Integration and characterization of T-DNA insertion in upland cotton
Copy numbers were evaluated by real-time quantitative PCR, and 149 junctions of T-DNA were isolated by thermal asymmetric interlaced PCR from 92 independent transgenic cotton lines transformed by Agrobacterium tumefaciens strain LBA4404. Real-time quantitative PCR results showed that 46% had integra...
Main Authors: | Xiaojie YANG, Fuguang LI, Xueyan ZHANG, Kun LIU, Qianhua WANG, Chaojun ZHANG, Chuanliang LIU, Wei ZHU, Guofang SHAN, Chee-Kok CHIN, Weiping FANG |
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Format: | Article |
Language: | English |
Published: |
Czech Academy of Agricultural Sciences
2013-06-01
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Series: | Czech Journal of Genetics and Plant Breeding |
Subjects: | |
Online Access: | https://cjgpb.agriculturejournals.cz/artkey/cjg-201302-0001_integration-and-characterization-of-t-dna-insertion-in-upland-cotton.php |
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