A Novel In Vitro Simulator to Investigate Promotion of Reconstruction of Damaged Neuronal Cell Colony Differentiated from iPS Cells with the Aid of Micro Dynamic Stimulation
Neuronal cells are equipped with the function of a sensor that senses stimulation and elongates neurites to connect nearby neuronal cells in forming a neuronal network, as they are generally said to be hard to recover from physical damage, such as in the case of a spinal cord injury. Therefore, in t...
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MDPI AG
2021-11-01
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Online Access: | https://www.mdpi.com/2227-7080/9/4/83 |
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author | Tadashi Kosawada Taku Kitsunai Zhonggang Feng Kaoru Goto |
author_facet | Tadashi Kosawada Taku Kitsunai Zhonggang Feng Kaoru Goto |
author_sort | Tadashi Kosawada |
collection | DOAJ |
description | Neuronal cells are equipped with the function of a sensor that senses stimulation and elongates neurites to connect nearby neuronal cells in forming a neuronal network, as they are generally said to be hard to recover from physical damage, such as in the case of a spinal cord injury. Therefore, in this study, a novel in vitro simulator in which micro dynamic stimulations are applied to a damaged neuronal cell colony artificially is proposed to investigate the possibility of promoting the reconstruction of damaged neuronal cells on a colony basis. A neuronal cell colony differentiated from iPS cells is physically damaged by cutting off treatment, and micro dynamic stimulations are applied to the colony by utilizing a developed mini-vibration table system. NeuroFluor NeuO is used to establish a method for fluorescent staining of the living neuronal cells, and morphologies of the reconstructing neurons are analysed, revealing a relationship between the stimulation and the reconstructing process of the damaged neurons. It is found that significant differences are observed in the reconstructing efficiency between the statically cultured damaged neuronal cell colony and the dynamically stimulated one. The results suggest that applying appropriate micro dynamic stimulations is a promising approach to promote the reconstruction of a damaged neuronal cell colony. |
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issn | 2227-7080 |
language | English |
last_indexed | 2024-03-10T03:58:44Z |
publishDate | 2021-11-01 |
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spelling | doaj.art-276a2b58a8c2409aad62c1cde874a01d2023-11-23T10:48:08ZengMDPI AGTechnologies2227-70802021-11-01948310.3390/technologies9040083A Novel In Vitro Simulator to Investigate Promotion of Reconstruction of Damaged Neuronal Cell Colony Differentiated from iPS Cells with the Aid of Micro Dynamic StimulationTadashi Kosawada0Taku Kitsunai1Zhonggang Feng2Kaoru Goto3Graduate School of Science and Engineering, Yamagata University, 4-3-16 Jonan, Yonezawa 992-8510, Yamagata, JapanGraduate School of Science and Engineering, Yamagata University, 4-3-16 Jonan, Yonezawa 992-8510, Yamagata, JapanGraduate School of Science and Engineering, Yamagata University, 4-3-16 Jonan, Yonezawa 992-8510, Yamagata, JapanSchool of Medicine, Yamagata University, 2-2-2 Iida-nishi, Yamagata 990-9585, Yamagata, JapanNeuronal cells are equipped with the function of a sensor that senses stimulation and elongates neurites to connect nearby neuronal cells in forming a neuronal network, as they are generally said to be hard to recover from physical damage, such as in the case of a spinal cord injury. Therefore, in this study, a novel in vitro simulator in which micro dynamic stimulations are applied to a damaged neuronal cell colony artificially is proposed to investigate the possibility of promoting the reconstruction of damaged neuronal cells on a colony basis. A neuronal cell colony differentiated from iPS cells is physically damaged by cutting off treatment, and micro dynamic stimulations are applied to the colony by utilizing a developed mini-vibration table system. NeuroFluor NeuO is used to establish a method for fluorescent staining of the living neuronal cells, and morphologies of the reconstructing neurons are analysed, revealing a relationship between the stimulation and the reconstructing process of the damaged neurons. It is found that significant differences are observed in the reconstructing efficiency between the statically cultured damaged neuronal cell colony and the dynamically stimulated one. The results suggest that applying appropriate micro dynamic stimulations is a promising approach to promote the reconstruction of a damaged neuronal cell colony.https://www.mdpi.com/2227-7080/9/4/83micro dynamic stimulationmini-vibration tablein vitro simulatoriPS cellneuronal cell colonyphysical damage |
spellingShingle | Tadashi Kosawada Taku Kitsunai Zhonggang Feng Kaoru Goto A Novel In Vitro Simulator to Investigate Promotion of Reconstruction of Damaged Neuronal Cell Colony Differentiated from iPS Cells with the Aid of Micro Dynamic Stimulation Technologies micro dynamic stimulation mini-vibration table in vitro simulator iPS cell neuronal cell colony physical damage |
title | A Novel In Vitro Simulator to Investigate Promotion of Reconstruction of Damaged Neuronal Cell Colony Differentiated from iPS Cells with the Aid of Micro Dynamic Stimulation |
title_full | A Novel In Vitro Simulator to Investigate Promotion of Reconstruction of Damaged Neuronal Cell Colony Differentiated from iPS Cells with the Aid of Micro Dynamic Stimulation |
title_fullStr | A Novel In Vitro Simulator to Investigate Promotion of Reconstruction of Damaged Neuronal Cell Colony Differentiated from iPS Cells with the Aid of Micro Dynamic Stimulation |
title_full_unstemmed | A Novel In Vitro Simulator to Investigate Promotion of Reconstruction of Damaged Neuronal Cell Colony Differentiated from iPS Cells with the Aid of Micro Dynamic Stimulation |
title_short | A Novel In Vitro Simulator to Investigate Promotion of Reconstruction of Damaged Neuronal Cell Colony Differentiated from iPS Cells with the Aid of Micro Dynamic Stimulation |
title_sort | novel in vitro simulator to investigate promotion of reconstruction of damaged neuronal cell colony differentiated from ips cells with the aid of micro dynamic stimulation |
topic | micro dynamic stimulation mini-vibration table in vitro simulator iPS cell neuronal cell colony physical damage |
url | https://www.mdpi.com/2227-7080/9/4/83 |
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