L-lactic acid production from Lactobacillus casei by solid state fermentation using rice straw

In order to make full use of rice straw (RS) produced at large quantity in China and to reduce the production cost of L-lactic acid, attempts were made to utilize the hydrolysate of RS as sole carbon source and the lignocellulose as inert support for producing L-lactic acid using solid state ferment...

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Main Authors: Risheng, Yao, Benkun Qi
Format: Article
Language:English
Published: North Carolina State University 2007-01-01
Series:BioResources
Subjects:
Online Access:http://ojs.cnr.ncsu.edu/index.php/BioRes/article/viewFile/BioRes_2_3_419_429_Qi_Yao_LacticAcid_RiceStraw/58
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author Risheng, Yao
Benkun Qi
author_facet Risheng, Yao
Benkun Qi
author_sort Risheng, Yao
collection DOAJ
description In order to make full use of rice straw (RS) produced at large quantity in China and to reduce the production cost of L-lactic acid, attempts were made to utilize the hydrolysate of RS as sole carbon source and the lignocellulose as inert support for producing L-lactic acid using solid state fermentation (SSF). The pretreated rice straw was enzymatically hydro- lyzed by cellulase, and the hydrolyzate, containing reducing sugars supplemented with a minimum of (NH4)2SO4, MnSO4, and yeast extract, was used as moistening agent to impregnate 5g of RS, which was used as the inert support for SSF. Maximum L-lactic acid production of 3.467g per 5g of support was obtained at 37 oC, using Lactobacillus casei as inoculum, after 5 days of fermentation with optimized process parameters such as 72% moisture content, 4g per 5g support of reducing sugars, 2.5ml per 5g support of inoculum size, 3g per 5g support of CaCO3, and pH 6.5.
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spelling doaj.art-27453563de8f41dca4dfc531c6cc0ff82022-12-21T18:11:14ZengNorth Carolina State UniversityBioResources1930-21262007-01-0123419429L-lactic acid production from Lactobacillus casei by solid state fermentation using rice strawRisheng, YaoBenkun QiIn order to make full use of rice straw (RS) produced at large quantity in China and to reduce the production cost of L-lactic acid, attempts were made to utilize the hydrolysate of RS as sole carbon source and the lignocellulose as inert support for producing L-lactic acid using solid state fermentation (SSF). The pretreated rice straw was enzymatically hydro- lyzed by cellulase, and the hydrolyzate, containing reducing sugars supplemented with a minimum of (NH4)2SO4, MnSO4, and yeast extract, was used as moistening agent to impregnate 5g of RS, which was used as the inert support for SSF. Maximum L-lactic acid production of 3.467g per 5g of support was obtained at 37 oC, using Lactobacillus casei as inoculum, after 5 days of fermentation with optimized process parameters such as 72% moisture content, 4g per 5g support of reducing sugars, 2.5ml per 5g support of inoculum size, 3g per 5g support of CaCO3, and pH 6.5.http://ojs.cnr.ncsu.edu/index.php/BioRes/article/viewFile/BioRes_2_3_419_429_Qi_Yao_LacticAcid_RiceStraw/58L-lactic acidSolid state fermentationRice strawHydrolysateLactobacillus casei
spellingShingle Risheng, Yao
Benkun Qi
L-lactic acid production from Lactobacillus casei by solid state fermentation using rice straw
BioResources
L-lactic acid
Solid state fermentation
Rice straw
Hydrolysate
Lactobacillus casei
title L-lactic acid production from Lactobacillus casei by solid state fermentation using rice straw
title_full L-lactic acid production from Lactobacillus casei by solid state fermentation using rice straw
title_fullStr L-lactic acid production from Lactobacillus casei by solid state fermentation using rice straw
title_full_unstemmed L-lactic acid production from Lactobacillus casei by solid state fermentation using rice straw
title_short L-lactic acid production from Lactobacillus casei by solid state fermentation using rice straw
title_sort l lactic acid production from lactobacillus casei by solid state fermentation using rice straw
topic L-lactic acid
Solid state fermentation
Rice straw
Hydrolysate
Lactobacillus casei
url http://ojs.cnr.ncsu.edu/index.php/BioRes/article/viewFile/BioRes_2_3_419_429_Qi_Yao_LacticAcid_RiceStraw/58
work_keys_str_mv AT rishengyao llacticacidproductionfromlactobacilluscaseibysolidstatefermentationusingricestraw
AT benkunqi llacticacidproductionfromlactobacilluscaseibysolidstatefermentationusingricestraw