Detection of post-harvest pathogens by loop-mediated isothermal amplification: a review
Postharvest losses, which occur between harvest and consumption of agricultural commodities, are major causes of food waste. Minimizing food loss helps provide nutritious food for animals and humans, and alleviate adverse environmental effects on food production. These losses are often related to th...
| मुख्य लेखकों: | , , , , , |
|---|---|
| स्वरूप: | लेख |
| भाषा: | English |
| प्रकाशित: |
Firenze University Press
2023-01-01
|
| श्रृंखला: | Phytopathologia Mediterranea |
| विषय: | |
| ऑनलाइन पहुंच: | https://oajournals.fupress.net/index.php/pm/article/view/14093 |
| _version_ | 1828064243158614016 |
|---|---|
| author | Wanissa MELLIKECHE Giulia CASINI Alessandra RICELLI Giancarlo COLELLI Marilita GALLO Anna Maria D'ONGHIA |
| author_facet | Wanissa MELLIKECHE Giulia CASINI Alessandra RICELLI Giancarlo COLELLI Marilita GALLO Anna Maria D'ONGHIA |
| author_sort | Wanissa MELLIKECHE |
| collection | DOAJ |
| description | Postharvest losses, which occur between harvest and consumption of agricultural commodities, are major causes of food waste. Minimizing food loss helps provide nutritious food for animals and humans, and alleviate adverse environmental effects on food production. These losses are often related to the presence of postharvest pathogens, including fungi and bacteria, which typically start by infecting crops in the field as well as during postharvest chain. Control of these pathogens relies on development of tools that ensure their early and accurate detection. Among these is loop-mediated isothermal amplification (LAMP), a molecular method for pathogen detection. LAMP characteristics of rapidity, specificity and simplicity have encouraged development of a number of LAMP assays for detection of postharvest pathogens. Each LAMP assay allows to detect a specific genetic region of the target microorganism, which can be directly related to mycotoxin production, fungicide resistance and phytotoxicity. The LAMP amplicons are rapidly visualized, either at a specific timepoint, or in real-time by taking measurements throughout reaction, thereby necessitating less sophisticated facilities than those needed for PCR assays. In addition, many studies have developed simple protocols for the direct detection of pathogens on fresh produce. This paper explains the LAMP reaction, and its importance for postharvest detection of fungi and bacteria. Previous studies that have developed LAMP assays are also discussed.
|
| first_indexed | 2024-04-10T22:56:34Z |
| format | Article |
| id | doaj.art-2772e719645d4e359bcb3c77d6c20ebc |
| institution | Directory Open Access Journal |
| issn | 0031-9465 1593-2095 |
| language | English |
| last_indexed | 2024-04-10T22:56:34Z |
| publishDate | 2023-01-01 |
| publisher | Firenze University Press |
| record_format | Article |
| series | Phytopathologia Mediterranea |
| spelling | doaj.art-2772e719645d4e359bcb3c77d6c20ebc2023-01-14T10:00:42ZengFirenze University PressPhytopathologia Mediterranea0031-94651593-20952023-01-0161310.36253/phyto-14093Detection of post-harvest pathogens by loop-mediated isothermal amplification: a reviewWanissa MELLIKECHE0Giulia CASINI1Alessandra RICELLI2Giancarlo COLELLI3Marilita GALLO4Anna Maria D'ONGHIA5Dipartimento di Scienze Agrarie, degli Alimenti e dell’Ambiente, Università di Foggia, Via Napoli 25, 71122 FoggiaEnbiotech S.r.l., Via Quarto dei Mille, 6, 90129 PalermoIstituto di Biologia e Patologia Molecolari (IBPM-CNR) P.le A. Moro 5, 00185, RomaDipartimento di Scienze Agrarie, degli Alimenti e dell’Ambiente, Università di Foggia, Via Napoli 25, 71122 FoggiaCentre International de Hautes Etudes Agronomiques Méditerranéennes Bari (CIHEAM Bari), Via Ceglie 9, 70010 Valenzano, BariCentre International de Hautes Etudes Agronomiques Méditerranéennes Bari (CIHEAM Bari), Via Ceglie 9, 70010 Valenzano, BariPostharvest losses, which occur between harvest and consumption of agricultural commodities, are major causes of food waste. Minimizing food loss helps provide nutritious food for animals and humans, and alleviate adverse environmental effects on food production. These losses are often related to the presence of postharvest pathogens, including fungi and bacteria, which typically start by infecting crops in the field as well as during postharvest chain. Control of these pathogens relies on development of tools that ensure their early and accurate detection. Among these is loop-mediated isothermal amplification (LAMP), a molecular method for pathogen detection. LAMP characteristics of rapidity, specificity and simplicity have encouraged development of a number of LAMP assays for detection of postharvest pathogens. Each LAMP assay allows to detect a specific genetic region of the target microorganism, which can be directly related to mycotoxin production, fungicide resistance and phytotoxicity. The LAMP amplicons are rapidly visualized, either at a specific timepoint, or in real-time by taking measurements throughout reaction, thereby necessitating less sophisticated facilities than those needed for PCR assays. In addition, many studies have developed simple protocols for the direct detection of pathogens on fresh produce. This paper explains the LAMP reaction, and its importance for postharvest detection of fungi and bacteria. Previous studies that have developed LAMP assays are also discussed. https://oajournals.fupress.net/index.php/pm/article/view/14093Food lossesmicroorganism contaminationmycotoxinsLAMP |
| spellingShingle | Wanissa MELLIKECHE Giulia CASINI Alessandra RICELLI Giancarlo COLELLI Marilita GALLO Anna Maria D'ONGHIA Detection of post-harvest pathogens by loop-mediated isothermal amplification: a review Phytopathologia Mediterranea Food losses microorganism contamination mycotoxins LAMP |
| title | Detection of post-harvest pathogens by loop-mediated isothermal amplification: a review |
| title_full | Detection of post-harvest pathogens by loop-mediated isothermal amplification: a review |
| title_fullStr | Detection of post-harvest pathogens by loop-mediated isothermal amplification: a review |
| title_full_unstemmed | Detection of post-harvest pathogens by loop-mediated isothermal amplification: a review |
| title_short | Detection of post-harvest pathogens by loop-mediated isothermal amplification: a review |
| title_sort | detection of post harvest pathogens by loop mediated isothermal amplification a review |
| topic | Food losses microorganism contamination mycotoxins LAMP |
| url | https://oajournals.fupress.net/index.php/pm/article/view/14093 |
| work_keys_str_mv | AT wanissamellikeche detectionofpostharvestpathogensbyloopmediatedisothermalamplificationareview AT giuliacasini detectionofpostharvestpathogensbyloopmediatedisothermalamplificationareview AT alessandraricelli detectionofpostharvestpathogensbyloopmediatedisothermalamplificationareview AT giancarlocolelli detectionofpostharvestpathogensbyloopmediatedisothermalamplificationareview AT marilitagallo detectionofpostharvestpathogensbyloopmediatedisothermalamplificationareview AT annamariadonghia detectionofpostharvestpathogensbyloopmediatedisothermalamplificationareview |