Primary and secondary hyperparathyroidism present different expressions of calcium-sensing receptor
Abstract Background Decreased calcium-sensing receptor (CaSR) has been observed in hyperparathyroidism (HPT) without a known mechanism. The purpose of this study was to evaluate the expression of CaSR in primary (PHPT) and secondary (SHPT) subtypes. Methods Immunohistochemical (IHC) staining and qua...
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| Format: | Article |
| Language: | English |
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BMC
2023-02-01
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| Series: | BMC Surgery |
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| Online Access: | https://doi.org/10.1186/s12893-023-01928-5 |
| _version_ | 1811166046829150208 |
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| author | Xin Li Yao Lu Ling Zhang Aiping Song Honglei Zhang Bo Pang Jun Liu Xiaoliang Sun Haoyang Ji Linping Huang Meng Yang |
| author_facet | Xin Li Yao Lu Ling Zhang Aiping Song Honglei Zhang Bo Pang Jun Liu Xiaoliang Sun Haoyang Ji Linping Huang Meng Yang |
| author_sort | Xin Li |
| collection | DOAJ |
| description | Abstract Background Decreased calcium-sensing receptor (CaSR) has been observed in hyperparathyroidism (HPT) without a known mechanism. The purpose of this study was to evaluate the expression of CaSR in primary (PHPT) and secondary (SHPT) subtypes. Methods Immunohistochemical (IHC) staining and quantitative real-time PCR (qRT-PCR) assay were used to measure the differences in expression of CaSR protein and gene in PHPT and SHPT human samples, compared to matched controls. Results CaSR protein was differentially downregulated in SHPT and PHPT compared to normal parathyroid tissues (2.42 ± 0.5 vs. 3.2 ± 0.62, P < 0.05; 1.8 ± 0.83 vs. 3.2 ± 0.62, P < 0.05, respectively). Furthermore, SHPT tissues exhibited significantly higher levels of CaSR mRNA (0.29 ± 0.23 vs. 0.01 ± 0.12, P < 0.05) and protein (2.42 ± 0.5 vs. 1.8 ± 0.83, P < 0.05) than those in PHPT tissue samples. Conclusion Depressed CaSR expression was a critical pathological hallmark of HPT. We found a differential decline of CaSR, in terms of both mRNA and protein levels, in PHPT and SHPT human samples. We think that CaSR dysregulation occurred at the very beginning of disease onset in PHPT, while a similar pathological scenario appeared at the later stage of SHPT. Future studies should be directed to dissect the mechanistic involvement of CaSR in PHPT and SHPT in order to bring treatment precisions in HPT management. |
| first_indexed | 2024-04-10T15:46:05Z |
| format | Article |
| id | doaj.art-274afad519e04588a32e086b4be11da0 |
| institution | Directory Open Access Journal |
| issn | 1471-2482 |
| language | English |
| last_indexed | 2024-04-10T15:46:05Z |
| publishDate | 2023-02-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Surgery |
| spelling | doaj.art-274afad519e04588a32e086b4be11da02023-02-12T12:03:29ZengBMCBMC Surgery1471-24822023-02-012311510.1186/s12893-023-01928-5Primary and secondary hyperparathyroidism present different expressions of calcium-sensing receptorXin Li0Yao Lu1Ling Zhang2Aiping Song3Honglei Zhang4Bo Pang5Jun Liu6Xiaoliang Sun7Haoyang Ji8Linping Huang9Meng Yang10Institute of Clinical Medicine Research, China-Japan Friendship HospitalDepartment of General Surgery, China-Japan Friendship HospitalCenter of Nephrology, China-Japan Friendship HospitalDepartment of Pathology, China-Japan Friendship HospitalDepartment of Pathology, China-Japan Friendship HospitalState Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, China CDC 155Department of General Surgery, China-Japan Friendship HospitalDepartment of General Surgery, China-Japan Friendship HospitalDepartment of General Surgery, China-Japan Friendship HospitalDepartment of General Surgery, China-Japan Friendship HospitalDepartment of General Surgery, China-Japan Friendship HospitalAbstract Background Decreased calcium-sensing receptor (CaSR) has been observed in hyperparathyroidism (HPT) without a known mechanism. The purpose of this study was to evaluate the expression of CaSR in primary (PHPT) and secondary (SHPT) subtypes. Methods Immunohistochemical (IHC) staining and quantitative real-time PCR (qRT-PCR) assay were used to measure the differences in expression of CaSR protein and gene in PHPT and SHPT human samples, compared to matched controls. Results CaSR protein was differentially downregulated in SHPT and PHPT compared to normal parathyroid tissues (2.42 ± 0.5 vs. 3.2 ± 0.62, P < 0.05; 1.8 ± 0.83 vs. 3.2 ± 0.62, P < 0.05, respectively). Furthermore, SHPT tissues exhibited significantly higher levels of CaSR mRNA (0.29 ± 0.23 vs. 0.01 ± 0.12, P < 0.05) and protein (2.42 ± 0.5 vs. 1.8 ± 0.83, P < 0.05) than those in PHPT tissue samples. Conclusion Depressed CaSR expression was a critical pathological hallmark of HPT. We found a differential decline of CaSR, in terms of both mRNA and protein levels, in PHPT and SHPT human samples. We think that CaSR dysregulation occurred at the very beginning of disease onset in PHPT, while a similar pathological scenario appeared at the later stage of SHPT. Future studies should be directed to dissect the mechanistic involvement of CaSR in PHPT and SHPT in order to bring treatment precisions in HPT management.https://doi.org/10.1186/s12893-023-01928-5Calcium-sensing receptorSecondary hyperparathyroidismPrimary hyperparathyroidism |
| spellingShingle | Xin Li Yao Lu Ling Zhang Aiping Song Honglei Zhang Bo Pang Jun Liu Xiaoliang Sun Haoyang Ji Linping Huang Meng Yang Primary and secondary hyperparathyroidism present different expressions of calcium-sensing receptor BMC Surgery Calcium-sensing receptor Secondary hyperparathyroidism Primary hyperparathyroidism |
| title | Primary and secondary hyperparathyroidism present different expressions of calcium-sensing receptor |
| title_full | Primary and secondary hyperparathyroidism present different expressions of calcium-sensing receptor |
| title_fullStr | Primary and secondary hyperparathyroidism present different expressions of calcium-sensing receptor |
| title_full_unstemmed | Primary and secondary hyperparathyroidism present different expressions of calcium-sensing receptor |
| title_short | Primary and secondary hyperparathyroidism present different expressions of calcium-sensing receptor |
| title_sort | primary and secondary hyperparathyroidism present different expressions of calcium sensing receptor |
| topic | Calcium-sensing receptor Secondary hyperparathyroidism Primary hyperparathyroidism |
| url | https://doi.org/10.1186/s12893-023-01928-5 |
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