Effects of Hydroxy-Alpha-Sanshool on Intestinal Metabolism in Insulin-Resistant Mice
To explore the hydroxy-alpha-sanshool (HAS) effects on the intestinal metabolites of insulin-resistant mice, the blank group (BG), model group (MG), and HAS dose group (DG) were designed. The insulin resistance (IR) model was induced through streptozotocin (STZ) combined with a high-fat and high-sug...
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| Format: | Article |
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MDPI AG
2022-07-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/11/14/2040 |
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| author | Fangyan Xu Yuping Zhu Mintao Lu Likang Qin Degang Zhao Tingyuan Ren |
| author_facet | Fangyan Xu Yuping Zhu Mintao Lu Likang Qin Degang Zhao Tingyuan Ren |
| author_sort | Fangyan Xu |
| collection | DOAJ |
| description | To explore the hydroxy-alpha-sanshool (HAS) effects on the intestinal metabolites of insulin-resistant mice, the blank group (BG), model group (MG), and HAS dose group (DG) were designed. The insulin resistance (IR) model was induced through streptozotocin (STZ) combined with a high-fat and high-sugar diet. Based on the availability of the model, the HAS dose was given by gavage for 28 days. The determination of cecum and key serum indexes was made, including the contents of insulin (INS), triglycerides (TG), total cholesterol (TC), glycosylated serum protein (GSP), and glycosylated hemoglobin (GHb). The changes in gut microbiota and metabolites in cecal contents were detected by 16S rRNA gene amplicon sequencing and UPLC/HRMS technology, respectively. The results that the levels of GSP, GHb, TG, and TC were significantly increased; this was not the case for INS; or for the changes in the gut microbiota and metabolites in MG. However, the intervention of HAS effectively reversed these changes, for instance, it decreased levels of GSP, GHb, TG, TC, and alterations of metabolite composition for linoleic acid and tyrosine metabolism and recovered trends of declining species diversity and richness of the gut microbiota in MG. It was indicated that HAS alleviated IR by regulating the gut microbiota and metabolites and affecting lipid and amino acid metabolism pathways. |
| first_indexed | 2024-03-09T03:26:10Z |
| format | Article |
| id | doaj.art-27633cba8fa4459794cdcca1331935ce |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-09T03:26:10Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-27633cba8fa4459794cdcca1331935ce2023-12-03T15:02:34ZengMDPI AGFoods2304-81582022-07-011114204010.3390/foods11142040Effects of Hydroxy-Alpha-Sanshool on Intestinal Metabolism in Insulin-Resistant MiceFangyan Xu0Yuping Zhu1Mintao Lu2Likang Qin3Degang Zhao4Tingyuan Ren5College of Brewing and Food Engineering, Guizhou University, Guiyang 550025, ChinaSchool of Basic Medicine, Guizhou Medical University, Guiyang 550025, ChinaCollege of Brewing and Food Engineering, Guizhou University, Guiyang 550025, ChinaCollege of Brewing and Food Engineering, Guizhou University, Guiyang 550025, ChinaCollege of Brewing and Food Engineering, Guizhou University, Guiyang 550025, ChinaCollege of Brewing and Food Engineering, Guizhou University, Guiyang 550025, ChinaTo explore the hydroxy-alpha-sanshool (HAS) effects on the intestinal metabolites of insulin-resistant mice, the blank group (BG), model group (MG), and HAS dose group (DG) were designed. The insulin resistance (IR) model was induced through streptozotocin (STZ) combined with a high-fat and high-sugar diet. Based on the availability of the model, the HAS dose was given by gavage for 28 days. The determination of cecum and key serum indexes was made, including the contents of insulin (INS), triglycerides (TG), total cholesterol (TC), glycosylated serum protein (GSP), and glycosylated hemoglobin (GHb). The changes in gut microbiota and metabolites in cecal contents were detected by 16S rRNA gene amplicon sequencing and UPLC/HRMS technology, respectively. The results that the levels of GSP, GHb, TG, and TC were significantly increased; this was not the case for INS; or for the changes in the gut microbiota and metabolites in MG. However, the intervention of HAS effectively reversed these changes, for instance, it decreased levels of GSP, GHb, TG, TC, and alterations of metabolite composition for linoleic acid and tyrosine metabolism and recovered trends of declining species diversity and richness of the gut microbiota in MG. It was indicated that HAS alleviated IR by regulating the gut microbiota and metabolites and affecting lipid and amino acid metabolism pathways.https://www.mdpi.com/2304-8158/11/14/2040hydroxy-alpha-sanshoolinsulin resistantmetabolismintestines |
| spellingShingle | Fangyan Xu Yuping Zhu Mintao Lu Likang Qin Degang Zhao Tingyuan Ren Effects of Hydroxy-Alpha-Sanshool on Intestinal Metabolism in Insulin-Resistant Mice Foods hydroxy-alpha-sanshool insulin resistant metabolism intestines |
| title | Effects of Hydroxy-Alpha-Sanshool on Intestinal Metabolism in Insulin-Resistant Mice |
| title_full | Effects of Hydroxy-Alpha-Sanshool on Intestinal Metabolism in Insulin-Resistant Mice |
| title_fullStr | Effects of Hydroxy-Alpha-Sanshool on Intestinal Metabolism in Insulin-Resistant Mice |
| title_full_unstemmed | Effects of Hydroxy-Alpha-Sanshool on Intestinal Metabolism in Insulin-Resistant Mice |
| title_short | Effects of Hydroxy-Alpha-Sanshool on Intestinal Metabolism in Insulin-Resistant Mice |
| title_sort | effects of hydroxy alpha sanshool on intestinal metabolism in insulin resistant mice |
| topic | hydroxy-alpha-sanshool insulin resistant metabolism intestines |
| url | https://www.mdpi.com/2304-8158/11/14/2040 |
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