Measuring and modeling macrophage proliferation in a lab-on-CMOS capacitance sensing microsystem
We report on the use of a lab-on-CMOS biosensor platform for quantitatively tracking the proliferation of RAW 264.7 murine Balb/c macrophages. We show that macrophage proliferation correlates linearly with an average capacitance growth factor resulting from capacitance measurements at a plurality of...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-05-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2023.1159004/full |
| _version_ | 1797828916860157952 |
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| author | Kyle Smith Ching-Yi Lin Yann Gilpin Elizabeth Wayne Elizabeth Wayne Marc Dandin Marc Dandin |
| author_facet | Kyle Smith Ching-Yi Lin Yann Gilpin Elizabeth Wayne Elizabeth Wayne Marc Dandin Marc Dandin |
| author_sort | Kyle Smith |
| collection | DOAJ |
| description | We report on the use of a lab-on-CMOS biosensor platform for quantitatively tracking the proliferation of RAW 264.7 murine Balb/c macrophages. We show that macrophage proliferation correlates linearly with an average capacitance growth factor resulting from capacitance measurements at a plurality of electrodes dispersed in a sensing area of interest. We further show a temporal model that captures the cell number evolution in the area over long periods (e.g., 30 h). The model links the cell numbers and the average capacitance growth factor to describe the observed cell proliferation. |
| first_indexed | 2024-04-09T13:12:04Z |
| format | Article |
| id | doaj.art-2758630dc74944c494fbe0a629d5300b |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-04-09T13:12:04Z |
| publishDate | 2023-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-2758630dc74944c494fbe0a629d5300b2023-05-12T06:29:21ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852023-05-011110.3389/fbioe.2023.11590041159004Measuring and modeling macrophage proliferation in a lab-on-CMOS capacitance sensing microsystemKyle Smith0Ching-Yi Lin1Yann Gilpin2Elizabeth Wayne3Elizabeth Wayne4Marc Dandin5Marc Dandin6Department of Chemical Engineering, Pittsburgh, PA, United StatesDepartment of Electrical and Computer Engineering, Pittsburgh, PA, United StatesDepartment of Electrical and Computer Engineering, Pittsburgh, PA, United StatesDepartment of Chemical Engineering, Pittsburgh, PA, United StatesDepartment of Biomedical Engineering, Carnegie Mellon University, Pittsburgh, PA, United StatesDepartment of Electrical and Computer Engineering, Pittsburgh, PA, United StatesDepartment of Biomedical Engineering, Carnegie Mellon University, Pittsburgh, PA, United StatesWe report on the use of a lab-on-CMOS biosensor platform for quantitatively tracking the proliferation of RAW 264.7 murine Balb/c macrophages. We show that macrophage proliferation correlates linearly with an average capacitance growth factor resulting from capacitance measurements at a plurality of electrodes dispersed in a sensing area of interest. We further show a temporal model that captures the cell number evolution in the area over long periods (e.g., 30 h). The model links the cell numbers and the average capacitance growth factor to describe the observed cell proliferation.https://www.frontiersin.org/articles/10.3389/fbioe.2023.1159004/fullCMOSbiosensorscapacitancelab-on-chiplab-on-CMOSmodel |
| spellingShingle | Kyle Smith Ching-Yi Lin Yann Gilpin Elizabeth Wayne Elizabeth Wayne Marc Dandin Marc Dandin Measuring and modeling macrophage proliferation in a lab-on-CMOS capacitance sensing microsystem Frontiers in Bioengineering and Biotechnology CMOS biosensors capacitance lab-on-chip lab-on-CMOS model |
| title | Measuring and modeling macrophage proliferation in a lab-on-CMOS capacitance sensing microsystem |
| title_full | Measuring and modeling macrophage proliferation in a lab-on-CMOS capacitance sensing microsystem |
| title_fullStr | Measuring and modeling macrophage proliferation in a lab-on-CMOS capacitance sensing microsystem |
| title_full_unstemmed | Measuring and modeling macrophage proliferation in a lab-on-CMOS capacitance sensing microsystem |
| title_short | Measuring and modeling macrophage proliferation in a lab-on-CMOS capacitance sensing microsystem |
| title_sort | measuring and modeling macrophage proliferation in a lab on cmos capacitance sensing microsystem |
| topic | CMOS biosensors capacitance lab-on-chip lab-on-CMOS model |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2023.1159004/full |
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