The Role of Nicotinic Receptors on Ca<sup>2+</sup> Signaling in Bovine Chromaffin Cells
Chromaffin cells have been used as a physiological model to understand neurosecretion in mammals for many years. Nicotinic receptors located in the cells’ membrane are stimulated by acetylcholine, and they participate in the exocytosis of chromaffin granules, releasing catecholamines in response to...
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MDPI AG
2024-01-01
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| Series: | Current Issues in Molecular Biology |
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| Online Access: | https://www.mdpi.com/1467-3045/46/1/52 |
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| author | Amparo Gil Virginia González-Vélez Luis Miguel Gutiérrez José Villanueva |
| author_facet | Amparo Gil Virginia González-Vélez Luis Miguel Gutiérrez José Villanueva |
| author_sort | Amparo Gil |
| collection | DOAJ |
| description | Chromaffin cells have been used as a physiological model to understand neurosecretion in mammals for many years. Nicotinic receptors located in the cells’ membrane are stimulated by acetylcholine, and they participate in the exocytosis of chromaffin granules, releasing catecholamines in response to stress. In this work, we discuss how the participation of nicotinic receptors and the localization of active zones in the borders of the cytoskeleton can generate local calcium signals leading to secretion. We use a computational model of a cytoskeleton cage to simulate Ca<sup>2+</sup> levels in response to voltage and acetylcholine pulses. We find that nicotinic receptors are able to enhance the differences between local and average calcium values, as well as the heterogeneous distributions around the active zones, producing a non-linear, highly localized Ca<sup>2+</sup> entry that, although consisting of a few ions, is able to improve secretion responses in chromaffin cells. Our findings emphasize the intricate interplay among nicotinic receptors, the cytoskeleton, and active zones within chromaffin cells as an example of Ca<sup>2+</sup>-dependent neurosecretion in mammals. |
| first_indexed | 2024-03-08T11:00:51Z |
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| issn | 1467-3037 1467-3045 |
| language | English |
| last_indexed | 2024-03-08T11:00:51Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
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| series | Current Issues in Molecular Biology |
| spelling | doaj.art-2770b703b88c42f797a54f337be75d432024-01-26T15:50:59ZengMDPI AGCurrent Issues in Molecular Biology1467-30371467-30452024-01-0146180882010.3390/cimb46010052The Role of Nicotinic Receptors on Ca<sup>2+</sup> Signaling in Bovine Chromaffin CellsAmparo Gil0Virginia González-Vélez1Luis Miguel Gutiérrez2José Villanueva3Departamento de Matemática Aplicada y CC de la Computación, Universidad de Cantabria, 39005 Santander, SpainDepartamento Ciencias Básicas, Universidad Autónoma Metropolitana Azcapotzalco, Mexico City 02128, MexicoInstituto de Neurociencias, CSIC-Universidad Miguel Hernández. Ctra de Valencia S/N, Sant Joan d’Alacant, 03550 Alicante, SpainInstituto de Neurociencias, CSIC-Universidad Miguel Hernández. Ctra de Valencia S/N, Sant Joan d’Alacant, 03550 Alicante, SpainChromaffin cells have been used as a physiological model to understand neurosecretion in mammals for many years. Nicotinic receptors located in the cells’ membrane are stimulated by acetylcholine, and they participate in the exocytosis of chromaffin granules, releasing catecholamines in response to stress. In this work, we discuss how the participation of nicotinic receptors and the localization of active zones in the borders of the cytoskeleton can generate local calcium signals leading to secretion. We use a computational model of a cytoskeleton cage to simulate Ca<sup>2+</sup> levels in response to voltage and acetylcholine pulses. We find that nicotinic receptors are able to enhance the differences between local and average calcium values, as well as the heterogeneous distributions around the active zones, producing a non-linear, highly localized Ca<sup>2+</sup> entry that, although consisting of a few ions, is able to improve secretion responses in chromaffin cells. Our findings emphasize the intricate interplay among nicotinic receptors, the cytoskeleton, and active zones within chromaffin cells as an example of Ca<sup>2+</sup>-dependent neurosecretion in mammals.https://www.mdpi.com/1467-3045/46/1/52Ca<sup>2+</sup>acetylcholine nicotinic receptorschromaffin cellsexocytosis |
| spellingShingle | Amparo Gil Virginia González-Vélez Luis Miguel Gutiérrez José Villanueva The Role of Nicotinic Receptors on Ca<sup>2+</sup> Signaling in Bovine Chromaffin Cells Current Issues in Molecular Biology Ca<sup>2+</sup> acetylcholine nicotinic receptors chromaffin cells exocytosis |
| title | The Role of Nicotinic Receptors on Ca<sup>2+</sup> Signaling in Bovine Chromaffin Cells |
| title_full | The Role of Nicotinic Receptors on Ca<sup>2+</sup> Signaling in Bovine Chromaffin Cells |
| title_fullStr | The Role of Nicotinic Receptors on Ca<sup>2+</sup> Signaling in Bovine Chromaffin Cells |
| title_full_unstemmed | The Role of Nicotinic Receptors on Ca<sup>2+</sup> Signaling in Bovine Chromaffin Cells |
| title_short | The Role of Nicotinic Receptors on Ca<sup>2+</sup> Signaling in Bovine Chromaffin Cells |
| title_sort | role of nicotinic receptors on ca sup 2 sup signaling in bovine chromaffin cells |
| topic | Ca<sup>2+</sup> acetylcholine nicotinic receptors chromaffin cells exocytosis |
| url | https://www.mdpi.com/1467-3045/46/1/52 |
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