Preparation, Characterization, and Biological Properties of Hydroxyapatite from Bigeye Snapper (<i>Priancanthus tayenus</i>) Bone
The optimum condition of acid hydrolysis for hydroxyapatite extraction from bigeye snapper (<i>Priancanthus tayenus</i>) bone and the effects of extraction time (10–60 min) and HCl concentration (2.0–5.0% <i>w</i>/<i>v</i>) on yield and hydroxyapatite properties w...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-02-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/3/2776 |
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| author | Nunnuth Jindapon Phatthranit Klinmalai Utoomporn Surayot Nuttapol Tanadchangsaeng Woradej Pichaiaukrit Yuthana Phimolsiripol Chaluntorn Vichasilp Sutee Wangtueai |
| author_facet | Nunnuth Jindapon Phatthranit Klinmalai Utoomporn Surayot Nuttapol Tanadchangsaeng Woradej Pichaiaukrit Yuthana Phimolsiripol Chaluntorn Vichasilp Sutee Wangtueai |
| author_sort | Nunnuth Jindapon |
| collection | DOAJ |
| description | The optimum condition of acid hydrolysis for hydroxyapatite extraction from bigeye snapper (<i>Priancanthus tayenus</i>) bone and the effects of extraction time (10–60 min) and HCl concentration (2.0–5.0% <i>w</i>/<i>v</i>) on yield and hydroxyapatite properties were determined. The optimum extracted condition was found using 5% HCl for 60 min, which was 13.4% yield; 19.8 g/100 g Ca content; 9.6 g/100 g P content; 2.1 Ca/P ratio; <i>L</i>*, <i>a</i>*, <i>b</i>*; and Δ<i>E</i> as 84.5, 2.8, 16.5, and 15.6, respectively. The using of 5% NaOH solution was optimum for hydroxyapatite precipitation from the extracted solution. The characteristic and biological properties of the obtained hydroxyapatite were studied. Fourier transform infrared spectroscopy and X-ray diffraction results showed a good comparison between the extracted and commercial hydroxyapatite. The microstructure of the extracted hydroxyapatite from a scanning electron microscope showed an irregular and flat-plate shape, large surface area, and roughness. The extracted hydroxyapatite was non- and low-cytotoxicity at a concentration of 50 and 100–400 µg/mL, respectively. Bovine serum albumin (BSA) adsorption and desorption of hydroxyapatite was studied. An increasing BSA concentration, hydroxyapatite amount, and adsorption time significantly increased protein adsorption on hydroxyapatite. Protein desorption from BSA-loaded hydroxyapatite showed an increase of release initially in the first 4 days and became a steady release rate until 14 days. |
| first_indexed | 2024-03-11T09:39:37Z |
| format | Article |
| id | doaj.art-274a048058b6420f999c96b169dd069d |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T09:39:37Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-274a048058b6420f999c96b169dd069d2023-11-16T17:02:48ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-02-01243277610.3390/ijms24032776Preparation, Characterization, and Biological Properties of Hydroxyapatite from Bigeye Snapper (<i>Priancanthus tayenus</i>) BoneNunnuth Jindapon0Phatthranit Klinmalai1Utoomporn Surayot2Nuttapol Tanadchangsaeng3Woradej Pichaiaukrit4Yuthana Phimolsiripol5Chaluntorn Vichasilp6Sutee Wangtueai7College of Maritime Studies and Management, Chiang Mai University, Samut Sakhon 74000, ThailandCollege of Maritime Studies and Management, Chiang Mai University, Samut Sakhon 74000, ThailandCollege of Maritime Studies and Management, Chiang Mai University, Samut Sakhon 74000, ThailandCollege of Biomedical Engineering, Rangsit University, Pathumthani 12000, ThailandCollege of Dental Medicine, Rangsit University, Pathumthani 12000, ThailandFaculty of Agro-Industry, Chiang Mai University, Chiang Mai 50100, ThailandFaculty of Natural Resources, Rajamangala University of Technology Isan, Sakon Nakhon 47160, ThailandCollege of Maritime Studies and Management, Chiang Mai University, Samut Sakhon 74000, ThailandThe optimum condition of acid hydrolysis for hydroxyapatite extraction from bigeye snapper (<i>Priancanthus tayenus</i>) bone and the effects of extraction time (10–60 min) and HCl concentration (2.0–5.0% <i>w</i>/<i>v</i>) on yield and hydroxyapatite properties were determined. The optimum extracted condition was found using 5% HCl for 60 min, which was 13.4% yield; 19.8 g/100 g Ca content; 9.6 g/100 g P content; 2.1 Ca/P ratio; <i>L</i>*, <i>a</i>*, <i>b</i>*; and Δ<i>E</i> as 84.5, 2.8, 16.5, and 15.6, respectively. The using of 5% NaOH solution was optimum for hydroxyapatite precipitation from the extracted solution. The characteristic and biological properties of the obtained hydroxyapatite were studied. Fourier transform infrared spectroscopy and X-ray diffraction results showed a good comparison between the extracted and commercial hydroxyapatite. The microstructure of the extracted hydroxyapatite from a scanning electron microscope showed an irregular and flat-plate shape, large surface area, and roughness. The extracted hydroxyapatite was non- and low-cytotoxicity at a concentration of 50 and 100–400 µg/mL, respectively. Bovine serum albumin (BSA) adsorption and desorption of hydroxyapatite was studied. An increasing BSA concentration, hydroxyapatite amount, and adsorption time significantly increased protein adsorption on hydroxyapatite. Protein desorption from BSA-loaded hydroxyapatite showed an increase of release initially in the first 4 days and became a steady release rate until 14 days.https://www.mdpi.com/1422-0067/24/3/2776hydroxyapatitebigeye snapper<i>Priancanthus tayenus</i>fish boneprotein adsorptionprotein desorption |
| spellingShingle | Nunnuth Jindapon Phatthranit Klinmalai Utoomporn Surayot Nuttapol Tanadchangsaeng Woradej Pichaiaukrit Yuthana Phimolsiripol Chaluntorn Vichasilp Sutee Wangtueai Preparation, Characterization, and Biological Properties of Hydroxyapatite from Bigeye Snapper (<i>Priancanthus tayenus</i>) Bone International Journal of Molecular Sciences hydroxyapatite bigeye snapper <i>Priancanthus tayenus</i> fish bone protein adsorption protein desorption |
| title | Preparation, Characterization, and Biological Properties of Hydroxyapatite from Bigeye Snapper (<i>Priancanthus tayenus</i>) Bone |
| title_full | Preparation, Characterization, and Biological Properties of Hydroxyapatite from Bigeye Snapper (<i>Priancanthus tayenus</i>) Bone |
| title_fullStr | Preparation, Characterization, and Biological Properties of Hydroxyapatite from Bigeye Snapper (<i>Priancanthus tayenus</i>) Bone |
| title_full_unstemmed | Preparation, Characterization, and Biological Properties of Hydroxyapatite from Bigeye Snapper (<i>Priancanthus tayenus</i>) Bone |
| title_short | Preparation, Characterization, and Biological Properties of Hydroxyapatite from Bigeye Snapper (<i>Priancanthus tayenus</i>) Bone |
| title_sort | preparation characterization and biological properties of hydroxyapatite from bigeye snapper i priancanthus tayenus i bone |
| topic | hydroxyapatite bigeye snapper <i>Priancanthus tayenus</i> fish bone protein adsorption protein desorption |
| url | https://www.mdpi.com/1422-0067/24/3/2776 |
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