Reliability Evaluation of Fluorescence In Situ Hybridization (FISH) and G-Banding on Bone Marrow and Peripheral Blood Cells in Chronic Myelogenous Leukemia Patients
Chronic myeloid leukemia (CML) is a myeloproliferative disease. The cytogenetic hallmark of CML is Philadelphia (Ph) chromosome. This study aimed to diagnose suspected CML patients, to monitor CML patients under therapy using cytogenetic and fluorescence in situ hybridization (FISH) techniques to...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Royan Institute (ACECR), Tehran
2015-04-01
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| Series: | Cell Journal |
| Subjects: | |
| Online Access: | http://celljournal.org/library/upload/article/af_4323348484748323225528584526682363647622Cell-J-17-171-.pdf |
| Summary: | Chronic myeloid leukemia (CML) is a myeloproliferative disease. The cytogenetic hallmark
of CML is Philadelphia (Ph) chromosome. This study aimed to diagnose suspected CML
patients, to monitor CML patients under therapy using cytogenetic and fluorescence in
situ hybridization (FISH) techniques to analyze their bone marrow (BM) and peripheral
blood (PB) samples, and finally to compare their obtained results for both specimens. This
study was conducted during one-year period (2012-2013). The participants were recruited
from the Hematology and Oncology Clinic of Shahid Gazi (Emam Reza) Hospital of Tabriz
University of Medical Sciences, Tabriz, East Azerbaijan Province, Iran. We analyzed 90
samples from 60 suspected CML patients (30 BM and 60 PB samples). All samples were
analyzed using G-banding, 5 samples using dual fusion FISH (DF-FISH) probes, as well
as 30 samples using both FISH and G-banding. Among the 90 analyzed samples of 60 patients,
25 (41.66%) were Ph+ using karyotyping, whereas five cases were not analyzable,
so FISH was applied and the results confirmed that only two individuals were BCR-ABL+.
In the comparison between 25 BM and 25 PB samples using karyotyping, 15 (60%) and
10 (40%) were ph+, respectively. The comparison of FISH and karyotyping on 30 samples
showed that 9 (30%) and 8 (26.66%) were Ph+, respectively, and only 18.18% of Ph+
patients showed atypical patterns. In the comparison between BM-cytogenetic and PBinterphase-
FISH (I-FISH), BM-cytogenetic was more reliable than PB-I-FISH in detecting
Ph. Our data demonstrate that FISH analysis is a rapid, reliable and sensitive technique.
The comparison between BM and PB showed that PB can not be replaced by BM, even
in detecting by FISH. |
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| ISSN: | 2228-5806 2228-5814 |