub>2</sub> and Fe<sup>3+</sup>-EGTA-H<sub>2</sub>O<sub>2</sub> Systems In Vitro |
| title_sort | effect of physiological concentrations of vitamin c on the inhibitation of hydroxyl radical induced light emission from fe sup 2 sup egta h sub 2 sub o sub 2 sub and fe sup 3 sup egta h sub 2 sub o sub 2 sub systems in vitro |
| topic | ascorbic acid dehydroascorbic acid chemiluminescence Fenton system antioxidant activity pro-oxidant activity |
| url | https://www.mdpi.com/1420-3049/26/7/1993 |
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