The uppermost monoterpenes improving Cinnamomum camphora thermotolerance by serving signaling functions
Terpenes serve important functions in enhancing plant thermotolerance. Cinnamomum camphora mainly has eucalyptol (EuL), camphor (CmR), linalool (LnL) and borneol (BeL) chemotypes basing on the uppermost monoterpenes. To reveal the thermotolerance mechanisms of these uppermost monoterpenes (eucalypto...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-12-01
|
| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.1072931/full |
| _version_ | 1811177726546018304 |
|---|---|
| author | Chenyi Xu Chenyi Xu Bin Wang Bin Wang Qingyun Luo Qingyun Luo Yuandan Ma Yuandan Ma Tiefeng Zheng Tiefeng Zheng Yingying Wang Yingying Wang Yuyan Cai Yuyan Cai Zhaojiang Zuo Zhaojiang Zuo |
| author_facet | Chenyi Xu Chenyi Xu Bin Wang Bin Wang Qingyun Luo Qingyun Luo Yuandan Ma Yuandan Ma Tiefeng Zheng Tiefeng Zheng Yingying Wang Yingying Wang Yuyan Cai Yuyan Cai Zhaojiang Zuo Zhaojiang Zuo |
| author_sort | Chenyi Xu |
| collection | DOAJ |
| description | Terpenes serve important functions in enhancing plant thermotolerance. Cinnamomum camphora mainly has eucalyptol (EuL), camphor (CmR), linalool (LnL) and borneol (BeL) chemotypes basing on the uppermost monoterpenes. To reveal the thermotolerance mechanisms of these uppermost monoterpenes (eucalyptol, camphor, linalool, and borneol) in C. camphora, we surveyed the ROS metabolism and photosynthesis in the 4 chemotypes fumigated with the corresponding uppermost monoterpene after fosmidomycin (Fos) inhibiting monoterpene synthesis under high temperature at 38°C (Fos+38°C+monoterpene), and investigated the related gene expression in EuL and CmR. Meanwhile, the thermotolerance differences among the 4 uppermost monoterpenes were analyzed. In contrast to normal temperature (28°C), ROS levels and antioxidant enzyme activities in the 4 chemotypes increased under 38°C, and further increased in the treatment with Fos inhibiting monoterpene synthesis at 38°C (Fos+38°C), which may be caused by the alterations in expression of the genes related with non-enzymatic and enzymatic antioxidant formation according to the analyses in EuL and CmR. Compared with Fos+38°C treatment, Fos+38°C+monoterpene treatments lowered ROS levels and antioxidant enzyme activities for the increased non-enzymatic antioxidant gene expression and decreased enzymatic antioxidant gene expression, respectively. High temperature at 38°C reduced the chlorophyll and carotenoid content as well as photosynthetic abilities, which may result from the declined expression of the genes associated with photosynthetic pigment biosynthesis, light reaction, and carbon fixation. Fos+38°C treatment aggravated the reduction. In contrast to Fos+38°C treatment, Fos+38°C+monoterpene treatments increased photosynthetic pigment content and improved photosynthetic abilities by up-regulating related gene expression. Among the 4 uppermost monoterpenes, camphor showed strong abilities in lowering ROS and maintaining photosynthesis, while eucalyptol showed weak abilities. This was consistent with the recovery effects of the gene expression in the treatments with camphor and eucalyptol fumigation. Therefore, the uppermost monoterpenes can enhance C. camphora thermotolerance as signaling molecules, and may have differences in the signaling functions. |
| first_indexed | 2024-04-11T06:07:17Z |
| format | Article |
| id | doaj.art-27787a88a91b43228732ecba1f253db1 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-11T06:07:17Z |
| publishDate | 2022-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-27787a88a91b43228732ecba1f253db12022-12-22T04:41:28ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-12-011310.3389/fpls.2022.10729311072931The uppermost monoterpenes improving Cinnamomum camphora thermotolerance by serving signaling functionsChenyi Xu0Chenyi Xu1Bin Wang2Bin Wang3Qingyun Luo4Qingyun Luo5Yuandan Ma6Yuandan Ma7Tiefeng Zheng8Tiefeng Zheng9Yingying Wang10Yingying Wang11Yuyan Cai12Yuyan Cai13Zhaojiang Zuo14Zhaojiang Zuo15State Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Hangzhou, ChinaZhejiang Provincial Key Laboratory of Forest Aromatic Plants-based Healthcare Functions, Zhejiang A&F University, Hangzhou, ChinaState Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Hangzhou, ChinaZhejiang Provincial Key Laboratory of Forest Aromatic Plants-based Healthcare Functions, Zhejiang A&F University, Hangzhou, ChinaState Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Hangzhou, ChinaZhejiang Provincial Key Laboratory of Forest Aromatic Plants-based Healthcare Functions, Zhejiang A&F University, Hangzhou, ChinaState Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Hangzhou, ChinaZhejiang Provincial Key Laboratory of Forest Aromatic Plants-based Healthcare Functions, Zhejiang A&F University, Hangzhou, ChinaState Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Hangzhou, ChinaZhejiang Provincial Key Laboratory of Forest Aromatic Plants-based Healthcare Functions, Zhejiang A&F University, Hangzhou, ChinaState Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Hangzhou, ChinaZhejiang Provincial Key Laboratory of Forest Aromatic Plants-based Healthcare Functions, Zhejiang A&F University, Hangzhou, ChinaState Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Hangzhou, ChinaZhejiang Provincial Key Laboratory of Forest Aromatic Plants-based Healthcare Functions, Zhejiang A&F University, Hangzhou, ChinaState Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Hangzhou, ChinaZhejiang Provincial Key Laboratory of Forest Aromatic Plants-based Healthcare Functions, Zhejiang A&F University, Hangzhou, ChinaTerpenes serve important functions in enhancing plant thermotolerance. Cinnamomum camphora mainly has eucalyptol (EuL), camphor (CmR), linalool (LnL) and borneol (BeL) chemotypes basing on the uppermost monoterpenes. To reveal the thermotolerance mechanisms of these uppermost monoterpenes (eucalyptol, camphor, linalool, and borneol) in C. camphora, we surveyed the ROS metabolism and photosynthesis in the 4 chemotypes fumigated with the corresponding uppermost monoterpene after fosmidomycin (Fos) inhibiting monoterpene synthesis under high temperature at 38°C (Fos+38°C+monoterpene), and investigated the related gene expression in EuL and CmR. Meanwhile, the thermotolerance differences among the 4 uppermost monoterpenes were analyzed. In contrast to normal temperature (28°C), ROS levels and antioxidant enzyme activities in the 4 chemotypes increased under 38°C, and further increased in the treatment with Fos inhibiting monoterpene synthesis at 38°C (Fos+38°C), which may be caused by the alterations in expression of the genes related with non-enzymatic and enzymatic antioxidant formation according to the analyses in EuL and CmR. Compared with Fos+38°C treatment, Fos+38°C+monoterpene treatments lowered ROS levels and antioxidant enzyme activities for the increased non-enzymatic antioxidant gene expression and decreased enzymatic antioxidant gene expression, respectively. High temperature at 38°C reduced the chlorophyll and carotenoid content as well as photosynthetic abilities, which may result from the declined expression of the genes associated with photosynthetic pigment biosynthesis, light reaction, and carbon fixation. Fos+38°C treatment aggravated the reduction. In contrast to Fos+38°C treatment, Fos+38°C+monoterpene treatments increased photosynthetic pigment content and improved photosynthetic abilities by up-regulating related gene expression. Among the 4 uppermost monoterpenes, camphor showed strong abilities in lowering ROS and maintaining photosynthesis, while eucalyptol showed weak abilities. This was consistent with the recovery effects of the gene expression in the treatments with camphor and eucalyptol fumigation. Therefore, the uppermost monoterpenes can enhance C. camphora thermotolerance as signaling molecules, and may have differences in the signaling functions.https://www.frontiersin.org/articles/10.3389/fpls.2022.1072931/fullCinnamomum camphoragene expressionphotosynthesisreactive oxygen speciesthermotolerance mechanismuppermost monoterpene |
| spellingShingle | Chenyi Xu Chenyi Xu Bin Wang Bin Wang Qingyun Luo Qingyun Luo Yuandan Ma Yuandan Ma Tiefeng Zheng Tiefeng Zheng Yingying Wang Yingying Wang Yuyan Cai Yuyan Cai Zhaojiang Zuo Zhaojiang Zuo The uppermost monoterpenes improving Cinnamomum camphora thermotolerance by serving signaling functions Frontiers in Plant Science Cinnamomum camphora gene expression photosynthesis reactive oxygen species thermotolerance mechanism uppermost monoterpene |
| title | The uppermost monoterpenes improving Cinnamomum camphora thermotolerance by serving signaling functions |
| title_full | The uppermost monoterpenes improving Cinnamomum camphora thermotolerance by serving signaling functions |
| title_fullStr | The uppermost monoterpenes improving Cinnamomum camphora thermotolerance by serving signaling functions |
| title_full_unstemmed | The uppermost monoterpenes improving Cinnamomum camphora thermotolerance by serving signaling functions |
| title_short | The uppermost monoterpenes improving Cinnamomum camphora thermotolerance by serving signaling functions |
| title_sort | uppermost monoterpenes improving cinnamomum camphora thermotolerance by serving signaling functions |
| topic | Cinnamomum camphora gene expression photosynthesis reactive oxygen species thermotolerance mechanism uppermost monoterpene |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.1072931/full |
| work_keys_str_mv | AT chenyixu theuppermostmonoterpenesimprovingcinnamomumcamphorathermotolerancebyservingsignalingfunctions AT chenyixu theuppermostmonoterpenesimprovingcinnamomumcamphorathermotolerancebyservingsignalingfunctions AT binwang theuppermostmonoterpenesimprovingcinnamomumcamphorathermotolerancebyservingsignalingfunctions AT binwang theuppermostmonoterpenesimprovingcinnamomumcamphorathermotolerancebyservingsignalingfunctions AT qingyunluo theuppermostmonoterpenesimprovingcinnamomumcamphorathermotolerancebyservingsignalingfunctions AT qingyunluo theuppermostmonoterpenesimprovingcinnamomumcamphorathermotolerancebyservingsignalingfunctions AT yuandanma theuppermostmonoterpenesimprovingcinnamomumcamphorathermotolerancebyservingsignalingfunctions AT yuandanma theuppermostmonoterpenesimprovingcinnamomumcamphorathermotolerancebyservingsignalingfunctions AT tiefengzheng theuppermostmonoterpenesimprovingcinnamomumcamphorathermotolerancebyservingsignalingfunctions AT tiefengzheng theuppermostmonoterpenesimprovingcinnamomumcamphorathermotolerancebyservingsignalingfunctions AT yingyingwang theuppermostmonoterpenesimprovingcinnamomumcamphorathermotolerancebyservingsignalingfunctions AT yingyingwang theuppermostmonoterpenesimprovingcinnamomumcamphorathermotolerancebyservingsignalingfunctions AT yuyancai theuppermostmonoterpenesimprovingcinnamomumcamphorathermotolerancebyservingsignalingfunctions AT yuyancai theuppermostmonoterpenesimprovingcinnamomumcamphorathermotolerancebyservingsignalingfunctions AT zhaojiangzuo theuppermostmonoterpenesimprovingcinnamomumcamphorathermotolerancebyservingsignalingfunctions AT zhaojiangzuo theuppermostmonoterpenesimprovingcinnamomumcamphorathermotolerancebyservingsignalingfunctions AT chenyixu uppermostmonoterpenesimprovingcinnamomumcamphorathermotolerancebyservingsignalingfunctions AT chenyixu uppermostmonoterpenesimprovingcinnamomumcamphorathermotolerancebyservingsignalingfunctions AT binwang uppermostmonoterpenesimprovingcinnamomumcamphorathermotolerancebyservingsignalingfunctions AT binwang uppermostmonoterpenesimprovingcinnamomumcamphorathermotolerancebyservingsignalingfunctions AT qingyunluo uppermostmonoterpenesimprovingcinnamomumcamphorathermotolerancebyservingsignalingfunctions AT qingyunluo uppermostmonoterpenesimprovingcinnamomumcamphorathermotolerancebyservingsignalingfunctions AT yuandanma uppermostmonoterpenesimprovingcinnamomumcamphorathermotolerancebyservingsignalingfunctions AT yuandanma uppermostmonoterpenesimprovingcinnamomumcamphorathermotolerancebyservingsignalingfunctions AT tiefengzheng uppermostmonoterpenesimprovingcinnamomumcamphorathermotolerancebyservingsignalingfunctions AT tiefengzheng uppermostmonoterpenesimprovingcinnamomumcamphorathermotolerancebyservingsignalingfunctions AT yingyingwang uppermostmonoterpenesimprovingcinnamomumcamphorathermotolerancebyservingsignalingfunctions AT yingyingwang uppermostmonoterpenesimprovingcinnamomumcamphorathermotolerancebyservingsignalingfunctions AT yuyancai uppermostmonoterpenesimprovingcinnamomumcamphorathermotolerancebyservingsignalingfunctions AT yuyancai uppermostmonoterpenesimprovingcinnamomumcamphorathermotolerancebyservingsignalingfunctions AT zhaojiangzuo uppermostmonoterpenesimprovingcinnamomumcamphorathermotolerancebyservingsignalingfunctions AT zhaojiangzuo uppermostmonoterpenesimprovingcinnamomumcamphorathermotolerancebyservingsignalingfunctions |