Study of the Intrinsic Fluorescence of a Highly Branched Cationic Dendrimer, Poly(Ethyleneimine) (PEI)
Poly(ethyleneimine) (PEI) is a weakly basic, synthetic, polycationic polymer, due to the presence of primary, secondary, and tertiary amino groups. The amino groups are responsible for the variety of applications of PEI (e.g., transfection, bioimaging, solar cell, etc.). Our study presents some new...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-10-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/24/20/3690 |
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| author | Viktória Tóth Péter Hermann Dániel Végh Tivadar Zelles Zoltán Géczi |
| author_facet | Viktória Tóth Péter Hermann Dániel Végh Tivadar Zelles Zoltán Géczi |
| author_sort | Viktória Tóth |
| collection | DOAJ |
| description | Poly(ethyleneimine) (PEI) is a weakly basic, synthetic, polycationic polymer, due to the presence of primary, secondary, and tertiary amino groups. The amino groups are responsible for the variety of applications of PEI (e.g., transfection, bioimaging, solar cell, etc.). Our study presents some new and reproducible methods for the quantification of molecular or mass concentration of highly branched PEI of different molecular weights (800−2000−25,000−750,000 MW PEI). In the course of the direct method, spectrophotometry and fluorometry were applied to determine the absorption and fluorescence of PEI dilution series. An increase in the MW at the same concentration produces a higher count number because of the higher number of amino groups in PEI molecules. The character of increment in fluorescence intensity is essentially different in the case of mass concentrations and molar concentrations. The increment of the fluorescence intensity related to the molar concentration is non-linear. In the case of mass concentration, the slope is linear. Moreover, their fluorescence is enhanced with the decrease in pH values. The spectrophotometry is a reliable method for measuring the quantity of PEI molecules in solution. Our data help in recognizing the detailed properties of PEI in dendrimer research. |
| first_indexed | 2024-12-19T18:30:39Z |
| format | Article |
| id | doaj.art-2779534dfeda41bb8731d29ee6dedbdf |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-19T18:30:39Z |
| publishDate | 2019-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-2779534dfeda41bb8731d29ee6dedbdf2022-12-21T20:10:43ZengMDPI AGMolecules1420-30492019-10-012420369010.3390/molecules24203690molecules24203690Study of the Intrinsic Fluorescence of a Highly Branched Cationic Dendrimer, Poly(Ethyleneimine) (PEI)Viktória Tóth0Péter Hermann1Dániel Végh2Tivadar Zelles3Zoltán Géczi4Department of Prosthodontics, Semmelweis University, Szentkirályi u. 47, 1088 Budapest, HungaryDepartment of Prosthodontics, Semmelweis University, Szentkirályi u. 47, 1088 Budapest, HungaryDepartment of Prosthodontics, Semmelweis University, Szentkirályi u. 47, 1088 Budapest, HungaryDepartment of Oral Biology, Semmelweis University, Nagyvárad tér 4, 1089 Budapest, HungaryDepartment of Prosthodontics, Semmelweis University, Szentkirályi u. 47, 1088 Budapest, HungaryPoly(ethyleneimine) (PEI) is a weakly basic, synthetic, polycationic polymer, due to the presence of primary, secondary, and tertiary amino groups. The amino groups are responsible for the variety of applications of PEI (e.g., transfection, bioimaging, solar cell, etc.). Our study presents some new and reproducible methods for the quantification of molecular or mass concentration of highly branched PEI of different molecular weights (800−2000−25,000−750,000 MW PEI). In the course of the direct method, spectrophotometry and fluorometry were applied to determine the absorption and fluorescence of PEI dilution series. An increase in the MW at the same concentration produces a higher count number because of the higher number of amino groups in PEI molecules. The character of increment in fluorescence intensity is essentially different in the case of mass concentrations and molar concentrations. The increment of the fluorescence intensity related to the molar concentration is non-linear. In the case of mass concentration, the slope is linear. Moreover, their fluorescence is enhanced with the decrease in pH values. The spectrophotometry is a reliable method for measuring the quantity of PEI molecules in solution. Our data help in recognizing the detailed properties of PEI in dendrimer research.https://www.mdpi.com/1420-3049/24/20/3690biopolymersbiodegradabilityintrinsic fluorescencepolyethyleneiminedendrimermolecular weight |
| spellingShingle | Viktória Tóth Péter Hermann Dániel Végh Tivadar Zelles Zoltán Géczi Study of the Intrinsic Fluorescence of a Highly Branched Cationic Dendrimer, Poly(Ethyleneimine) (PEI) Molecules biopolymers biodegradability intrinsic fluorescence polyethyleneimine dendrimer molecular weight |
| title | Study of the Intrinsic Fluorescence of a Highly Branched Cationic Dendrimer, Poly(Ethyleneimine) (PEI) |
| title_full | Study of the Intrinsic Fluorescence of a Highly Branched Cationic Dendrimer, Poly(Ethyleneimine) (PEI) |
| title_fullStr | Study of the Intrinsic Fluorescence of a Highly Branched Cationic Dendrimer, Poly(Ethyleneimine) (PEI) |
| title_full_unstemmed | Study of the Intrinsic Fluorescence of a Highly Branched Cationic Dendrimer, Poly(Ethyleneimine) (PEI) |
| title_short | Study of the Intrinsic Fluorescence of a Highly Branched Cationic Dendrimer, Poly(Ethyleneimine) (PEI) |
| title_sort | study of the intrinsic fluorescence of a highly branched cationic dendrimer poly ethyleneimine pei |
| topic | biopolymers biodegradability intrinsic fluorescence polyethyleneimine dendrimer molecular weight |
| url | https://www.mdpi.com/1420-3049/24/20/3690 |
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