An interlaboratory comparison of ITS2-PCR for the identification of yeasts, using the ABI Prism 310 and CEQ8000 capillary electrophoresis systems
<p>Abstract</p> <p>Background</p> <p>Currently, most laboratories identify yeasts routinely on the basis of morphology and biochemical reactivity. This approach has quite often limited discriminatory power and may require long incubation periods. Due to the increase of...
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| Format: | Article |
| Language: | English |
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BMC
2005-03-01
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| Series: | BMC Microbiology |
| Online Access: | http://www.biomedcentral.com/1471-2180/5/14 |
| _version_ | 1818755545338740736 |
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| author | Verschraegen Gerda Boel An De Beenhouwer Hans Van Hauwe Peter Van Keerberghen Anne De Baere Thierry Claeys Geert Vaneechoutte Mario |
| author_facet | Verschraegen Gerda Boel An De Beenhouwer Hans Van Hauwe Peter Van Keerberghen Anne De Baere Thierry Claeys Geert Vaneechoutte Mario |
| author_sort | Verschraegen Gerda |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Currently, most laboratories identify yeasts routinely on the basis of morphology and biochemical reactivity. This approach has quite often limited discriminatory power and may require long incubation periods. Due to the increase of fungal infections and due to specific antifungal resistence patterns for different species, accurate and rapid identification has become more important. Several molecular techniques have been described for fast and reliable identification of yeast isolates, but interlaboratory exchangeability of identification schemes of molecular techniques has hardly been studied. Here, we compared amplified ITS2 fragment length determination by an ABI Prism 310 (Applied Biosystems, Foster City, Ca.) capillary electrophoresis system with that obtained by a CEQ8000 (Beckman Coulter, Fullerton, Ca.) capillary electrophoresis system.</p> <p>Results</p> <p>Although ITS2 size estimations on both systems differed and separate libraries had to be constructed for each system, both approaches had the same discriminatory power with regard to the 44 reference strains, identical identifications were obtained for 39/ 40 clinical isolates in both laboratories and strains from 51 samples were correctly identified using CEQ8000, when compared to phenotypic identification.</p> <p>Conclusion</p> <p>Identification of yeasts with ITS2-PCR followed by fragment analysis can be carried out on different capillary electrophoresis systems with comparable discriminatory power.</p> |
| first_indexed | 2024-12-18T05:40:51Z |
| format | Article |
| id | doaj.art-276d5e3c76de4b00af1b7946f34ded87 |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-12-18T05:40:51Z |
| publishDate | 2005-03-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-276d5e3c76de4b00af1b7946f34ded872022-12-21T21:19:10ZengBMCBMC Microbiology1471-21802005-03-01511410.1186/1471-2180-5-14An interlaboratory comparison of ITS2-PCR for the identification of yeasts, using the ABI Prism 310 and CEQ8000 capillary electrophoresis systemsVerschraegen GerdaBoel AnDe Beenhouwer HansVan Hauwe PeterVan Keerberghen AnneDe Baere ThierryClaeys GeertVaneechoutte Mario<p>Abstract</p> <p>Background</p> <p>Currently, most laboratories identify yeasts routinely on the basis of morphology and biochemical reactivity. This approach has quite often limited discriminatory power and may require long incubation periods. Due to the increase of fungal infections and due to specific antifungal resistence patterns for different species, accurate and rapid identification has become more important. Several molecular techniques have been described for fast and reliable identification of yeast isolates, but interlaboratory exchangeability of identification schemes of molecular techniques has hardly been studied. Here, we compared amplified ITS2 fragment length determination by an ABI Prism 310 (Applied Biosystems, Foster City, Ca.) capillary electrophoresis system with that obtained by a CEQ8000 (Beckman Coulter, Fullerton, Ca.) capillary electrophoresis system.</p> <p>Results</p> <p>Although ITS2 size estimations on both systems differed and separate libraries had to be constructed for each system, both approaches had the same discriminatory power with regard to the 44 reference strains, identical identifications were obtained for 39/ 40 clinical isolates in both laboratories and strains from 51 samples were correctly identified using CEQ8000, when compared to phenotypic identification.</p> <p>Conclusion</p> <p>Identification of yeasts with ITS2-PCR followed by fragment analysis can be carried out on different capillary electrophoresis systems with comparable discriminatory power.</p>http://www.biomedcentral.com/1471-2180/5/14 |
| spellingShingle | Verschraegen Gerda Boel An De Beenhouwer Hans Van Hauwe Peter Van Keerberghen Anne De Baere Thierry Claeys Geert Vaneechoutte Mario An interlaboratory comparison of ITS2-PCR for the identification of yeasts, using the ABI Prism 310 and CEQ8000 capillary electrophoresis systems BMC Microbiology |
| title | An interlaboratory comparison of ITS2-PCR for the identification of yeasts, using the ABI Prism 310 and CEQ8000 capillary electrophoresis systems |
| title_full | An interlaboratory comparison of ITS2-PCR for the identification of yeasts, using the ABI Prism 310 and CEQ8000 capillary electrophoresis systems |
| title_fullStr | An interlaboratory comparison of ITS2-PCR for the identification of yeasts, using the ABI Prism 310 and CEQ8000 capillary electrophoresis systems |
| title_full_unstemmed | An interlaboratory comparison of ITS2-PCR for the identification of yeasts, using the ABI Prism 310 and CEQ8000 capillary electrophoresis systems |
| title_short | An interlaboratory comparison of ITS2-PCR for the identification of yeasts, using the ABI Prism 310 and CEQ8000 capillary electrophoresis systems |
| title_sort | interlaboratory comparison of its2 pcr for the identification of yeasts using the abi prism 310 and ceq8000 capillary electrophoresis systems |
| url | http://www.biomedcentral.com/1471-2180/5/14 |
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