Comprehensive Analysis of the Expression and Clinical Significance of THO Complex Members in Hepatocellular Carcinoma
Xixi Li,1 Zefeng Liu,1 Xin Wei,1 Jie Lin,1 Qiwei Yang,2 Yingjun Xie1 1Department of Hepatobiliary and Pancreatic Surgery, The Second Hospital of Jilin University, Changchun, People’s Republic of China; 2Medical Research Center, The Second Hospital of Jilin University, Changchun, People’s Republic of...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2022-03-01
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| Series: | International Journal of General Medicine |
| Subjects: | |
| Online Access: | https://www.dovepress.com/comprehensive-analysis-of-the-expression-and-clinical-significance-of--peer-reviewed-fulltext-article-IJGM |
| _version_ | 1819294765521305600 |
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| author | Li X Liu Z Wei X Lin J Yang Q Xie Y |
| author_facet | Li X Liu Z Wei X Lin J Yang Q Xie Y |
| author_sort | Li X |
| collection | DOAJ |
| description | Xixi Li,1 Zefeng Liu,1 Xin Wei,1 Jie Lin,1 Qiwei Yang,2 Yingjun Xie1 1Department of Hepatobiliary and Pancreatic Surgery, The Second Hospital of Jilin University, Changchun, People’s Republic of China; 2Medical Research Center, The Second Hospital of Jilin University, Changchun, People’s Republic of ChinaCorrespondence: Yingjun Xie, Tel +86 17390069233, Email xiezz54@jlu.edu.cnBackground: THO complex members (THOCs) are essential for mRNA processing, transcription elongation, mRNA export and genome integrity, which may be associated with tumor. However, the significance of THO complex members in Hepatocellular carcinoma (HCC) has not been clarified.Methods: In this study, we investigated expression levels, clinicopathological correlation, diagnostic and prognostic value, disease associations, co-expression network, functional enrichment, genetic variation, drug sensitivity, molecular targets, and immune infiltration of THOCs in HCC using UALCAN, ICGC, HPA, Kaplan–Meier Plotter, The Open Targets Platform database, GeneMANIA, WebGestalt, GSCALite, LinkedOmics, TIMER. Meanwhile, the mRNA expression levels of THOCs in HCC were verified by qRT-PCR.Results: THOCs were significantly up-regulated at the transcription level in HCC, and overexpression of THOCs was correlated with clinicopathological features. THOCs had potential diagnostic and prognostic value for HCC. THOCs could participate in apoptosis, regulate cell cycle, and were also involved in DNA damage response. Important cancer-related signaling pathways such as EMT, RAS/MAPK pathway and RKT pathway were also related to THOCs expression. In addition, the expression level of THOC1/2/6/7 was negatively correlated with drug resistance. And miRNA, kinase, and transcription factor targets associated with THOCs have been identified. Meanwhile, THOCs were associated with immune infiltration in HCC.Conclusion: Our study revealed that THO complex members have important clinical significance for HCC and have potential clinical utility in diagnosis, treatment and prognosis.Keywords: HCC, THOC, bioinformatics, prognosis, diagnosis |
| first_indexed | 2024-12-24T04:31:31Z |
| format | Article |
| id | doaj.art-2765942e562540a9b4feb17fd8257f87 |
| institution | Directory Open Access Journal |
| issn | 1178-7074 |
| language | English |
| last_indexed | 2024-12-24T04:31:31Z |
| publishDate | 2022-03-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | International Journal of General Medicine |
| spelling | doaj.art-2765942e562540a9b4feb17fd8257f872022-12-21T17:15:23ZengDove Medical PressInternational Journal of General Medicine1178-70742022-03-01Volume 152695271373540Comprehensive Analysis of the Expression and Clinical Significance of THO Complex Members in Hepatocellular CarcinomaLi XLiu ZWei XLin JYang QXie YXixi Li,1 Zefeng Liu,1 Xin Wei,1 Jie Lin,1 Qiwei Yang,2 Yingjun Xie1 1Department of Hepatobiliary and Pancreatic Surgery, The Second Hospital of Jilin University, Changchun, People’s Republic of China; 2Medical Research Center, The Second Hospital of Jilin University, Changchun, People’s Republic of ChinaCorrespondence: Yingjun Xie, Tel +86 17390069233, Email xiezz54@jlu.edu.cnBackground: THO complex members (THOCs) are essential for mRNA processing, transcription elongation, mRNA export and genome integrity, which may be associated with tumor. However, the significance of THO complex members in Hepatocellular carcinoma (HCC) has not been clarified.Methods: In this study, we investigated expression levels, clinicopathological correlation, diagnostic and prognostic value, disease associations, co-expression network, functional enrichment, genetic variation, drug sensitivity, molecular targets, and immune infiltration of THOCs in HCC using UALCAN, ICGC, HPA, Kaplan–Meier Plotter, The Open Targets Platform database, GeneMANIA, WebGestalt, GSCALite, LinkedOmics, TIMER. Meanwhile, the mRNA expression levels of THOCs in HCC were verified by qRT-PCR.Results: THOCs were significantly up-regulated at the transcription level in HCC, and overexpression of THOCs was correlated with clinicopathological features. THOCs had potential diagnostic and prognostic value for HCC. THOCs could participate in apoptosis, regulate cell cycle, and were also involved in DNA damage response. Important cancer-related signaling pathways such as EMT, RAS/MAPK pathway and RKT pathway were also related to THOCs expression. In addition, the expression level of THOC1/2/6/7 was negatively correlated with drug resistance. And miRNA, kinase, and transcription factor targets associated with THOCs have been identified. Meanwhile, THOCs were associated with immune infiltration in HCC.Conclusion: Our study revealed that THO complex members have important clinical significance for HCC and have potential clinical utility in diagnosis, treatment and prognosis.Keywords: HCC, THOC, bioinformatics, prognosis, diagnosishttps://www.dovepress.com/comprehensive-analysis-of-the-expression-and-clinical-significance-of--peer-reviewed-fulltext-article-IJGMhcctho complex(thoc)bioinformaticsprognosisdiagnosis |
| spellingShingle | Li X Liu Z Wei X Lin J Yang Q Xie Y Comprehensive Analysis of the Expression and Clinical Significance of THO Complex Members in Hepatocellular Carcinoma International Journal of General Medicine hcc tho complex(thoc) bioinformatics prognosis diagnosis |
| title | Comprehensive Analysis of the Expression and Clinical Significance of THO Complex Members in Hepatocellular Carcinoma |
| title_full | Comprehensive Analysis of the Expression and Clinical Significance of THO Complex Members in Hepatocellular Carcinoma |
| title_fullStr | Comprehensive Analysis of the Expression and Clinical Significance of THO Complex Members in Hepatocellular Carcinoma |
| title_full_unstemmed | Comprehensive Analysis of the Expression and Clinical Significance of THO Complex Members in Hepatocellular Carcinoma |
| title_short | Comprehensive Analysis of the Expression and Clinical Significance of THO Complex Members in Hepatocellular Carcinoma |
| title_sort | comprehensive analysis of the expression and clinical significance of tho complex members in hepatocellular carcinoma |
| topic | hcc tho complex(thoc) bioinformatics prognosis diagnosis |
| url | https://www.dovepress.com/comprehensive-analysis-of-the-expression-and-clinical-significance-of--peer-reviewed-fulltext-article-IJGM |
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