Integration and characterization of T-DNA insertion in upland cotton
Copy numbers were evaluated by real-time quantitative PCR, and 149 junctions of T-DNA were isolated by thermal asymmetric interlaced PCR from 92 independent transgenic cotton lines transformed by Agrobacterium tumefaciens strain LBA4404. Real-time quantitative PCR results showed that 46% had integra...
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| Format: | Article |
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Czech Academy of Agricultural Sciences
2013-06-01
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| Series: | Czech Journal of Genetics and Plant Breeding |
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| Online Access: | https://cjgpb.agriculturejournals.cz/artkey/cjg-201302-0001_integration-and-characterization-of-t-dna-insertion-in-upland-cotton.php |
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| author | Xiaojie YANG Fuguang LI Xueyan ZHANG Kun LIU Qianhua WANG Chaojun ZHANG Chuanliang LIU Wei ZHU Guofang SHAN Chee-Kok CHIN Weiping FANG |
| author_facet | Xiaojie YANG Fuguang LI Xueyan ZHANG Kun LIU Qianhua WANG Chaojun ZHANG Chuanliang LIU Wei ZHU Guofang SHAN Chee-Kok CHIN Weiping FANG |
| author_sort | Xiaojie YANG |
| collection | DOAJ |
| description | Copy numbers were evaluated by real-time quantitative PCR, and 149 junctions of T-DNA were isolated by thermal asymmetric interlaced PCR from 92 independent transgenic cotton lines transformed by Agrobacterium tumefaciens strain LBA4404. Real-time quantitative PCR results showed that 46% had integration of one or two T-DNA copies, 54% had three or more copies. Among 63 amplified products at LB junctions, 51% showed co-transformation of the vector backbone, 30% retained a portion of LB ranging from 3 to 23 bp, and 19% showed deletions ranging from 1 to 148 bp from the LB inner end. In contrast, all of the cleavage sites were located in the inner region of RB. The distribution of T-DNA insertions in upland cotton genome included coding sequences, transposons, plastid-derived sequences and microsatellites. |
| first_indexed | 2024-04-10T08:29:09Z |
| format | Article |
| id | doaj.art-276e42bfce2f4a75ac8932a789ad4dd8 |
| institution | Directory Open Access Journal |
| issn | 1212-1975 1805-9325 |
| language | English |
| last_indexed | 2024-04-10T08:29:09Z |
| publishDate | 2013-06-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Czech Journal of Genetics and Plant Breeding |
| spelling | doaj.art-276e42bfce2f4a75ac8932a789ad4dd82023-02-23T03:30:18ZengCzech Academy of Agricultural SciencesCzech Journal of Genetics and Plant Breeding1212-19751805-93252013-06-01492515710.17221/217/2012-CJGPBcjg-201302-0001Integration and characterization of T-DNA insertion in upland cottonXiaojie YANG0Fuguang LI1Xueyan ZHANG2Kun LIU3Qianhua WANG4Chaojun ZHANG5Chuanliang LIU6Wei ZHU7Guofang SHAN8Chee-Kok CHIN9Weiping FANG10State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agriculture Sciences (CAAS), Anyang, Henan, P.R. ChinaState Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agriculture Sciences (CAAS), Anyang, Henan, P.R. ChinaState Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agriculture Sciences (CAAS), Anyang, Henan, P.R. ChinaState Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agriculture Sciences (CAAS), Anyang, Henan, P.R. ChinaState Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agriculture Sciences (CAAS), Anyang, Henan, P.R. ChinaState Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agriculture Sciences (CAAS), Anyang, Henan, P.R. ChinaState Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agriculture Sciences (CAAS), Anyang, Henan, P.R. ChinaAgronomy College of Henan Agricultural University, Zhengzhou, P.R. ChinaState Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agriculture Sciences (CAAS), Anyang, Henan, P.R. ChinaDepartment of Plant Science, Rutgers University, New Jersey, USAEconomic Crop Research Institute, Henan Academy of Agricultural Sciences, Zhengzhou, P.R. ChinaCopy numbers were evaluated by real-time quantitative PCR, and 149 junctions of T-DNA were isolated by thermal asymmetric interlaced PCR from 92 independent transgenic cotton lines transformed by Agrobacterium tumefaciens strain LBA4404. Real-time quantitative PCR results showed that 46% had integration of one or two T-DNA copies, 54% had three or more copies. Among 63 amplified products at LB junctions, 51% showed co-transformation of the vector backbone, 30% retained a portion of LB ranging from 3 to 23 bp, and 19% showed deletions ranging from 1 to 148 bp from the LB inner end. In contrast, all of the cleavage sites were located in the inner region of RB. The distribution of T-DNA insertions in upland cotton genome included coding sequences, transposons, plastid-derived sequences and microsatellites.https://cjgpb.agriculturejournals.cz/artkey/cjg-201302-0001_integration-and-characterization-of-t-dna-insertion-in-upland-cotton.phpcotton (gossypium hirsutum l.)deletion of bordergenetic transformationtransgene copyvector integration |
| spellingShingle | Xiaojie YANG Fuguang LI Xueyan ZHANG Kun LIU Qianhua WANG Chaojun ZHANG Chuanliang LIU Wei ZHU Guofang SHAN Chee-Kok CHIN Weiping FANG Integration and characterization of T-DNA insertion in upland cotton Czech Journal of Genetics and Plant Breeding cotton (gossypium hirsutum l.) deletion of border genetic transformation transgene copy vector integration |
| title | Integration and characterization of T-DNA insertion in upland cotton |
| title_full | Integration and characterization of T-DNA insertion in upland cotton |
| title_fullStr | Integration and characterization of T-DNA insertion in upland cotton |
| title_full_unstemmed | Integration and characterization of T-DNA insertion in upland cotton |
| title_short | Integration and characterization of T-DNA insertion in upland cotton |
| title_sort | integration and characterization of t dna insertion in upland cotton |
| topic | cotton (gossypium hirsutum l.) deletion of border genetic transformation transgene copy vector integration |
| url | https://cjgpb.agriculturejournals.cz/artkey/cjg-201302-0001_integration-and-characterization-of-t-dna-insertion-in-upland-cotton.php |
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