Derivation and culturing of neural stem cells from human embryonic brain tissue
Summary: Human neural stem cells (hNSCs) are a valuable tool in brain cancer research since they are used as a normal control for multiple assays, mainly pertaining to toxicity. Here, we present a protocol to safely and successfully derive and culture hNSCs in vitro from human embryonic brain tissue...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-09-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166722005081 |
| _version_ | 1811284160163086336 |
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| author | Yujin Suk Agata Kieliszek Daniel Mobilio Chitra Venugopal Sheila K. Singh |
| author_facet | Yujin Suk Agata Kieliszek Daniel Mobilio Chitra Venugopal Sheila K. Singh |
| author_sort | Yujin Suk |
| collection | DOAJ |
| description | Summary: Human neural stem cells (hNSCs) are a valuable tool in brain cancer research since they are used as a normal control for multiple assays, mainly pertaining to toxicity. Here, we present a protocol to safely and successfully derive and culture hNSCs in vitro from human embryonic brain tissue. We describe the steps to dissociate embryonic brain tissue and culture hNSCs, followed by the procedure to expand hNSCs. These cells can be used for downstream applications including RNA-seq and omics studies.For complete details on the use and execution of this protocol, please refer to Venugopal et al. (2012b), Bakhshinyan et al. (2019), and Venugopal et al. (2012a). : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| first_indexed | 2024-04-13T02:24:32Z |
| format | Article |
| id | doaj.art-2777d6ce58714b06b642178c4b97da57 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-04-13T02:24:32Z |
| publishDate | 2022-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-2777d6ce58714b06b642178c4b97da572022-12-22T03:06:49ZengElsevierSTAR Protocols2666-16672022-09-0133101628Derivation and culturing of neural stem cells from human embryonic brain tissueYujin Suk0Agata Kieliszek1Daniel Mobilio2Chitra Venugopal3Sheila K. Singh4Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, ON L8S 4L8, Canada; Michael G DeGroote School of Medicine, McMaster University, Hamilton, ON L8S 4L8, Canada; Corresponding authorDepartment of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, ON L8S 4L8, CanadaDepartment of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, ON L8S 4L8, CanadaDepartment of Surgery, McMaster University, Hamilton, ON L8S 4L8, CanadaDepartment of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, ON L8S 4L8, Canada; Department of Surgery, McMaster University, Hamilton, ON L8S 4L8, Canada; Corresponding authorSummary: Human neural stem cells (hNSCs) are a valuable tool in brain cancer research since they are used as a normal control for multiple assays, mainly pertaining to toxicity. Here, we present a protocol to safely and successfully derive and culture hNSCs in vitro from human embryonic brain tissue. We describe the steps to dissociate embryonic brain tissue and culture hNSCs, followed by the procedure to expand hNSCs. These cells can be used for downstream applications including RNA-seq and omics studies.For complete details on the use and execution of this protocol, please refer to Venugopal et al. (2012b), Bakhshinyan et al. (2019), and Venugopal et al. (2012a). : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166722005081Cell cultureCell isolationDevelopmental biologyHealth sciencesNeuroscienceStem cells |
| spellingShingle | Yujin Suk Agata Kieliszek Daniel Mobilio Chitra Venugopal Sheila K. Singh Derivation and culturing of neural stem cells from human embryonic brain tissue STAR Protocols Cell culture Cell isolation Developmental biology Health sciences Neuroscience Stem cells |
| title | Derivation and culturing of neural stem cells from human embryonic brain tissue |
| title_full | Derivation and culturing of neural stem cells from human embryonic brain tissue |
| title_fullStr | Derivation and culturing of neural stem cells from human embryonic brain tissue |
| title_full_unstemmed | Derivation and culturing of neural stem cells from human embryonic brain tissue |
| title_short | Derivation and culturing of neural stem cells from human embryonic brain tissue |
| title_sort | derivation and culturing of neural stem cells from human embryonic brain tissue |
| topic | Cell culture Cell isolation Developmental biology Health sciences Neuroscience Stem cells |
| url | http://www.sciencedirect.com/science/article/pii/S2666166722005081 |
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