Measuring and modeling macrophage proliferation in a lab-on-CMOS capacitance sensing microsystem

Measuring and modeling macrophage proliferation in a lab-on-CMOS capacitance sensing microsystem

We report on the use of a lab-on-CMOS biosensor platform for quantitatively tracking the proliferation of RAW 264.7 murine Balb/c macrophages. We show that macrophage proliferation correlates linearly with an average capacitance growth factor resulting from capacitance measurements at a plurality of...

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Bibliographic Details
Main Authors: Kyle Smith, Ching-Yi Lin, Yann Gilpin, Elizabeth Wayne, Marc Dandin
Format: Article
Language:English
Published: Frontiers Media S.A. 2023-05-01
Series:Frontiers in Bioengineering and Biotechnology
Subjects:
CMOS
biosensors
capacitance
lab-on-chip
lab-on-CMOS
model
Online Access:https://www.frontiersin.org/articles/10.3389/fbioe.2023.1159004/full
Description
Summary:We report on the use of a lab-on-CMOS biosensor platform for quantitatively tracking the proliferation of RAW 264.7 murine Balb/c macrophages. We show that macrophage proliferation correlates linearly with an average capacitance growth factor resulting from capacitance measurements at a plurality of electrodes dispersed in a sensing area of interest. We further show a temporal model that captures the cell number evolution in the area over long periods (e.g., 30 h). The model links the cell numbers and the average capacitance growth factor to describe the observed cell proliferation.
ISSN:2296-4185

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