Genomic Insights into the Microbial Agent <i>Streptomyces albidoflavus</i> MGMM6 for Various Biotechnology Applications
Microbial biotechnology plays a crucial role in improving industrial processes, particularly in the production of compounds with diverse applications. In this study, we used bioinformatic approaches to analyze the genomic architecture of <i>Streptomyces albidoflavus</i> MGMM6 and identif...
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| Format: | Article |
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MDPI AG
2023-11-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/11/12/2872 |
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| author | Roderic Gilles Claret Diabankana Mikhail Frolov Saparmyradov Keremli Shamil Zavdatovich Validov Daniel Mawuena Afordoanyi |
| author_facet | Roderic Gilles Claret Diabankana Mikhail Frolov Saparmyradov Keremli Shamil Zavdatovich Validov Daniel Mawuena Afordoanyi |
| author_sort | Roderic Gilles Claret Diabankana |
| collection | DOAJ |
| description | Microbial biotechnology plays a crucial role in improving industrial processes, particularly in the production of compounds with diverse applications. In this study, we used bioinformatic approaches to analyze the genomic architecture of <i>Streptomyces albidoflavus</i> MGMM6 and identify genes involved in various metabolic pathways that have significant biotechnological potential. Genome mining revealed that MGMM6 consists of a linear chromosome of 6,932,303 bp, with a high G+C content of 73.5%, lacking any plasmid contigs. Among the annotated genes, several are predicted to encode enzymes such as dye peroxidase, aromatic ring-opening dioxygenase, multicopper oxidase, cytochrome P450 monooxygenase, and aromatic ring hydroxylating dioxygenases which are responsible for the biodegradation of numerous endogenous and xenobiotic pollutants. In addition, we identified genes associated with heavy metal resistance, such as arsenic, cadmium, mercury, chromium, tellurium, antimony, and bismuth, suggesting the potential of MGMM6 for environmental remediation purposes. The analysis of secondary metabolites revealed the presence of multiple biosynthesis gene clusters responsible for producing compounds with potent antimicrobial and metal-chelating activities. Furthermore, laboratory tests conducted under controlled conditions demonstrated the effectiveness of MGMM6 in inhibiting phytopathogenic microbes, decolorizing and degrading aromatic triphenylmethane dyes, particularly Blue Brilliant G250, from wastewater by up to 98 ± 0.15%. Overall, the results of our study highlight the promising biotechnological potential of <i>S. albidoflavus</i> MGMM6. |
| first_indexed | 2024-03-08T20:30:41Z |
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| id | doaj.art-276ff8ca680748b6aa8c0222fee9281b |
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| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-08T20:30:41Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-276ff8ca680748b6aa8c0222fee9281b2023-12-22T14:25:50ZengMDPI AGMicroorganisms2076-26072023-11-011112287210.3390/microorganisms11122872Genomic Insights into the Microbial Agent <i>Streptomyces albidoflavus</i> MGMM6 for Various Biotechnology ApplicationsRoderic Gilles Claret Diabankana0Mikhail Frolov1Saparmyradov Keremli2Shamil Zavdatovich Validov3Daniel Mawuena Afordoanyi4Laboratory of Molecular Genetics and Microbiology Methods, Kazan Scientific Center of the Russian Academy of Sciences, 420111 Kazan, RussiaLaboratory of Molecular Genetics and Microbiology Methods, Kazan Scientific Center of the Russian Academy of Sciences, 420111 Kazan, RussiaLaboratory of Molecular Genetics and Microbiology Methods, Kazan Scientific Center of the Russian Academy of Sciences, 420111 Kazan, RussiaLaboratory of Molecular Genetics and Microbiology Methods, Kazan Scientific Center of the Russian Academy of Sciences, 420111 Kazan, RussiaLaboratory of Molecular Genetics and Microbiology Methods, Kazan Scientific Center of the Russian Academy of Sciences, 420111 Kazan, RussiaMicrobial biotechnology plays a crucial role in improving industrial processes, particularly in the production of compounds with diverse applications. In this study, we used bioinformatic approaches to analyze the genomic architecture of <i>Streptomyces albidoflavus</i> MGMM6 and identify genes involved in various metabolic pathways that have significant biotechnological potential. Genome mining revealed that MGMM6 consists of a linear chromosome of 6,932,303 bp, with a high G+C content of 73.5%, lacking any plasmid contigs. Among the annotated genes, several are predicted to encode enzymes such as dye peroxidase, aromatic ring-opening dioxygenase, multicopper oxidase, cytochrome P450 monooxygenase, and aromatic ring hydroxylating dioxygenases which are responsible for the biodegradation of numerous endogenous and xenobiotic pollutants. In addition, we identified genes associated with heavy metal resistance, such as arsenic, cadmium, mercury, chromium, tellurium, antimony, and bismuth, suggesting the potential of MGMM6 for environmental remediation purposes. The analysis of secondary metabolites revealed the presence of multiple biosynthesis gene clusters responsible for producing compounds with potent antimicrobial and metal-chelating activities. Furthermore, laboratory tests conducted under controlled conditions demonstrated the effectiveness of MGMM6 in inhibiting phytopathogenic microbes, decolorizing and degrading aromatic triphenylmethane dyes, particularly Blue Brilliant G250, from wastewater by up to 98 ± 0.15%. Overall, the results of our study highlight the promising biotechnological potential of <i>S. albidoflavus</i> MGMM6.https://www.mdpi.com/2076-2607/11/12/2872antimicrobial activitybioremediationbiodegradationbiocontrolbiotechnological applications<i>Fusarium oxysporum</i> |
| spellingShingle | Roderic Gilles Claret Diabankana Mikhail Frolov Saparmyradov Keremli Shamil Zavdatovich Validov Daniel Mawuena Afordoanyi Genomic Insights into the Microbial Agent <i>Streptomyces albidoflavus</i> MGMM6 for Various Biotechnology Applications Microorganisms antimicrobial activity bioremediation biodegradation biocontrol biotechnological applications <i>Fusarium oxysporum</i> |
| title | Genomic Insights into the Microbial Agent <i>Streptomyces albidoflavus</i> MGMM6 for Various Biotechnology Applications |
| title_full | Genomic Insights into the Microbial Agent <i>Streptomyces albidoflavus</i> MGMM6 for Various Biotechnology Applications |
| title_fullStr | Genomic Insights into the Microbial Agent <i>Streptomyces albidoflavus</i> MGMM6 for Various Biotechnology Applications |
| title_full_unstemmed | Genomic Insights into the Microbial Agent <i>Streptomyces albidoflavus</i> MGMM6 for Various Biotechnology Applications |
| title_short | Genomic Insights into the Microbial Agent <i>Streptomyces albidoflavus</i> MGMM6 for Various Biotechnology Applications |
| title_sort | genomic insights into the microbial agent i streptomyces albidoflavus i mgmm6 for various biotechnology applications |
| topic | antimicrobial activity bioremediation biodegradation biocontrol biotechnological applications <i>Fusarium oxysporum</i> |
| url | https://www.mdpi.com/2076-2607/11/12/2872 |
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