Demonstration of a Label-Free and Low-Cost Optical Cavity-Based Biosensor Using Streptavidin and C-Reactive Protein
An optical cavity-based biosensor (OCB) has been developed for point-of-care (POC) applications. This label-free biosensor employs low-cost components and simple fabrication processes to lower the overall cost while achieving high sensitivity using a differential detection method. To experimentally...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-12-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/11/1/4 |
| _version_ | 1797543683953786880 |
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| author | Donggee Rho Seunghyun Kim |
| author_facet | Donggee Rho Seunghyun Kim |
| author_sort | Donggee Rho |
| collection | DOAJ |
| description | An optical cavity-based biosensor (OCB) has been developed for point-of-care (POC) applications. This label-free biosensor employs low-cost components and simple fabrication processes to lower the overall cost while achieving high sensitivity using a differential detection method. To experimentally demonstrate its limit of detection (LOD), we conducted biosensing experiments with streptavidin and C-reactive protein (CRP). The optical cavity structure was optimized further for better sensitivity and easier fluid control. We utilized the polymer swelling property to fine-tune the optical cavity width, which significantly improved the success rate to produce measurable samples. Four different concentrations of streptavidin were tested in triplicate, and the LOD of the OCB was determined to be 1.35 nM. The OCB also successfully detected three different concentrations of human CRP using biotinylated CRP antibody. The LOD for CRP detection was 377 pM. All measurements were done using a small sample volume of 15 µL within 30 min. By reducing the sensing area, improving the functionalization and passivation processes, and increasing the sample volume, the LOD of the OCB are estimated to be reduced further to the femto-molar range. Overall, the demonstrated capability of the OCB in the present work shows great potential to be used as a promising POC biosensor. |
| first_indexed | 2024-03-10T13:49:09Z |
| format | Article |
| id | doaj.art-2734dba83fb14c2bb8c56d4606636251 |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-10T13:49:09Z |
| publishDate | 2020-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-2734dba83fb14c2bb8c56d46066362512023-11-21T02:20:48ZengMDPI AGBiosensors2079-63742020-12-01111410.3390/bios11010004Demonstration of a Label-Free and Low-Cost Optical Cavity-Based Biosensor Using Streptavidin and C-Reactive ProteinDonggee Rho0Seunghyun Kim1Electrical and Computer Engineering Department, Baylor University, One Bear Place #97356, Waco, TX 76798, USAElectrical and Computer Engineering Department, Baylor University, One Bear Place #97356, Waco, TX 76798, USAAn optical cavity-based biosensor (OCB) has been developed for point-of-care (POC) applications. This label-free biosensor employs low-cost components and simple fabrication processes to lower the overall cost while achieving high sensitivity using a differential detection method. To experimentally demonstrate its limit of detection (LOD), we conducted biosensing experiments with streptavidin and C-reactive protein (CRP). The optical cavity structure was optimized further for better sensitivity and easier fluid control. We utilized the polymer swelling property to fine-tune the optical cavity width, which significantly improved the success rate to produce measurable samples. Four different concentrations of streptavidin were tested in triplicate, and the LOD of the OCB was determined to be 1.35 nM. The OCB also successfully detected three different concentrations of human CRP using biotinylated CRP antibody. The LOD for CRP detection was 377 pM. All measurements were done using a small sample volume of 15 µL within 30 min. By reducing the sensing area, improving the functionalization and passivation processes, and increasing the sample volume, the LOD of the OCB are estimated to be reduced further to the femto-molar range. Overall, the demonstrated capability of the OCB in the present work shows great potential to be used as a promising POC biosensor.https://www.mdpi.com/2079-6374/11/1/4biosensorsoptical cavity-based biosensorbiomarker detection |
| spellingShingle | Donggee Rho Seunghyun Kim Demonstration of a Label-Free and Low-Cost Optical Cavity-Based Biosensor Using Streptavidin and C-Reactive Protein Biosensors biosensors optical cavity-based biosensor biomarker detection |
| title | Demonstration of a Label-Free and Low-Cost Optical Cavity-Based Biosensor Using Streptavidin and C-Reactive Protein |
| title_full | Demonstration of a Label-Free and Low-Cost Optical Cavity-Based Biosensor Using Streptavidin and C-Reactive Protein |
| title_fullStr | Demonstration of a Label-Free and Low-Cost Optical Cavity-Based Biosensor Using Streptavidin and C-Reactive Protein |
| title_full_unstemmed | Demonstration of a Label-Free and Low-Cost Optical Cavity-Based Biosensor Using Streptavidin and C-Reactive Protein |
| title_short | Demonstration of a Label-Free and Low-Cost Optical Cavity-Based Biosensor Using Streptavidin and C-Reactive Protein |
| title_sort | demonstration of a label free and low cost optical cavity based biosensor using streptavidin and c reactive protein |
| topic | biosensors optical cavity-based biosensor biomarker detection |
| url | https://www.mdpi.com/2079-6374/11/1/4 |
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