| Summary: | Objective: To investigate the effect of all-trans retinoic acid (ATRA) on Cx43 protein expression and GJIC function in IL-22-treated HaCaT cells, and whether it is related to the JNK pathway to further elaborate molecular mechanism of ATRA on GJIC function. Methods: The optimal pretreated time affected by ATRA on HaCaT cells was screened in IL-22 treatment group.Scrape-loading dye-transfer assay was applied to observe the variation of GJIC function.The expression value of Cx43, p-JNK, JNK in ATRA and/or IL-22 treatment group were detected by immunofluorescence. Results: ATRA caused time-dependent increasing of GJIC function in HaCaT cells. After treatment of ATRA on IL-22 induced HaCaT cells, Cx43 protein expression level increased, GJIC function up-regulated, and p-JNK expression had not significant change. Conclusions: ATRA inhibited the decrease of Cx43 expression by IL-22-mediated and down-regulation of GJIC, and this process was not related to the JNK pathway.
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