Integration and characterization of T-DNA insertion in upland cotton

Integration and characterization of T-DNA insertion in upland cotton

Copy numbers were evaluated by real-time quantitative PCR, and 149 junctions of T-DNA were isolated by thermal asymmetric interlaced PCR from 92 independent transgenic cotton lines transformed by Agrobacterium tumefaciens strain LBA4404. Real-time quantitative PCR results showed that 46% had integra...

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Bibliographic Details
Main Authors: Xiaojie YANG, Fuguang LI, Xueyan ZHANG, Kun LIU, Qianhua WANG, Chaojun ZHANG, Chuanliang LIU, Wei ZHU, Guofang SHAN, Chee-Kok CHIN, Weiping FANG
Format: Article
Language:English
Published: Czech Academy of Agricultural Sciences 2013-06-01
Series:Czech Journal of Genetics and Plant Breeding
Subjects:
cotton (gossypium hirsutum l.)
deletion of border
genetic transformation
transgene copy
vector integration
Online Access:https://cjgpb.agriculturejournals.cz/artkey/cjg-201302-0001_integration-and-characterization-of-t-dna-insertion-in-upland-cotton.php

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https://cjgpb.agriculturejournals.cz/artkey/cjg-201302-0001_integration-and-characterization-of-t-dna-insertion-in-upland-cotton.php

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