Development of a versatile laboratory experiment to teach the metabolic transformation of hydrolysis
In this paper we describe an easy, reliable, versatile and inexpensive laboratory experiment to teach the metabolic transformation of hydrolysis to Pharmacy students. The experiment does not require the sacrifice of any experimental animal, or any work with organs or tissues, and so can be implement...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
University of Huddersfield Press
2016-11-01
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| Series: | British Journal of Pharmacy |
| Online Access: | https://www.bjpharm.org.uk/article/id/13/ |
| _version_ | 1797816713973071872 |
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| author | Antonella Campanile Kim Morral Mohammad Khaled Aljammal Felix Owusu-Kwarteng Mohammed Shabbir Ian Beadham Jordi Morral |
| author_facet | Antonella Campanile Kim Morral Mohammad Khaled Aljammal Felix Owusu-Kwarteng Mohammed Shabbir Ian Beadham Jordi Morral |
| author_sort | Antonella Campanile |
| collection | DOAJ |
| description | In this paper we describe an easy, reliable, versatile and inexpensive laboratory experiment to teach the metabolic transformation of hydrolysis to Pharmacy students. The experiment does not require the sacrifice of any experimental animal, or any work with organs or tissues, and so can be implemented in a typical university chemistry laboratory. We used acetylsalicylic acid (ASA), hexyl salicylate (HS) and two enzymes, a lipase and an esterase. Since both ASS and HS liberate salicylic acid (SA) upon hydrolysis, students can evaluate the different enzymatic transformations by monitoring the amount of SA liberated. The learning outcomes are an enhanced student understanding of: (1) the process of hydrolysis; (2) the application of enzymatic transformations of molecules from food to xenobiotics; (3) the differences between the general specificity of substrate of both enzymes; (4) the concepts of the lipophilic pocket; (5) the catalytic triad and its regioselectivity in relation to the ester bond. A questionnaire was administered to participating students at three points in time: at the beginning of the module, after enzymatic hydrolysis was taught in class, and after the laboratory experiment. From an analysis of the questionnaire data we conclude that this practical helped Pharmacy students to understand these concepts. |
| first_indexed | 2024-03-13T08:42:51Z |
| format | Article |
| id | doaj.art-275cf28c05984688a68458489809705f |
| institution | Directory Open Access Journal |
| issn | 2058-8356 |
| language | English |
| last_indexed | 2024-03-13T08:42:51Z |
| publishDate | 2016-11-01 |
| publisher | University of Huddersfield Press |
| record_format | Article |
| series | British Journal of Pharmacy |
| spelling | doaj.art-275cf28c05984688a68458489809705f2023-05-30T08:35:45ZengUniversity of Huddersfield PressBritish Journal of Pharmacy2058-83562016-11-011110.5920/bjpharm.2016.12Development of a versatile laboratory experiment to teach the metabolic transformation of hydrolysisAntonella Campanile0Kim Morral1Mohammad Khaled Aljammal2Felix Owusu-Kwarteng3Mohammed Shabbir4Ian Beadham5Jordi Morral 6University of HuddersfieldQualitas ResearchUniversity of HuddersfieldUniversity of HuddersfieldUniversity of HuddersfieldKingston UniversityUniversity of HuddersfieldIn this paper we describe an easy, reliable, versatile and inexpensive laboratory experiment to teach the metabolic transformation of hydrolysis to Pharmacy students. The experiment does not require the sacrifice of any experimental animal, or any work with organs or tissues, and so can be implemented in a typical university chemistry laboratory. We used acetylsalicylic acid (ASA), hexyl salicylate (HS) and two enzymes, a lipase and an esterase. Since both ASS and HS liberate salicylic acid (SA) upon hydrolysis, students can evaluate the different enzymatic transformations by monitoring the amount of SA liberated. The learning outcomes are an enhanced student understanding of: (1) the process of hydrolysis; (2) the application of enzymatic transformations of molecules from food to xenobiotics; (3) the differences between the general specificity of substrate of both enzymes; (4) the concepts of the lipophilic pocket; (5) the catalytic triad and its regioselectivity in relation to the ester bond. A questionnaire was administered to participating students at three points in time: at the beginning of the module, after enzymatic hydrolysis was taught in class, and after the laboratory experiment. From an analysis of the questionnaire data we conclude that this practical helped Pharmacy students to understand these concepts.https://www.bjpharm.org.uk/article/id/13/ |
| spellingShingle | Antonella Campanile Kim Morral Mohammad Khaled Aljammal Felix Owusu-Kwarteng Mohammed Shabbir Ian Beadham Jordi Morral Development of a versatile laboratory experiment to teach the metabolic transformation of hydrolysis British Journal of Pharmacy |
| title | Development of a versatile laboratory experiment to teach the metabolic transformation of hydrolysis |
| title_full | Development of a versatile laboratory experiment to teach the metabolic transformation of hydrolysis |
| title_fullStr | Development of a versatile laboratory experiment to teach the metabolic transformation of hydrolysis |
| title_full_unstemmed | Development of a versatile laboratory experiment to teach the metabolic transformation of hydrolysis |
| title_short | Development of a versatile laboratory experiment to teach the metabolic transformation of hydrolysis |
| title_sort | development of a versatile laboratory experiment to teach the metabolic transformation of hydrolysis |
| url | https://www.bjpharm.org.uk/article/id/13/ |
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