Characterization and genome analysis of a butanol–isopropanol-producing Clostridium beijerinckii strain BGS1
Abstract Background One of the main challenges of acetone–butanol–ethanol fermentation is to reduce acetone production with high butanol yield. Converting acetone into isopropanol is an alternative pathway to reduce fermentation by-products in the fermentation broth. Here, we aimed to cultivate a wi...
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| Language: | English |
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BMC
2018-10-01
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| Series: | Biotechnology for Biofuels |
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| Online Access: | http://link.springer.com/article/10.1186/s13068-018-1274-x |
| _version_ | 1817977082713473024 |
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| author | Chen Zhang Tinggang Li Jianzhong He |
| author_facet | Chen Zhang Tinggang Li Jianzhong He |
| author_sort | Chen Zhang |
| collection | DOAJ |
| description | Abstract Background One of the main challenges of acetone–butanol–ethanol fermentation is to reduce acetone production with high butanol yield. Converting acetone into isopropanol is an alternative pathway to reduce fermentation by-products in the fermentation broth. Here, we aimed to cultivate a wild-type Clostridium strain with high isopropanol and butanol production and reveal its genome information. Results Clostridium beijerinckii strain BGS1 was found to be capable of producing 10.21 g/L butanol and 3.41 g/L isopropanol, higher than previously known wild-type isopropanol–butanol-producing Clostridium species. Moreover, culture BGS1 exhibited a broad carbon spectrum utilizing diverse sugars such as arabinose, xylose, galactose, cellobiose, and sucrose, with 9.61 g/L butanol and 2.57 g/L isopropanol generated from 60 g/L sucrose and less amount from other sugars. Based on genome analysis, protein-based sequence of strain BGS1 was closer to C. beijerinckii NCIMB 8052, reaching 90.82% similarity, while compared to C. beijerinckii DSM 6423, the similarity was 89.53%. In addition, a unique secondary alcohol dehydrogenase (sAdhE) was revealed in the genome of strain BGS1, which distinguished it from other Clostridium species. Average nucleotide identity analysis identified strain BGS1 belonging to C. beijerinckii. The transcription profile and enzymatic activity of sAdhE proved its function of converting acetone into isopropanol. Conclusions Clostridium beijerinckii strain BGS1 is a potential candidate for industrial isopropanol and butanol production. Its genome provides unique information for genetic engineering of isopropanol–butanol-producing microorganisms. |
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| format | Article |
| id | doaj.art-2773cc553b52485f9a2e1669b5df7384 |
| institution | Directory Open Access Journal |
| issn | 1754-6834 |
| language | English |
| last_indexed | 2024-04-13T22:12:32Z |
| publishDate | 2018-10-01 |
| publisher | BMC |
| record_format | Article |
| series | Biotechnology for Biofuels |
| spelling | doaj.art-2773cc553b52485f9a2e1669b5df73842022-12-22T02:27:41ZengBMCBiotechnology for Biofuels1754-68342018-10-0111111110.1186/s13068-018-1274-xCharacterization and genome analysis of a butanol–isopropanol-producing Clostridium beijerinckii strain BGS1Chen Zhang0Tinggang Li1Jianzhong He2Department of Civil and Environmental Engineering, National University of SingaporeDepartment of Civil and Environmental Engineering, National University of SingaporeDepartment of Civil and Environmental Engineering, National University of SingaporeAbstract Background One of the main challenges of acetone–butanol–ethanol fermentation is to reduce acetone production with high butanol yield. Converting acetone into isopropanol is an alternative pathway to reduce fermentation by-products in the fermentation broth. Here, we aimed to cultivate a wild-type Clostridium strain with high isopropanol and butanol production and reveal its genome information. Results Clostridium beijerinckii strain BGS1 was found to be capable of producing 10.21 g/L butanol and 3.41 g/L isopropanol, higher than previously known wild-type isopropanol–butanol-producing Clostridium species. Moreover, culture BGS1 exhibited a broad carbon spectrum utilizing diverse sugars such as arabinose, xylose, galactose, cellobiose, and sucrose, with 9.61 g/L butanol and 2.57 g/L isopropanol generated from 60 g/L sucrose and less amount from other sugars. Based on genome analysis, protein-based sequence of strain BGS1 was closer to C. beijerinckii NCIMB 8052, reaching 90.82% similarity, while compared to C. beijerinckii DSM 6423, the similarity was 89.53%. In addition, a unique secondary alcohol dehydrogenase (sAdhE) was revealed in the genome of strain BGS1, which distinguished it from other Clostridium species. Average nucleotide identity analysis identified strain BGS1 belonging to C. beijerinckii. The transcription profile and enzymatic activity of sAdhE proved its function of converting acetone into isopropanol. Conclusions Clostridium beijerinckii strain BGS1 is a potential candidate for industrial isopropanol and butanol production. Its genome provides unique information for genetic engineering of isopropanol–butanol-producing microorganisms.http://link.springer.com/article/10.1186/s13068-018-1274-xIsopropanolButanolClostridium beijerinckiiGenomeSecondary alcohol dehydrogenase |
| spellingShingle | Chen Zhang Tinggang Li Jianzhong He Characterization and genome analysis of a butanol–isopropanol-producing Clostridium beijerinckii strain BGS1 Biotechnology for Biofuels Isopropanol Butanol Clostridium beijerinckii Genome Secondary alcohol dehydrogenase |
| title | Characterization and genome analysis of a butanol–isopropanol-producing Clostridium beijerinckii strain BGS1 |
| title_full | Characterization and genome analysis of a butanol–isopropanol-producing Clostridium beijerinckii strain BGS1 |
| title_fullStr | Characterization and genome analysis of a butanol–isopropanol-producing Clostridium beijerinckii strain BGS1 |
| title_full_unstemmed | Characterization and genome analysis of a butanol–isopropanol-producing Clostridium beijerinckii strain BGS1 |
| title_short | Characterization and genome analysis of a butanol–isopropanol-producing Clostridium beijerinckii strain BGS1 |
| title_sort | characterization and genome analysis of a butanol isopropanol producing clostridium beijerinckii strain bgs1 |
| topic | Isopropanol Butanol Clostridium beijerinckii Genome Secondary alcohol dehydrogenase |
| url | http://link.springer.com/article/10.1186/s13068-018-1274-x |
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