Harnessing multiplex PCR assay targeting specific mitochondrial DNA elements for simultaneous identification of antelope species in Cornu Saigae Tataricae
A multiplex PCR assay was developed to simultaneously differentiate four antelope species and identify adulteration in Cornu Saigae Tataricae. Four novel primer sets were designed with high inter-species specificity and intra-species stability. Limit of detection was estimated to be 10 ng of genomes...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2019-07-01
|
| Series: | Mitochondrial DNA. Part B. Resources |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/23802359.2019.1666667 |
| _version_ | 1827773314261581824 |
|---|---|
| author | Yufei Chen Yaya Yang Yinhuan Qian Roselyn Tehzee Gblinwon Zhaoqun Jiao Liqun Chen Ling Lin Yang Zheng Huan Yang Yuping Shen |
| author_facet | Yufei Chen Yaya Yang Yinhuan Qian Roselyn Tehzee Gblinwon Zhaoqun Jiao Liqun Chen Ling Lin Yang Zheng Huan Yang Yuping Shen |
| author_sort | Yufei Chen |
| collection | DOAJ |
| description | A multiplex PCR assay was developed to simultaneously differentiate four antelope species and identify adulteration in Cornu Saigae Tataricae. Four novel primer sets were designed with high inter-species specificity and intra-species stability. Limit of detection was estimated to be 10 ng of genomes. When a mixture of antelope hornand fake species was assayed, it exhibited powerful differentiation capability. 5 out of 12 batches of commercialproducts were identified to be counterfeited or adulterated with Ovis aries Linnaeus and/or Capra hircus Linnaeus. It is readily applicable in routine analysis for identification of sham or adulterants of Cornu Saigae Tataricae. |
| first_indexed | 2024-03-11T13:20:17Z |
| format | Article |
| id | doaj.art-2743d91bbac448e784e0e8df261c2103 |
| institution | Directory Open Access Journal |
| issn | 2380-2359 |
| language | English |
| last_indexed | 2024-03-11T13:20:17Z |
| publishDate | 2019-07-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Mitochondrial DNA. Part B. Resources |
| spelling | doaj.art-2743d91bbac448e784e0e8df261c21032023-11-03T10:28:38ZengTaylor & Francis GroupMitochondrial DNA. Part B. Resources2380-23592019-07-01423222322610.1080/23802359.2019.16666671666667Harnessing multiplex PCR assay targeting specific mitochondrial DNA elements for simultaneous identification of antelope species in Cornu Saigae TataricaeYufei Chen0Yaya Yang1Yinhuan Qian2Roselyn Tehzee Gblinwon3Zhaoqun Jiao4Liqun Chen5Ling Lin6Yang Zheng7Huan Yang8Yuping Shen9Jiangsu UniversityJiangsu UniversityZhenjiang Hospital of Traditional Chinese MedicineJiangsu UniversityJiangsu UniversityJiangsu UniversityJiangsu UniversityJiangsu UniversityJiangsu UniversityJiangsu UniversityA multiplex PCR assay was developed to simultaneously differentiate four antelope species and identify adulteration in Cornu Saigae Tataricae. Four novel primer sets were designed with high inter-species specificity and intra-species stability. Limit of detection was estimated to be 10 ng of genomes. When a mixture of antelope hornand fake species was assayed, it exhibited powerful differentiation capability. 5 out of 12 batches of commercialproducts were identified to be counterfeited or adulterated with Ovis aries Linnaeus and/or Capra hircus Linnaeus. It is readily applicable in routine analysis for identification of sham or adulterants of Cornu Saigae Tataricae.http://dx.doi.org/10.1080/23802359.2019.1666667mitochondrial dnaspecies-specific primermultiplex pcr assayantelope hornanimal-derived chinese medicinespecies identification |
| spellingShingle | Yufei Chen Yaya Yang Yinhuan Qian Roselyn Tehzee Gblinwon Zhaoqun Jiao Liqun Chen Ling Lin Yang Zheng Huan Yang Yuping Shen Harnessing multiplex PCR assay targeting specific mitochondrial DNA elements for simultaneous identification of antelope species in Cornu Saigae Tataricae Mitochondrial DNA. Part B. Resources mitochondrial dna species-specific primer multiplex pcr assay antelope horn animal-derived chinese medicine species identification |
| title | Harnessing multiplex PCR assay targeting specific mitochondrial DNA elements for simultaneous identification of antelope species in Cornu Saigae Tataricae |
| title_full | Harnessing multiplex PCR assay targeting specific mitochondrial DNA elements for simultaneous identification of antelope species in Cornu Saigae Tataricae |
| title_fullStr | Harnessing multiplex PCR assay targeting specific mitochondrial DNA elements for simultaneous identification of antelope species in Cornu Saigae Tataricae |
| title_full_unstemmed | Harnessing multiplex PCR assay targeting specific mitochondrial DNA elements for simultaneous identification of antelope species in Cornu Saigae Tataricae |
| title_short | Harnessing multiplex PCR assay targeting specific mitochondrial DNA elements for simultaneous identification of antelope species in Cornu Saigae Tataricae |
| title_sort | harnessing multiplex pcr assay targeting specific mitochondrial dna elements for simultaneous identification of antelope species in cornu saigae tataricae |
| topic | mitochondrial dna species-specific primer multiplex pcr assay antelope horn animal-derived chinese medicine species identification |
| url | http://dx.doi.org/10.1080/23802359.2019.1666667 |
| work_keys_str_mv | AT yufeichen harnessingmultiplexpcrassaytargetingspecificmitochondrialdnaelementsforsimultaneousidentificationofantelopespeciesincornusaigaetataricae AT yayayang harnessingmultiplexpcrassaytargetingspecificmitochondrialdnaelementsforsimultaneousidentificationofantelopespeciesincornusaigaetataricae AT yinhuanqian harnessingmultiplexpcrassaytargetingspecificmitochondrialdnaelementsforsimultaneousidentificationofantelopespeciesincornusaigaetataricae AT roselyntehzeegblinwon harnessingmultiplexpcrassaytargetingspecificmitochondrialdnaelementsforsimultaneousidentificationofantelopespeciesincornusaigaetataricae AT zhaoqunjiao harnessingmultiplexpcrassaytargetingspecificmitochondrialdnaelementsforsimultaneousidentificationofantelopespeciesincornusaigaetataricae AT liqunchen harnessingmultiplexpcrassaytargetingspecificmitochondrialdnaelementsforsimultaneousidentificationofantelopespeciesincornusaigaetataricae AT linglin harnessingmultiplexpcrassaytargetingspecificmitochondrialdnaelementsforsimultaneousidentificationofantelopespeciesincornusaigaetataricae AT yangzheng harnessingmultiplexpcrassaytargetingspecificmitochondrialdnaelementsforsimultaneousidentificationofantelopespeciesincornusaigaetataricae AT huanyang harnessingmultiplexpcrassaytargetingspecificmitochondrialdnaelementsforsimultaneousidentificationofantelopespeciesincornusaigaetataricae AT yupingshen harnessingmultiplexpcrassaytargetingspecificmitochondrialdnaelementsforsimultaneousidentificationofantelopespeciesincornusaigaetataricae |