Supragingival microbiome alternations as a consequence of smoking different tobacco types and its relation to dental caries
Abstract This study aimed to assess the effect of smoking different tobacco types on the supragingival microbiome and its relation to dental caries. Forty supragingival plaque samples were collected from smokers of a single tobacco type and non-smokers seeking treatment at the University Dental Hosp...
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| Format: | Article |
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Nature Portfolio
2022-02-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-06907-z |
| _version_ | 1818501251802857472 |
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| author | Farah Al-Marzooq Sausan Al Kawas Betul Rahman Jenni A. Shearston Hiba Saad Dalenda Benzina Michael Weitzman |
| author_facet | Farah Al-Marzooq Sausan Al Kawas Betul Rahman Jenni A. Shearston Hiba Saad Dalenda Benzina Michael Weitzman |
| author_sort | Farah Al-Marzooq |
| collection | DOAJ |
| description | Abstract This study aimed to assess the effect of smoking different tobacco types on the supragingival microbiome and its relation to dental caries. Forty supragingival plaque samples were collected from smokers of a single tobacco type and non-smokers seeking treatment at the University Dental Hospital Sharjah, UAE. DMFT (decayed, missing and filled teeth) was determined for all participants who were divided into two groups: no-low caries (NC-LC: DMFT = 0–4; n = 18) and moderate-high caries (MC-HC: DMFT = 5–20; n = 22). 16S rRNA gene was sequenced using third-generation sequencing with Nanopore technology. Microbiome composition and diversity were compared. Caries was most common among cigarette smokers. Supragingival microbiota were significantly altered among smokers of different tobacco types. In cigarette smokers, cariogenic bacteria from genus Streptococcus (including S. mutans) were significantly more among subjects with NC-LC, while Lactobacilli (including L. fermentum) were more among subjects with MC-HC. In medwakh smokers, several periodontopathogens were significantly elevated in subjects with NC-LC, while other pathogenic bacteria (as Klebsiella pneumoniae) were more in those with MC-HC. Cigarette and alternative tobacco smoking had a significant impact on the supragingival microbiome. Indeed, further studies are required to unravel the consequences of oral dysbiosis triggered by smoking. This could pave the way for microbiota-based interventional measures for restoring a healthy oral microbiome which could be a promising strategy to prevent dental caries. |
| first_indexed | 2024-12-10T20:53:38Z |
| format | Article |
| id | doaj.art-275b8b9009c541f4b10c56145e26b9cf |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-10T20:53:38Z |
| publishDate | 2022-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-275b8b9009c541f4b10c56145e26b9cf2022-12-22T01:34:01ZengNature PortfolioScientific Reports2045-23222022-02-0112111410.1038/s41598-022-06907-zSupragingival microbiome alternations as a consequence of smoking different tobacco types and its relation to dental cariesFarah Al-Marzooq0Sausan Al Kawas1Betul Rahman2Jenni A. Shearston3Hiba Saad4Dalenda Benzina5Michael Weitzman6Department of Medical Microbiology and Immunology, College of Medicine and Health Sciences, UAE UniversityDepartment of Oral and Craniofacial Health Sciences, College of Dental Medicine, University of SharjahSharjah Institute for Medical Research, University of SharjahDepartment of Environmental Health Sciences, Mailman School of Public Health, Columbia UniversityDepartment of Oral and Craniofacial Health Sciences, College of Dental Medicine, University of SharjahDepartment of Oral and Craniofacial Health Sciences, College of Dental Medicine, University of SharjahDepartment of Pediatrics, School of Medicine, New York UniversityAbstract This study aimed to assess the effect of smoking different tobacco types on the supragingival microbiome and its relation to dental caries. Forty supragingival plaque samples were collected from smokers of a single tobacco type and non-smokers seeking treatment at the University Dental Hospital Sharjah, UAE. DMFT (decayed, missing and filled teeth) was determined for all participants who were divided into two groups: no-low caries (NC-LC: DMFT = 0–4; n = 18) and moderate-high caries (MC-HC: DMFT = 5–20; n = 22). 16S rRNA gene was sequenced using third-generation sequencing with Nanopore technology. Microbiome composition and diversity were compared. Caries was most common among cigarette smokers. Supragingival microbiota were significantly altered among smokers of different tobacco types. In cigarette smokers, cariogenic bacteria from genus Streptococcus (including S. mutans) were significantly more among subjects with NC-LC, while Lactobacilli (including L. fermentum) were more among subjects with MC-HC. In medwakh smokers, several periodontopathogens were significantly elevated in subjects with NC-LC, while other pathogenic bacteria (as Klebsiella pneumoniae) were more in those with MC-HC. Cigarette and alternative tobacco smoking had a significant impact on the supragingival microbiome. Indeed, further studies are required to unravel the consequences of oral dysbiosis triggered by smoking. This could pave the way for microbiota-based interventional measures for restoring a healthy oral microbiome which could be a promising strategy to prevent dental caries.https://doi.org/10.1038/s41598-022-06907-z |
| spellingShingle | Farah Al-Marzooq Sausan Al Kawas Betul Rahman Jenni A. Shearston Hiba Saad Dalenda Benzina Michael Weitzman Supragingival microbiome alternations as a consequence of smoking different tobacco types and its relation to dental caries Scientific Reports |
| title | Supragingival microbiome alternations as a consequence of smoking different tobacco types and its relation to dental caries |
| title_full | Supragingival microbiome alternations as a consequence of smoking different tobacco types and its relation to dental caries |
| title_fullStr | Supragingival microbiome alternations as a consequence of smoking different tobacco types and its relation to dental caries |
| title_full_unstemmed | Supragingival microbiome alternations as a consequence of smoking different tobacco types and its relation to dental caries |
| title_short | Supragingival microbiome alternations as a consequence of smoking different tobacco types and its relation to dental caries |
| title_sort | supragingival microbiome alternations as a consequence of smoking different tobacco types and its relation to dental caries |
| url | https://doi.org/10.1038/s41598-022-06907-z |
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