Correlation and influencing factors analysis of the cycle threshold value in detection of novel coronavirus nucleic acid(Omicron) and the negative conversion cycle in infected patients
Objective: To explore the relationship between the cycle threshold (Ct) value of ORF1ab gene and N gene in the first admission detected by novel coronavirus (Omicron) nucleic acid and the negative conversion cycle of infected patients. Methods: 5 212 patients with novel coronavirus omicron variant i...
| Main Author: | |
|---|---|
| Format: | Article |
| Language: | zho |
| Published: |
Editorial Office of Journal of Diagnostics Concepts & Practice
2022-04-01
|
| Series: | Zhenduanxue lilun yu shijian |
| Subjects: | |
| Online Access: | http://www.qk.sjtu.edu.cn/jdcp/fileup/1671-2870/PDF/1654694219524-1457203836.pdf |
| _version_ | 1797975573186740224 |
|---|---|
| author | DING Ning, CHEN Shikai, MENG Jun, DAI Jing, JIN Peipei, et al |
| author_facet | DING Ning, CHEN Shikai, MENG Jun, DAI Jing, JIN Peipei, et al |
| author_sort | DING Ning, CHEN Shikai, MENG Jun, DAI Jing, JIN Peipei, et al |
| collection | DOAJ |
| description | Objective: To explore the relationship between the cycle threshold (Ct) value of ORF1ab gene and N gene in the first admission detected by novel coronavirus (Omicron) nucleic acid and the negative conversion cycle of infected patients. Methods: 5 212 patients with novel coronavirus omicron variant infection were admitted to the Northern Ruijin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine from March 2022 to April 2022 were collected, according to the patient′s previous history and the tests of relevant indexes after admission, including 2 746 cases in non-basic disease group and 2 466 cases in the basic disease group. All subjects received the Ct value of the ORF1ab genes and the N genes tests. Statistical analysis of the difference of the negative conversion cycle of ORF1ab gene and N gene between non-basic disease group and basic disease group under different Ct values at the first admission. Pearson linear correlation analysis was used to assess the association of Ct value of ORF1ab gene and N gene with the negative conversion cycle, and multiple linear regression was used to analyze the factors affecting the negative conversion cycle. Results: The Ct values of ORF1ab gene and N gene in patients novel coronavirus infection were negatively correlated with the negative conversion cycle (rORF1ab=-0.4622, P<0.01; rN=-0.5428, P<0.01). In the case of Ct value ≤20 and 20 <Ct value ≤30, there was a significant difference in the negative conversion cycle between the group with basic diseases and the group with non-basic disease, which the negative conversion cycle of the group with basic diseases was significantly higher than that of the group with non-basic disease (P<0.05); When the Ct value of the ORF1ab gene increases by one cycle, the negative conversion cycle is shortened by 0.21 days, and when the Ct value of the N gene increases by one cycle, the negative conversion cycle is shortened by 0.26 days, in addition, age and basic diseases are risk factors. Conclusions: The Ct values of ORF1ab gene and N gene in the first admission are related to the negative conversion cycle of patients with novel coronavirus (Omicron) infection, which can be used as an independent predictor of the negative conversion cycle of patients with novel coronavirus infection and have a certain value in guiding patients with novel coronavirus infection to reduce the frequency of nucleic acid detection and to assess disease progression. |
| first_indexed | 2024-04-11T04:37:42Z |
| format | Article |
| id | doaj.art-2773d531293d44c78b675107f27edefe |
| institution | Directory Open Access Journal |
| issn | 1671-2870 |
| language | zho |
| last_indexed | 2024-04-11T04:37:42Z |
| publishDate | 2022-04-01 |
| publisher | Editorial Office of Journal of Diagnostics Concepts & Practice |
| record_format | Article |
| series | Zhenduanxue lilun yu shijian |
| spelling | doaj.art-2773d531293d44c78b675107f27edefe2022-12-28T08:22:29ZzhoEditorial Office of Journal of Diagnostics Concepts & PracticeZhenduanxue lilun yu shijian1671-28702022-04-01210216917310.16150/j.1671-2870.2022.02.013Correlation and influencing factors analysis of the cycle threshold value in detection of novel coronavirus nucleic acid(Omicron) and the negative conversion cycle in infected patientsDING Ning, CHEN Shikai, MENG Jun, DAI Jing, JIN Peipei, et al0Department of Laboratory Medicine, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 201801, ChinaObjective: To explore the relationship between the cycle threshold (Ct) value of ORF1ab gene and N gene in the first admission detected by novel coronavirus (Omicron) nucleic acid and the negative conversion cycle of infected patients. Methods: 5 212 patients with novel coronavirus omicron variant infection were admitted to the Northern Ruijin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine from March 2022 to April 2022 were collected, according to the patient′s previous history and the tests of relevant indexes after admission, including 2 746 cases in non-basic disease group and 2 466 cases in the basic disease group. All subjects received the Ct value of the ORF1ab genes and the N genes tests. Statistical analysis of the difference of the negative conversion cycle of ORF1ab gene and N gene between non-basic disease group and basic disease group under different Ct values at the first admission. Pearson linear correlation analysis was used to assess the association of Ct value of ORF1ab gene and N gene with the negative conversion cycle, and multiple linear regression was used to analyze the factors affecting the negative conversion cycle. Results: The Ct values of ORF1ab gene and N gene in patients novel coronavirus infection were negatively correlated with the negative conversion cycle (rORF1ab=-0.4622, P<0.01; rN=-0.5428, P<0.01). In the case of Ct value ≤20 and 20 <Ct value ≤30, there was a significant difference in the negative conversion cycle between the group with basic diseases and the group with non-basic disease, which the negative conversion cycle of the group with basic diseases was significantly higher than that of the group with non-basic disease (P<0.05); When the Ct value of the ORF1ab gene increases by one cycle, the negative conversion cycle is shortened by 0.21 days, and when the Ct value of the N gene increases by one cycle, the negative conversion cycle is shortened by 0.26 days, in addition, age and basic diseases are risk factors. Conclusions: The Ct values of ORF1ab gene and N gene in the first admission are related to the negative conversion cycle of patients with novel coronavirus (Omicron) infection, which can be used as an independent predictor of the negative conversion cycle of patients with novel coronavirus infection and have a certain value in guiding patients with novel coronavirus infection to reduce the frequency of nucleic acid detection and to assess disease progression.http://www.qk.sjtu.edu.cn/jdcp/fileup/1671-2870/PDF/1654694219524-1457203836.pdf|novel coronavirus|orf1ab gene|n gene|cycle threshold value|negative conversion cycle |
| spellingShingle | DING Ning, CHEN Shikai, MENG Jun, DAI Jing, JIN Peipei, et al Correlation and influencing factors analysis of the cycle threshold value in detection of novel coronavirus nucleic acid(Omicron) and the negative conversion cycle in infected patients Zhenduanxue lilun yu shijian |novel coronavirus|orf1ab gene|n gene|cycle threshold value|negative conversion cycle |
| title | Correlation and influencing factors analysis of the cycle threshold value in detection of novel coronavirus nucleic acid(Omicron) and the negative conversion cycle in infected patients |
| title_full | Correlation and influencing factors analysis of the cycle threshold value in detection of novel coronavirus nucleic acid(Omicron) and the negative conversion cycle in infected patients |
| title_fullStr | Correlation and influencing factors analysis of the cycle threshold value in detection of novel coronavirus nucleic acid(Omicron) and the negative conversion cycle in infected patients |
| title_full_unstemmed | Correlation and influencing factors analysis of the cycle threshold value in detection of novel coronavirus nucleic acid(Omicron) and the negative conversion cycle in infected patients |
| title_short | Correlation and influencing factors analysis of the cycle threshold value in detection of novel coronavirus nucleic acid(Omicron) and the negative conversion cycle in infected patients |
| title_sort | correlation and influencing factors analysis of the cycle threshold value in detection of novel coronavirus nucleic acid omicron and the negative conversion cycle in infected patients |
| topic | |novel coronavirus|orf1ab gene|n gene|cycle threshold value|negative conversion cycle |
| url | http://www.qk.sjtu.edu.cn/jdcp/fileup/1671-2870/PDF/1654694219524-1457203836.pdf |
| work_keys_str_mv | AT dingningchenshikaimengjundaijingjinpeipeietal correlationandinfluencingfactorsanalysisofthecyclethresholdvalueindetectionofnovelcoronavirusnucleicacidomicronandthenegativeconversioncycleininfectedpatients |