L-lactic acid production from Lactobacillus casei by solid state fermentation using rice straw
In order to make full use of rice straw (RS) produced at large quantity in China and to reduce the production cost of L-lactic acid, attempts were made to utilize the hydrolysate of RS as sole carbon source and the lignocellulose as inert support for producing L-lactic acid using solid state ferment...
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North Carolina State University
2007-01-01
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Series: | BioResources |
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Online Access: | http://ojs.cnr.ncsu.edu/index.php/BioRes/article/viewFile/BioRes_2_3_419_429_Qi_Yao_LacticAcid_RiceStraw/58 |
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author | Risheng, Yao Benkun Qi |
author_facet | Risheng, Yao Benkun Qi |
author_sort | Risheng, Yao |
collection | DOAJ |
description | In order to make full use of rice straw (RS) produced at large quantity in China and to reduce the production cost of L-lactic acid, attempts were made to utilize the hydrolysate of RS as sole carbon source and the lignocellulose as inert support for producing L-lactic acid using solid state fermentation (SSF). The pretreated rice straw was enzymatically hydro- lyzed by cellulase, and the hydrolyzate, containing reducing sugars supplemented with a minimum of (NH4)2SO4, MnSO4, and yeast extract, was used as moistening agent to impregnate 5g of RS, which was used as the inert support for SSF. Maximum L-lactic acid production of 3.467g per 5g of support was obtained at 37 oC, using Lactobacillus casei as inoculum, after 5 days of fermentation with optimized process parameters such as 72% moisture content, 4g per 5g support of reducing sugars, 2.5ml per 5g support of inoculum size, 3g per 5g support of CaCO3, and pH 6.5. |
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issn | 1930-2126 |
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spelling | doaj.art-27453563de8f41dca4dfc531c6cc0ff82022-12-21T18:11:14ZengNorth Carolina State UniversityBioResources1930-21262007-01-0123419429L-lactic acid production from Lactobacillus casei by solid state fermentation using rice strawRisheng, YaoBenkun QiIn order to make full use of rice straw (RS) produced at large quantity in China and to reduce the production cost of L-lactic acid, attempts were made to utilize the hydrolysate of RS as sole carbon source and the lignocellulose as inert support for producing L-lactic acid using solid state fermentation (SSF). The pretreated rice straw was enzymatically hydro- lyzed by cellulase, and the hydrolyzate, containing reducing sugars supplemented with a minimum of (NH4)2SO4, MnSO4, and yeast extract, was used as moistening agent to impregnate 5g of RS, which was used as the inert support for SSF. Maximum L-lactic acid production of 3.467g per 5g of support was obtained at 37 oC, using Lactobacillus casei as inoculum, after 5 days of fermentation with optimized process parameters such as 72% moisture content, 4g per 5g support of reducing sugars, 2.5ml per 5g support of inoculum size, 3g per 5g support of CaCO3, and pH 6.5.http://ojs.cnr.ncsu.edu/index.php/BioRes/article/viewFile/BioRes_2_3_419_429_Qi_Yao_LacticAcid_RiceStraw/58L-lactic acidSolid state fermentationRice strawHydrolysateLactobacillus casei |
spellingShingle | Risheng, Yao Benkun Qi L-lactic acid production from Lactobacillus casei by solid state fermentation using rice straw BioResources L-lactic acid Solid state fermentation Rice straw Hydrolysate Lactobacillus casei |
title | L-lactic acid production from Lactobacillus casei by solid state fermentation using rice straw |
title_full | L-lactic acid production from Lactobacillus casei by solid state fermentation using rice straw |
title_fullStr | L-lactic acid production from Lactobacillus casei by solid state fermentation using rice straw |
title_full_unstemmed | L-lactic acid production from Lactobacillus casei by solid state fermentation using rice straw |
title_short | L-lactic acid production from Lactobacillus casei by solid state fermentation using rice straw |
title_sort | l lactic acid production from lactobacillus casei by solid state fermentation using rice straw |
topic | L-lactic acid Solid state fermentation Rice straw Hydrolysate Lactobacillus casei |
url | http://ojs.cnr.ncsu.edu/index.php/BioRes/article/viewFile/BioRes_2_3_419_429_Qi_Yao_LacticAcid_RiceStraw/58 |
work_keys_str_mv | AT rishengyao llacticacidproductionfromlactobacilluscaseibysolidstatefermentationusingricestraw AT benkunqi llacticacidproductionfromlactobacilluscaseibysolidstatefermentationusingricestraw |