The Use of RAPD Markers in the Study of Polymorphism of Mountain Populations of Dandelion Officinalis
Molecular markers are used in plant science in a variety of ways. The method of molecular genome marking based on RAPD-PCR makes it possible to determine the genetic status of populations and establish intrapopulation relationships. In this work, we studied the genetic polymorphism of the dandelion...
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| Format: | Article |
| Language: | English |
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EDP Sciences
2023-01-01
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| Series: | BIO Web of Conferences |
| Online Access: | https://www.bio-conferences.org/articles/bioconf/pdf/2023/08/bioconf_ase2023_07003.pdf |
| _version_ | 1797756280629100544 |
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| author | Bisultanova Z. I. Dzhambetova P. M. Dzhambetova L. M. |
| author_facet | Bisultanova Z. I. Dzhambetova P. M. Dzhambetova L. M. |
| author_sort | Bisultanova Z. I. |
| collection | DOAJ |
| description | Molecular markers are used in plant science in a variety of ways. The method of molecular genome marking based on RAPD-PCR makes it possible to determine the genetic status of populations and establish intrapopulation relationships. In this work, we studied the genetic polymorphism of the dandelion Taraxacum officionale using RAPD markers. Five markers of random amplified polymorphic DNA (RAPD) were used to determine the genetic diversity and genetic relationship between two populations of T. officionale growing on the slopes of the Andean Range. A total of 26 fragments were amplified, and 25 of them were polymorphic, with an average of 6 amplified bands per primer. The percentage of total polymorphism was 96.1%. The size of the amplified fragments varied from 150 to 1200 bp. It has been shown that molecular genetic analysis makes it possible to identify RAPD markers of the dandelion genome, which can be used for population identification of genotypes. |
| first_indexed | 2024-03-12T17:59:12Z |
| format | Article |
| id | doaj.art-2736b3672e2549cd8de47122e146a00c |
| institution | Directory Open Access Journal |
| issn | 2117-4458 |
| language | English |
| last_indexed | 2024-03-12T17:59:12Z |
| publishDate | 2023-01-01 |
| publisher | EDP Sciences |
| record_format | Article |
| series | BIO Web of Conferences |
| spelling | doaj.art-2736b3672e2549cd8de47122e146a00c2023-08-02T13:13:41ZengEDP SciencesBIO Web of Conferences2117-44582023-01-01630700310.1051/bioconf/20236307003bioconf_ase2023_07003The Use of RAPD Markers in the Study of Polymorphism of Mountain Populations of Dandelion OfficinalisBisultanova Z. I.0Dzhambetova P. M.1Dzhambetova L. M.2Kadyrov Chechen State UniversityKadyrov Chechen State UniversityKadyrov Chechen State UniversityMolecular markers are used in plant science in a variety of ways. The method of molecular genome marking based on RAPD-PCR makes it possible to determine the genetic status of populations and establish intrapopulation relationships. In this work, we studied the genetic polymorphism of the dandelion Taraxacum officionale using RAPD markers. Five markers of random amplified polymorphic DNA (RAPD) were used to determine the genetic diversity and genetic relationship between two populations of T. officionale growing on the slopes of the Andean Range. A total of 26 fragments were amplified, and 25 of them were polymorphic, with an average of 6 amplified bands per primer. The percentage of total polymorphism was 96.1%. The size of the amplified fragments varied from 150 to 1200 bp. It has been shown that molecular genetic analysis makes it possible to identify RAPD markers of the dandelion genome, which can be used for population identification of genotypes.https://www.bio-conferences.org/articles/bioconf/pdf/2023/08/bioconf_ase2023_07003.pdf |
| spellingShingle | Bisultanova Z. I. Dzhambetova P. M. Dzhambetova L. M. The Use of RAPD Markers in the Study of Polymorphism of Mountain Populations of Dandelion Officinalis BIO Web of Conferences |
| title | The Use of RAPD Markers in the Study of Polymorphism of Mountain Populations of Dandelion Officinalis |
| title_full | The Use of RAPD Markers in the Study of Polymorphism of Mountain Populations of Dandelion Officinalis |
| title_fullStr | The Use of RAPD Markers in the Study of Polymorphism of Mountain Populations of Dandelion Officinalis |
| title_full_unstemmed | The Use of RAPD Markers in the Study of Polymorphism of Mountain Populations of Dandelion Officinalis |
| title_short | The Use of RAPD Markers in the Study of Polymorphism of Mountain Populations of Dandelion Officinalis |
| title_sort | use of rapd markers in the study of polymorphism of mountain populations of dandelion officinalis |
| url | https://www.bio-conferences.org/articles/bioconf/pdf/2023/08/bioconf_ase2023_07003.pdf |
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