Quantitative characterization of the B cell receptor repertoires of human immunized with commercial rabies virus vaccine
Humoral immunity is crucial for an efficient host immune response against rabies virus (RABV) infection. But the B cell receptor (BCR) repertoire in human after RABV vaccine immunization remained unclear. To study the BCR repertoires in peripheral blood mononuclear cells (PBMCs) of human immunized w...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2021-08-01
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| Series: | Human Vaccines & Immunotherapeutics |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/21645515.2021.1893576 |
| _version_ | 1797674465141719040 |
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| author | Pingsen Zhao Sharula Guo Zhixiong Zhong Songtao Yang Xianzhu Xia |
| author_facet | Pingsen Zhao Sharula Guo Zhixiong Zhong Songtao Yang Xianzhu Xia |
| author_sort | Pingsen Zhao |
| collection | DOAJ |
| description | Humoral immunity is crucial for an efficient host immune response against rabies virus (RABV) infection. But the B cell receptor (BCR) repertoire in human after RABV vaccine immunization remained unclear. To study the BCR repertoires in peripheral blood mononuclear cells (PBMCs) of human immunized with rabies virus vaccine. In this study, we conducted BCR complementarity determining region 3 (CDR3) repertoires in 4 healthy volunteers before and after immunization with RABV vaccine by high-throughput sequencing. The bioinformatics analysis process was performed. The results showed that RABV vaccination changed the BCR diversity and the usage of V/J gene segments, as well as V-J pairing. B cell clone expansion was induced by the vaccination and sequences of high expand CDR3 aa clones were identified. To the best of our knowledge, we firstly quantitative characterized B cell receptor repertoire of human immunized with c rabies virus vaccine. It might provide us with new insights into B cell receptor condition after RABV vaccination. |
| first_indexed | 2024-03-11T22:00:25Z |
| format | Article |
| id | doaj.art-27435786f635455e9c16d2878484db07 |
| institution | Directory Open Access Journal |
| issn | 2164-5515 2164-554X |
| language | English |
| last_indexed | 2024-03-11T22:00:25Z |
| publishDate | 2021-08-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Human Vaccines & Immunotherapeutics |
| spelling | doaj.art-27435786f635455e9c16d2878484db072023-09-25T11:17:05ZengTaylor & Francis GroupHuman Vaccines & Immunotherapeutics2164-55152164-554X2021-08-011782538254610.1080/21645515.2021.18935761893576Quantitative characterization of the B cell receptor repertoires of human immunized with commercial rabies virus vaccinePingsen Zhao0Sharula Guo1Zhixiong Zhong2Songtao Yang3Xianzhu Xia4Yuebei People’s Hospital, Shantou University Medical CollegeShantou University Medical CollegeMeizhou Hospital Affiliated to Sun Yat-sen UniversityAcademy of Military Medical SciencesShantou University Medical CollegeHumoral immunity is crucial for an efficient host immune response against rabies virus (RABV) infection. But the B cell receptor (BCR) repertoire in human after RABV vaccine immunization remained unclear. To study the BCR repertoires in peripheral blood mononuclear cells (PBMCs) of human immunized with rabies virus vaccine. In this study, we conducted BCR complementarity determining region 3 (CDR3) repertoires in 4 healthy volunteers before and after immunization with RABV vaccine by high-throughput sequencing. The bioinformatics analysis process was performed. The results showed that RABV vaccination changed the BCR diversity and the usage of V/J gene segments, as well as V-J pairing. B cell clone expansion was induced by the vaccination and sequences of high expand CDR3 aa clones were identified. To the best of our knowledge, we firstly quantitative characterized B cell receptor repertoire of human immunized with c rabies virus vaccine. It might provide us with new insights into B cell receptor condition after RABV vaccination.http://dx.doi.org/10.1080/21645515.2021.1893576rabies virusvaccineb cell receptor repertoireimmune repertoire sequencingcomplementarity-determining region |
| spellingShingle | Pingsen Zhao Sharula Guo Zhixiong Zhong Songtao Yang Xianzhu Xia Quantitative characterization of the B cell receptor repertoires of human immunized with commercial rabies virus vaccine Human Vaccines & Immunotherapeutics rabies virus vaccine b cell receptor repertoire immune repertoire sequencing complementarity-determining region |
| title | Quantitative characterization of the B cell receptor repertoires of human immunized with commercial rabies virus vaccine |
| title_full | Quantitative characterization of the B cell receptor repertoires of human immunized with commercial rabies virus vaccine |
| title_fullStr | Quantitative characterization of the B cell receptor repertoires of human immunized with commercial rabies virus vaccine |
| title_full_unstemmed | Quantitative characterization of the B cell receptor repertoires of human immunized with commercial rabies virus vaccine |
| title_short | Quantitative characterization of the B cell receptor repertoires of human immunized with commercial rabies virus vaccine |
| title_sort | quantitative characterization of the b cell receptor repertoires of human immunized with commercial rabies virus vaccine |
| topic | rabies virus vaccine b cell receptor repertoire immune repertoire sequencing complementarity-determining region |
| url | http://dx.doi.org/10.1080/21645515.2021.1893576 |
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