Antiparasitic potential of Nephrolepis biserrata methanol extract against the parasitic leech Zeylanicobdella arugamensis (Hirudinea) and LC-QTOF analysis
Abstract Marine leech Zeylanicobdella arugamensis (Piscicolidae), an economically important parasite is infesting predominantly cultured groupers, hybrid groupers and other fish in Southeast Asian countries. In this study, we tested the anti-parasitic potential of a medicinal plant Nephrolepis biser...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2020-12-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-020-79094-4 |
| _version_ | 1818432738603040768 |
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| author | Muhammad Dawood Shah Balu Alagar Venmathi Maran Fatin Khairah Haron Julian Ransangan Fui Fui Ching Sitti Raehanah Muhamad Shaleh Rossita Shapawi Yoong Soon Yong Susumu Ohtsuka |
| author_facet | Muhammad Dawood Shah Balu Alagar Venmathi Maran Fatin Khairah Haron Julian Ransangan Fui Fui Ching Sitti Raehanah Muhamad Shaleh Rossita Shapawi Yoong Soon Yong Susumu Ohtsuka |
| author_sort | Muhammad Dawood Shah |
| collection | DOAJ |
| description | Abstract Marine leech Zeylanicobdella arugamensis (Piscicolidae), an economically important parasite is infesting predominantly cultured groupers, hybrid groupers and other fish in Southeast Asian countries. In this study, we tested the anti-parasitic potential of a medicinal plant Nephrolepis biserrata found in Sabah, East Malaysia against Z. arugamensis. Various concentrations of methanol extracts of the plant were tested experimentally against Z. arugamensis and disinfestation of the leech from its primary host hybrid groupers. The composition of methanol extract of N. biserrata was determined through LC-QTOF analysis. The significant anti-parasitic activity of 100% mortality of leeches was observed with the exposure of N. biserrata extracts. The average time to kill the leeches at concentrations of 25, 50 and 100 mg/ml was 25.11 ± 3.26, 11.91 ± 0.99, and 4.88 ± 0.50 min., respectively. Further, at various low concentrations of N. biserrata 2.5, 5 and 10 mg/ml, hybrid groupers were disinfested in an average time of 108.33 ± 12.65, 65.83 ± 9.70 and 29.16 ± 5.85 min., respectively. The tandem mass spectrometry data from LC-QTOF indicated some hits on useful bioactive compounds such as terpenoids (ivalin, isovelleral, brassinolide, and eschscholtzxanthin), flavonoids (alnustin, kaempferol 7,4′-dimethyl ether, and pachypodol), phenolics (piscidic acid, chlorogenic acid, and ankorine), and aromatic (3-hydroxycoumarin). Thus N. biserrata can act as a potential biocontrol agent. |
| first_indexed | 2024-12-14T16:09:58Z |
| format | Article |
| id | doaj.art-277a71bd0fc045ac8d6ea2181ceeae3a |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-14T16:09:58Z |
| publishDate | 2020-12-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-277a71bd0fc045ac8d6ea2181ceeae3a2022-12-21T22:55:01ZengNature PortfolioScientific Reports2045-23222020-12-0110111010.1038/s41598-020-79094-4Antiparasitic potential of Nephrolepis biserrata methanol extract against the parasitic leech Zeylanicobdella arugamensis (Hirudinea) and LC-QTOF analysisMuhammad Dawood Shah0Balu Alagar Venmathi Maran1Fatin Khairah Haron2Julian Ransangan3Fui Fui Ching4Sitti Raehanah Muhamad Shaleh5Rossita Shapawi6Yoong Soon Yong7Susumu Ohtsuka8Borneo Marine Research Institute, Universiti Malaysia SabahBorneo Marine Research Institute, Universiti Malaysia SabahBorneo Marine Research Institute, Universiti Malaysia SabahBorneo Marine Research Institute, Universiti Malaysia SabahBorneo Marine Research Institute, Universiti Malaysia SabahBorneo Marine Research Institute, Universiti Malaysia SabahBorneo Marine Research Institute, Universiti Malaysia SabahXiamen University MalaysiaFisheries Science Laboratory, Setouchi Field Science Center, Graduate School of Integrated Sciences for Life, Hiroshima UniversityAbstract Marine leech Zeylanicobdella arugamensis (Piscicolidae), an economically important parasite is infesting predominantly cultured groupers, hybrid groupers and other fish in Southeast Asian countries. In this study, we tested the anti-parasitic potential of a medicinal plant Nephrolepis biserrata found in Sabah, East Malaysia against Z. arugamensis. Various concentrations of methanol extracts of the plant were tested experimentally against Z. arugamensis and disinfestation of the leech from its primary host hybrid groupers. The composition of methanol extract of N. biserrata was determined through LC-QTOF analysis. The significant anti-parasitic activity of 100% mortality of leeches was observed with the exposure of N. biserrata extracts. The average time to kill the leeches at concentrations of 25, 50 and 100 mg/ml was 25.11 ± 3.26, 11.91 ± 0.99, and 4.88 ± 0.50 min., respectively. Further, at various low concentrations of N. biserrata 2.5, 5 and 10 mg/ml, hybrid groupers were disinfested in an average time of 108.33 ± 12.65, 65.83 ± 9.70 and 29.16 ± 5.85 min., respectively. The tandem mass spectrometry data from LC-QTOF indicated some hits on useful bioactive compounds such as terpenoids (ivalin, isovelleral, brassinolide, and eschscholtzxanthin), flavonoids (alnustin, kaempferol 7,4′-dimethyl ether, and pachypodol), phenolics (piscidic acid, chlorogenic acid, and ankorine), and aromatic (3-hydroxycoumarin). Thus N. biserrata can act as a potential biocontrol agent.https://doi.org/10.1038/s41598-020-79094-4 |
| spellingShingle | Muhammad Dawood Shah Balu Alagar Venmathi Maran Fatin Khairah Haron Julian Ransangan Fui Fui Ching Sitti Raehanah Muhamad Shaleh Rossita Shapawi Yoong Soon Yong Susumu Ohtsuka Antiparasitic potential of Nephrolepis biserrata methanol extract against the parasitic leech Zeylanicobdella arugamensis (Hirudinea) and LC-QTOF analysis Scientific Reports |
| title | Antiparasitic potential of Nephrolepis biserrata methanol extract against the parasitic leech Zeylanicobdella arugamensis (Hirudinea) and LC-QTOF analysis |
| title_full | Antiparasitic potential of Nephrolepis biserrata methanol extract against the parasitic leech Zeylanicobdella arugamensis (Hirudinea) and LC-QTOF analysis |
| title_fullStr | Antiparasitic potential of Nephrolepis biserrata methanol extract against the parasitic leech Zeylanicobdella arugamensis (Hirudinea) and LC-QTOF analysis |
| title_full_unstemmed | Antiparasitic potential of Nephrolepis biserrata methanol extract against the parasitic leech Zeylanicobdella arugamensis (Hirudinea) and LC-QTOF analysis |
| title_short | Antiparasitic potential of Nephrolepis biserrata methanol extract against the parasitic leech Zeylanicobdella arugamensis (Hirudinea) and LC-QTOF analysis |
| title_sort | antiparasitic potential of nephrolepis biserrata methanol extract against the parasitic leech zeylanicobdella arugamensis hirudinea and lc qtof analysis |
| url | https://doi.org/10.1038/s41598-020-79094-4 |
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