Preliminary Study of Resistance Mechanism of <i>Botrytis cinerea</i> to SYAUP-CN-26
SYAUP-CN-26 (1<i>S</i>, 2<i>R</i>-((3-bromophenethyl)amino)-<i>N</i>-(4-chloro-2-trifluoromethylphenyl) cyclohexane-1-sulfonamide) is a novel sulfonamide compound with excellent activity against <i>Botrytis cinerea</i>. The present study sought to expl...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-01-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/27/3/936 |
| _version_ | 1827659699863945216 |
|---|---|
| author | Kai Wang Huazhong Zhang Wanying Zhu Jingnan Peng Xinghai Li Yingzi Wang Zhiqiu Qi |
| author_facet | Kai Wang Huazhong Zhang Wanying Zhu Jingnan Peng Xinghai Li Yingzi Wang Zhiqiu Qi |
| author_sort | Kai Wang |
| collection | DOAJ |
| description | SYAUP-CN-26 (1<i>S</i>, 2<i>R</i>-((3-bromophenethyl)amino)-<i>N</i>-(4-chloro-2-trifluoromethylphenyl) cyclohexane-1-sulfonamide) is a novel sulfonamide compound with excellent activity against <i>Botrytis cinerea</i>. The present study sought to explore the mutant of <i>B.</i><i>cinerea</i> resistant to SYAUP-CN-26 using SYAUP-CN-26 plates. Moreover, the cell membrane functions of <i>B.</i><i>cinerea</i>, histidine kinase activity, relative conductivity, triglyceride, and cell membrane structure were determined, and the target gene histidine kinase Bos1 (AF396827.2) of procymidone was amplified and sequenced. The results showed that compared to the sensitive strain, the cell membrane permeability, triglyceride, and histidine kinase activity of the resistant strain showed significant changes. The relative conductivity of the sensitive strain increased by 6.95% and 9.61%, while the relative conductivity of the resistant strain increased by 0.23% and 1.76% with 26.785 µg/mL (EC<sub>95</sub>) and 79.754 µg/mL (MIC) of SYAUP-CN-26 treatment. The triglyceride inhibition rate of the resistant strain was 23.49% and 37.80%, which was 0.23% and 1.76% higher than the sensitive strain. Compared to the sensitive strain, the histidine kinase activity of the resistant strain was increased by 23.07% and 35.61%, respectively. SYAUP-CN-26 significantly damaged the cell membrane structure of the sensitive strain. The sequencing of the Bos1 gene of the sensitive and resistant strains indicated that SYAUP-CN-26 resistance was associated with a single point mutation (P348L) in the Bos1 gene. Therefore, it was inferred that the mutant of <i>B.</i><i>cinerea</i> resistant to SYAUP-CN-26 might be regulated by the Bos1 gene. This study will provide a theoretical basis for further research and development of sulfonamide compounds for <i>B. cinerea</i> and new agents for the prevention and control of resistant <i>B. cinerea</i>. |
| first_indexed | 2024-03-09T23:28:01Z |
| format | Article |
| id | doaj.art-276f0af3615c4c76815d18383be1aa4b |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T23:28:01Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-276f0af3615c4c76815d18383be1aa4b2023-11-23T17:14:54ZengMDPI AGMolecules1420-30492022-01-0127393610.3390/molecules27030936Preliminary Study of Resistance Mechanism of <i>Botrytis cinerea</i> to SYAUP-CN-26Kai Wang0Huazhong Zhang1Wanying Zhu2Jingnan Peng3Xinghai Li4Yingzi Wang5Zhiqiu Qi6Department of Pesticide Science, Plant Protection College, Shenyang Agricultural University, Shenyang 110866, ChinaDepartment of Pesticide Science, Plant Protection College, Shenyang Agricultural University, Shenyang 110866, ChinaDepartment of Pesticide Science, Plant Protection College, Shenyang Agricultural University, Shenyang 110866, ChinaDepartment of Pesticide Science, Plant Protection College, Shenyang Agricultural University, Shenyang 110866, ChinaDepartment of Pesticide Science, Plant Protection College, Shenyang Agricultural University, Shenyang 110866, ChinaDepartment of Pesticide Science, Plant Protection College, Shenyang Agricultural University, Shenyang 110866, ChinaDepartment of Pesticide Science, Plant Protection College, Shenyang Agricultural University, Shenyang 110866, ChinaSYAUP-CN-26 (1<i>S</i>, 2<i>R</i>-((3-bromophenethyl)amino)-<i>N</i>-(4-chloro-2-trifluoromethylphenyl) cyclohexane-1-sulfonamide) is a novel sulfonamide compound with excellent activity against <i>Botrytis cinerea</i>. The present study sought to explore the mutant of <i>B.</i><i>cinerea</i> resistant to SYAUP-CN-26 using SYAUP-CN-26 plates. Moreover, the cell membrane functions of <i>B.</i><i>cinerea</i>, histidine kinase activity, relative conductivity, triglyceride, and cell membrane structure were determined, and the target gene histidine kinase Bos1 (AF396827.2) of procymidone was amplified and sequenced. The results showed that compared to the sensitive strain, the cell membrane permeability, triglyceride, and histidine kinase activity of the resistant strain showed significant changes. The relative conductivity of the sensitive strain increased by 6.95% and 9.61%, while the relative conductivity of the resistant strain increased by 0.23% and 1.76% with 26.785 µg/mL (EC<sub>95</sub>) and 79.754 µg/mL (MIC) of SYAUP-CN-26 treatment. The triglyceride inhibition rate of the resistant strain was 23.49% and 37.80%, which was 0.23% and 1.76% higher than the sensitive strain. Compared to the sensitive strain, the histidine kinase activity of the resistant strain was increased by 23.07% and 35.61%, respectively. SYAUP-CN-26 significantly damaged the cell membrane structure of the sensitive strain. The sequencing of the Bos1 gene of the sensitive and resistant strains indicated that SYAUP-CN-26 resistance was associated with a single point mutation (P348L) in the Bos1 gene. Therefore, it was inferred that the mutant of <i>B.</i><i>cinerea</i> resistant to SYAUP-CN-26 might be regulated by the Bos1 gene. This study will provide a theoretical basis for further research and development of sulfonamide compounds for <i>B. cinerea</i> and new agents for the prevention and control of resistant <i>B. cinerea</i>.https://www.mdpi.com/1420-3049/27/3/936SYAUP-CN-26<i>Botrytis cinerea</i>activitycell membrane |
| spellingShingle | Kai Wang Huazhong Zhang Wanying Zhu Jingnan Peng Xinghai Li Yingzi Wang Zhiqiu Qi Preliminary Study of Resistance Mechanism of <i>Botrytis cinerea</i> to SYAUP-CN-26 Molecules SYAUP-CN-26 <i>Botrytis cinerea</i> activity cell membrane |
| title | Preliminary Study of Resistance Mechanism of <i>Botrytis cinerea</i> to SYAUP-CN-26 |
| title_full | Preliminary Study of Resistance Mechanism of <i>Botrytis cinerea</i> to SYAUP-CN-26 |
| title_fullStr | Preliminary Study of Resistance Mechanism of <i>Botrytis cinerea</i> to SYAUP-CN-26 |
| title_full_unstemmed | Preliminary Study of Resistance Mechanism of <i>Botrytis cinerea</i> to SYAUP-CN-26 |
| title_short | Preliminary Study of Resistance Mechanism of <i>Botrytis cinerea</i> to SYAUP-CN-26 |
| title_sort | preliminary study of resistance mechanism of i botrytis cinerea i to syaup cn 26 |
| topic | SYAUP-CN-26 <i>Botrytis cinerea</i> activity cell membrane |
| url | https://www.mdpi.com/1420-3049/27/3/936 |
| work_keys_str_mv | AT kaiwang preliminarystudyofresistancemechanismofibotrytiscinereaitosyaupcn26 AT huazhongzhang preliminarystudyofresistancemechanismofibotrytiscinereaitosyaupcn26 AT wanyingzhu preliminarystudyofresistancemechanismofibotrytiscinereaitosyaupcn26 AT jingnanpeng preliminarystudyofresistancemechanismofibotrytiscinereaitosyaupcn26 AT xinghaili preliminarystudyofresistancemechanismofibotrytiscinereaitosyaupcn26 AT yingziwang preliminarystudyofresistancemechanismofibotrytiscinereaitosyaupcn26 AT zhiqiuqi preliminarystudyofresistancemechanismofibotrytiscinereaitosyaupcn26 |