Connectivity from OR37 expressing olfactory sensory neurons to distinct cell types in the hypothalamus
Olfactory sensory neurons which express a member from the OR37 subfamily of odorant receptor genes are wired to the main olfactory bulb in a unique monoglomerular fashion; from these glomeruli an untypical connectivity into higher brain centers exists. In the present study we have investigated by Di...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2012-11-01
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| Series: | Frontiers in Neural Circuits |
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| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fncir.2012.00084/full |
| _version_ | 1818136669596942336 |
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| author | Andrea eBader Bettina eKlein Heinz eBreer Joerg eStrotmann |
| author_facet | Andrea eBader Bettina eKlein Heinz eBreer Joerg eStrotmann |
| author_sort | Andrea eBader |
| collection | DOAJ |
| description | Olfactory sensory neurons which express a member from the OR37 subfamily of odorant receptor genes are wired to the main olfactory bulb in a unique monoglomerular fashion; from these glomeruli an untypical connectivity into higher brain centers exists. In the present study we have investigated by DiI and transsynaptic tracing approaches how the connection pattern from these glomeruli into distinct hypothalamic nuclei is organized. The application of DiI onto the ventral domain of the bulb which harbors the OR37 glomeruli resulted in the labeling of fibers within the paraventricular and supraoptic nucleus of the hypothalamus; some of these fibers were covered with varicose-like structures. No DiI-labeled cell somata were detectable in these nuclei. The data indicate that projection neurons which originate in the OR37 region of the main olfactory bulb form direct connections into these nuclei. The cells that were labeled by the transsynaptic tracer WGA in these nuclei were further characterized. Their distribution pattern in the paraventricular nucleus was reminiscent of cells which produce distinct neuropeptides. Double labeling experiments confirmed that they contained vasopressin, but not the related neuropeptide oxytocin. Morphological analysis revealed that they comprise of magno- and parvocellular cells. A comparative investigation of the WGA-positive cells in the supraoptic nucleus demonstrated that these were vasopressin-positive, as well, whereas oxytocin-producing cells of this nucleus also contained no transsynaptic tracer. Together, the data demonstrate a connectivity from OR37 expressing sensory neurons to distinct hypothalamic neurons with the same neuropeptide content. |
| first_indexed | 2024-12-11T09:44:05Z |
| format | Article |
| id | doaj.art-27336cb6ec0747b18827b481da9e11d0 |
| institution | Directory Open Access Journal |
| issn | 1662-5110 |
| language | English |
| last_indexed | 2024-12-11T09:44:05Z |
| publishDate | 2012-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Neural Circuits |
| spelling | doaj.art-27336cb6ec0747b18827b481da9e11d02022-12-22T01:12:35ZengFrontiers Media S.A.Frontiers in Neural Circuits1662-51102012-11-01610.3389/fncir.2012.0008436315Connectivity from OR37 expressing olfactory sensory neurons to distinct cell types in the hypothalamusAndrea eBader0Bettina eKlein1Heinz eBreer2Joerg eStrotmann3University of HohenheimUniversity of HohenheimUniversity of HohenheimUniversity of HohenheimOlfactory sensory neurons which express a member from the OR37 subfamily of odorant receptor genes are wired to the main olfactory bulb in a unique monoglomerular fashion; from these glomeruli an untypical connectivity into higher brain centers exists. In the present study we have investigated by DiI and transsynaptic tracing approaches how the connection pattern from these glomeruli into distinct hypothalamic nuclei is organized. The application of DiI onto the ventral domain of the bulb which harbors the OR37 glomeruli resulted in the labeling of fibers within the paraventricular and supraoptic nucleus of the hypothalamus; some of these fibers were covered with varicose-like structures. No DiI-labeled cell somata were detectable in these nuclei. The data indicate that projection neurons which originate in the OR37 region of the main olfactory bulb form direct connections into these nuclei. The cells that were labeled by the transsynaptic tracer WGA in these nuclei were further characterized. Their distribution pattern in the paraventricular nucleus was reminiscent of cells which produce distinct neuropeptides. Double labeling experiments confirmed that they contained vasopressin, but not the related neuropeptide oxytocin. Morphological analysis revealed that they comprise of magno- and parvocellular cells. A comparative investigation of the WGA-positive cells in the supraoptic nucleus demonstrated that these were vasopressin-positive, as well, whereas oxytocin-producing cells of this nucleus also contained no transsynaptic tracer. Together, the data demonstrate a connectivity from OR37 expressing sensory neurons to distinct hypothalamic neurons with the same neuropeptide content.http://journal.frontiersin.org/Journal/10.3389/fncir.2012.00084/fullSupraoptic Nucleusvasopressinparaventricular nucleusOlfactionwiringOR37 |
| spellingShingle | Andrea eBader Bettina eKlein Heinz eBreer Joerg eStrotmann Connectivity from OR37 expressing olfactory sensory neurons to distinct cell types in the hypothalamus Frontiers in Neural Circuits Supraoptic Nucleus vasopressin paraventricular nucleus Olfaction wiring OR37 |
| title | Connectivity from OR37 expressing olfactory sensory neurons to distinct cell types in the hypothalamus |
| title_full | Connectivity from OR37 expressing olfactory sensory neurons to distinct cell types in the hypothalamus |
| title_fullStr | Connectivity from OR37 expressing olfactory sensory neurons to distinct cell types in the hypothalamus |
| title_full_unstemmed | Connectivity from OR37 expressing olfactory sensory neurons to distinct cell types in the hypothalamus |
| title_short | Connectivity from OR37 expressing olfactory sensory neurons to distinct cell types in the hypothalamus |
| title_sort | connectivity from or37 expressing olfactory sensory neurons to distinct cell types in the hypothalamus |
| topic | Supraoptic Nucleus vasopressin paraventricular nucleus Olfaction wiring OR37 |
| url | http://journal.frontiersin.org/Journal/10.3389/fncir.2012.00084/full |
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