Enhancement of individual differences in proliferation and differentiation potentials of aged human adipose-derived stem cells
Background: Adipose-derived stem cells (ASCs) are a robust, multipotent cell source. They are easily obtained and hold promise in many regenerative applications. It is generally considered that the function of somatic stem cells declines with age. Although several studies have examined the effects o...
| Main Authors: | , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2017-06-01
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| Series: | Regenerative Therapy |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352320417300032 |
| _version_ | 1819058433660289024 |
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| author | Mika Kawagishi-Hotta Seiji Hasegawa Toshio Igarashi Takaaki Yamada Masayuki Takahashi Shigeki Numata Tsukane Kobayashi Yohei Iwata Masaru Arima Naoki Yamamoto Akiko Yagami Satoru Nakata Tohru Uzawa Kayoko Matsunaga Kazumitsu Sugiura Hirohiko Akamatsu |
| author_facet | Mika Kawagishi-Hotta Seiji Hasegawa Toshio Igarashi Takaaki Yamada Masayuki Takahashi Shigeki Numata Tsukane Kobayashi Yohei Iwata Masaru Arima Naoki Yamamoto Akiko Yagami Satoru Nakata Tohru Uzawa Kayoko Matsunaga Kazumitsu Sugiura Hirohiko Akamatsu |
| author_sort | Mika Kawagishi-Hotta |
| collection | DOAJ |
| description | Background: Adipose-derived stem cells (ASCs) are a robust, multipotent cell source. They are easily obtained and hold promise in many regenerative applications. It is generally considered that the function of somatic stem cells declines with age. Although several studies have examined the effects of donor age on proliferation potential and pluripotency of ASCs, the results of these studies were not consistent.
Objective: This study tested whether the donor age affects the yield of ASCs from adipose tissue, as well as the proliferation and differentiation potentials of ASCs.
Methods: This study used ASCs obtained from adipose tissues of 260 donors (ages 5–97 years). ASCs were examined for individual differences in proliferation, and adipogenic, osteogenic and chondrogenic differentiation potentials in vitro. Characteristics of ASCs from each donor were evaluated by the principal component analysis (PCA) using their potential parameters.
Results: Analyses on ASCs demonstrated that adipogenic potentials declined with age, but proliferation, osteogenic and chondrogenic potentials were not correlated with age. Interestingly, in all ASC potentials, including adipogenesis, individual differences were observed. Principal component analysis (PCA) revealed that individual differences became evident in the elderly, and those variations were more prominent in females than in males.
Conclusions: This study demonstrated age-related changes in the potentials of ASCs and revealed that the individual differences of ASCs become significant in people over 60 years of age (for females over 60, and for males over 80). We believe that it is important to carefully observe ASC potentials in order to achieve effective regenerative medicine treatments using ASCs. |
| first_indexed | 2024-12-21T13:55:08Z |
| format | Article |
| id | doaj.art-277109bd496e422cb4ae867b855937c9 |
| institution | Directory Open Access Journal |
| issn | 2352-3204 |
| language | English |
| last_indexed | 2024-12-21T13:55:08Z |
| publishDate | 2017-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Regenerative Therapy |
| spelling | doaj.art-277109bd496e422cb4ae867b855937c92022-12-21T19:01:35ZengElsevierRegenerative Therapy2352-32042017-06-016C294010.1016/j.reth.2016.12.004Enhancement of individual differences in proliferation and differentiation potentials of aged human adipose-derived stem cellsMika Kawagishi-Hotta0Seiji Hasegawa1Toshio Igarashi2Takaaki Yamada3Masayuki Takahashi4Shigeki Numata5Tsukane Kobayashi6Yohei Iwata7Masaru Arima8Naoki Yamamoto9Akiko Yagami10Satoru Nakata11Tohru Uzawa12Kayoko Matsunaga13Kazumitsu Sugiura14Hirohiko Akamatsu15Research Laboratories, Nippon Menard Cosmetic Co., Ltd., 2-7 Torimi-cho, Nishi-ku, Nagoya, Aichi 451-0071, JapanResearch Laboratories, Nippon Menard Cosmetic Co., Ltd., 2-7 Torimi-cho, Nishi-ku, Nagoya, Aichi 451-0071, JapanResearch Laboratories, Nippon Menard Cosmetic Co., Ltd., 2-7 Torimi-cho, Nishi-ku, Nagoya, Aichi 451-0071, JapanResearch Laboratories, Nippon Menard Cosmetic Co., Ltd., 2-7 Torimi-cho, Nishi-ku, Nagoya, Aichi 451-0071, JapanDepartment of Dermatology, Fujita Health University School of Medicine, 1-98 Kutsukake-cho, Toyoake, Aichi 470-1192, JapanDepartment of Dermatology, Fujita Health University School of Medicine, 1-98 Kutsukake-cho, Toyoake, Aichi 470-1192, JapanDepartment of Dermatology, Fujita Health University School of Medicine, 1-98 Kutsukake-cho, Toyoake, Aichi 470-1192, JapanDepartment of Dermatology, Fujita Health University School of Medicine, 1-98 Kutsukake-cho, Toyoake, Aichi 470-1192, JapanDepartment of Dermatology, Fujita Health University School of Medicine, 1-98 Kutsukake-cho, Toyoake, Aichi 470-1192, JapanLaboratory of Molecular Biology & Histochemistry, Fujita Health University School of Medicine, 1-98 Kutsukake-cho, Toyoake, Aichi 470-1192, JapanDepartment of Dermatology, Fujita Health University School of Medicine, 1-98 Kutsukake-cho, Toyoake, Aichi 470-1192, JapanResearch Laboratories, Nippon Menard Cosmetic Co., Ltd., 2-7 Torimi-cho, Nishi-ku, Nagoya, Aichi 451-0071, JapanNagoya University Graduate School of Mathematics, Furo-cho, Chikusa-ku, Nagoya, Aichi 464-8602, JapanDepartment of Dermatology, Fujita Health University School of Medicine, 1-98 Kutsukake-cho, Toyoake, Aichi 470-1192, JapanDepartment of Dermatology, Fujita Health University School of Medicine, 1-98 Kutsukake-cho, Toyoake, Aichi 470-1192, JapanDepartment of Applied Cell and Regenerative Medicine, Fujita Health University School of Medicine, 1-98 Kutsukake-cho, Toyoake, Aichi 470-1192, JapanBackground: Adipose-derived stem cells (ASCs) are a robust, multipotent cell source. They are easily obtained and hold promise in many regenerative applications. It is generally considered that the function of somatic stem cells declines with age. Although several studies have examined the effects of donor age on proliferation potential and pluripotency of ASCs, the results of these studies were not consistent. Objective: This study tested whether the donor age affects the yield of ASCs from adipose tissue, as well as the proliferation and differentiation potentials of ASCs. Methods: This study used ASCs obtained from adipose tissues of 260 donors (ages 5–97 years). ASCs were examined for individual differences in proliferation, and adipogenic, osteogenic and chondrogenic differentiation potentials in vitro. Characteristics of ASCs from each donor were evaluated by the principal component analysis (PCA) using their potential parameters. Results: Analyses on ASCs demonstrated that adipogenic potentials declined with age, but proliferation, osteogenic and chondrogenic potentials were not correlated with age. Interestingly, in all ASC potentials, including adipogenesis, individual differences were observed. Principal component analysis (PCA) revealed that individual differences became evident in the elderly, and those variations were more prominent in females than in males. Conclusions: This study demonstrated age-related changes in the potentials of ASCs and revealed that the individual differences of ASCs become significant in people over 60 years of age (for females over 60, and for males over 80). We believe that it is important to carefully observe ASC potentials in order to achieve effective regenerative medicine treatments using ASCs.http://www.sciencedirect.com/science/article/pii/S2352320417300032Adipose-derived stem cellsDifferentiationIndividual differencesAging effectsPrincipal component analysis |
| spellingShingle | Mika Kawagishi-Hotta Seiji Hasegawa Toshio Igarashi Takaaki Yamada Masayuki Takahashi Shigeki Numata Tsukane Kobayashi Yohei Iwata Masaru Arima Naoki Yamamoto Akiko Yagami Satoru Nakata Tohru Uzawa Kayoko Matsunaga Kazumitsu Sugiura Hirohiko Akamatsu Enhancement of individual differences in proliferation and differentiation potentials of aged human adipose-derived stem cells Regenerative Therapy Adipose-derived stem cells Differentiation Individual differences Aging effects Principal component analysis |
| title | Enhancement of individual differences in proliferation and differentiation potentials of aged human adipose-derived stem cells |
| title_full | Enhancement of individual differences in proliferation and differentiation potentials of aged human adipose-derived stem cells |
| title_fullStr | Enhancement of individual differences in proliferation and differentiation potentials of aged human adipose-derived stem cells |
| title_full_unstemmed | Enhancement of individual differences in proliferation and differentiation potentials of aged human adipose-derived stem cells |
| title_short | Enhancement of individual differences in proliferation and differentiation potentials of aged human adipose-derived stem cells |
| title_sort | enhancement of individual differences in proliferation and differentiation potentials of aged human adipose derived stem cells |
| topic | Adipose-derived stem cells Differentiation Individual differences Aging effects Principal component analysis |
| url | http://www.sciencedirect.com/science/article/pii/S2352320417300032 |
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