Upregulation of the MYB2 Transcription Factor is Associated with Increased Accumulation of Anthocyanin in the Leaves of <i>Dendrobium bigibbum</i>
Orchids with colorful leaves and flowers have significant ornamental value. Here, we used γ-irradiation-based mutagenesis to produce a <i>Dendrobium bigibbum</i> mutant that developed purple instead of the normal green leaves. RNA sequencing of the mutant plant identified 2513 differenti...
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| Format: | Article |
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MDPI AG
2020-08-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/21/16/5653 |
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| author | Gah-Hyun Lim Se Won Kim Jaihyunk Ryu Si-Yong Kang Jin-Baek Kim Sang Hoon Kim |
| author_facet | Gah-Hyun Lim Se Won Kim Jaihyunk Ryu Si-Yong Kang Jin-Baek Kim Sang Hoon Kim |
| author_sort | Gah-Hyun Lim |
| collection | DOAJ |
| description | Orchids with colorful leaves and flowers have significant ornamental value. Here, we used γ-irradiation-based mutagenesis to produce a <i>Dendrobium bigibbum</i> mutant that developed purple instead of the normal green leaves. RNA sequencing of the mutant plant identified 2513 differentially expressed genes, including 1870 up- and 706 downregulated genes. The purple leaf color of mutant leaves was associated with increased expression of genes that encoded key biosynthetic enzymes in the anthocyanin biosynthetic pathway. In addition, the mutant leaves also showed increased expression of several families of transcription factors including the <i>MYB2</i> gene. Transient overexpression of <i>D. biggibum</i><i>MYB2</i> in <i>Nicotiana benthamiana</i> was associated with increased expression of endogenous anthocyanin biosynthesis genes. Interestingly, transient overexpression of orthologous <i>MYB2</i> genes from other orchids did not upregulate expression of endogenous anthocyanin biosynthesis genes. Together, these results suggest that the purple coloration of <i>D. biggibum</i> leaves is at least associated with increased expression of the <i>MYB2</i> gene, and the <i>MYB2</i> orthologs from orchids likely function differently, regardless of their high level of similarity. |
| first_indexed | 2024-03-10T17:51:36Z |
| format | Article |
| id | doaj.art-27774a13e6b5444fbe26544ad7a15b12 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T17:51:36Z |
| publishDate | 2020-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-27774a13e6b5444fbe26544ad7a15b122023-11-20T09:20:33ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-08-012116565310.3390/ijms21165653Upregulation of the MYB2 Transcription Factor is Associated with Increased Accumulation of Anthocyanin in the Leaves of <i>Dendrobium bigibbum</i>Gah-Hyun Lim0Se Won Kim1Jaihyunk Ryu2Si-Yong Kang3Jin-Baek Kim4Sang Hoon Kim5Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute (KAERI), Jeongeup 56212, KoreaAdvanced Radiation Technology Institute, Korea Atomic Energy Research Institute (KAERI), Jeongeup 56212, KoreaAdvanced Radiation Technology Institute, Korea Atomic Energy Research Institute (KAERI), Jeongeup 56212, KoreaAdvanced Radiation Technology Institute, Korea Atomic Energy Research Institute (KAERI), Jeongeup 56212, KoreaAdvanced Radiation Technology Institute, Korea Atomic Energy Research Institute (KAERI), Jeongeup 56212, KoreaAdvanced Radiation Technology Institute, Korea Atomic Energy Research Institute (KAERI), Jeongeup 56212, KoreaOrchids with colorful leaves and flowers have significant ornamental value. Here, we used γ-irradiation-based mutagenesis to produce a <i>Dendrobium bigibbum</i> mutant that developed purple instead of the normal green leaves. RNA sequencing of the mutant plant identified 2513 differentially expressed genes, including 1870 up- and 706 downregulated genes. The purple leaf color of mutant leaves was associated with increased expression of genes that encoded key biosynthetic enzymes in the anthocyanin biosynthetic pathway. In addition, the mutant leaves also showed increased expression of several families of transcription factors including the <i>MYB2</i> gene. Transient overexpression of <i>D. biggibum</i><i>MYB2</i> in <i>Nicotiana benthamiana</i> was associated with increased expression of endogenous anthocyanin biosynthesis genes. Interestingly, transient overexpression of orthologous <i>MYB2</i> genes from other orchids did not upregulate expression of endogenous anthocyanin biosynthesis genes. Together, these results suggest that the purple coloration of <i>D. biggibum</i> leaves is at least associated with increased expression of the <i>MYB2</i> gene, and the <i>MYB2</i> orthologs from orchids likely function differently, regardless of their high level of similarity.https://www.mdpi.com/1422-0067/21/16/5653anthocyaninMYB2orchid<i>Dendrobium bigibbum</i>γ-irradiation |
| spellingShingle | Gah-Hyun Lim Se Won Kim Jaihyunk Ryu Si-Yong Kang Jin-Baek Kim Sang Hoon Kim Upregulation of the MYB2 Transcription Factor is Associated with Increased Accumulation of Anthocyanin in the Leaves of <i>Dendrobium bigibbum</i> International Journal of Molecular Sciences anthocyanin MYB2 orchid <i>Dendrobium bigibbum</i> γ-irradiation |
| title | Upregulation of the MYB2 Transcription Factor is Associated with Increased Accumulation of Anthocyanin in the Leaves of <i>Dendrobium bigibbum</i> |
| title_full | Upregulation of the MYB2 Transcription Factor is Associated with Increased Accumulation of Anthocyanin in the Leaves of <i>Dendrobium bigibbum</i> |
| title_fullStr | Upregulation of the MYB2 Transcription Factor is Associated with Increased Accumulation of Anthocyanin in the Leaves of <i>Dendrobium bigibbum</i> |
| title_full_unstemmed | Upregulation of the MYB2 Transcription Factor is Associated with Increased Accumulation of Anthocyanin in the Leaves of <i>Dendrobium bigibbum</i> |
| title_short | Upregulation of the MYB2 Transcription Factor is Associated with Increased Accumulation of Anthocyanin in the Leaves of <i>Dendrobium bigibbum</i> |
| title_sort | upregulation of the myb2 transcription factor is associated with increased accumulation of anthocyanin in the leaves of i dendrobium bigibbum i |
| topic | anthocyanin MYB2 orchid <i>Dendrobium bigibbum</i> γ-irradiation |
| url | https://www.mdpi.com/1422-0067/21/16/5653 |
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