Anticancer activity of Heat Shock Protein 70 (HSP70) Inhibitor, JG-98, against human cervical cancer HeLa and ovarian cancer SKOV-3 cells
Cervical and ovarian cancer are two aggressive neoplasms for women, still with high mortality and morbidity. Among the molecules and compounds that have anticancer activity, it was studied the JG-98, a heat shock protein 70 (HSP70) inhibitor. It demonstrated inhibitory effects on the growth of neopl...
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| Format: | Article |
| Language: | English |
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Society of Turaz Bilim
2023-03-01
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| Series: | Medicine Science |
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| Online Access: | https://www.medicinescience.org/?mno=133127 |
| _version_ | 1797316441296338944 |
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| author | Nazan Yurtcu Dilay Karademir Mustafa Ergul Ali Cetin Andrea Tinelli |
| author_facet | Nazan Yurtcu Dilay Karademir Mustafa Ergul Ali Cetin Andrea Tinelli |
| author_sort | Nazan Yurtcu |
| collection | DOAJ |
| description | Cervical and ovarian cancer are two aggressive neoplasms for women, still with high mortality and morbidity. Among the molecules and compounds that have anticancer activity, it was studied the JG-98, a heat shock protein 70 (HSP70) inhibitor. It demonstrated inhibitory effects on the growth of neoplastic cells, mediated by the induction of apoptosis, with anti-proliferation activity on neoplastic cells via the apoptotic pathway. The authors investigated the antiproliferative effects of JG-98 on human cervical cancer HeLa and ovarian cancer SKOV-3, examined by a standard XTT assay. Apoptotic effects and oxidative status were also evaluated by flow cytometry, ELISA, and total oxidant status assays in HeLa cells, respectively. The IC50 values of JG-98 in HeLa and SKOV-3 cells were recorded as 1.79 and 2.96 μM, respectively. Flow cytometry results showed that JG-98 treatment remarkably increased the proportion of apoptotic cells at IC50 concentration. The JG-98 treatment significantly increased the proteins Bax, cleaved caspase 3, cleaved PARP, and 8-oxo-dG levels, all indicators of cellular apoptosis. These findings show that JG-98 significantly decreased cell proliferation and increased apoptosis in HeLa cells, suggesting that JG-98 has a promising anti-tumor effect in cervical and ovarian cancers. [Med-Science 2023; 12(1.000): 20-5] |
| first_indexed | 2024-03-08T03:19:24Z |
| format | Article |
| id | doaj.art-277dc7e871b24322a3388d42c49d6f77 |
| institution | Directory Open Access Journal |
| issn | 2147-0634 |
| language | English |
| last_indexed | 2024-03-08T03:19:24Z |
| publishDate | 2023-03-01 |
| publisher | Society of Turaz Bilim |
| record_format | Article |
| series | Medicine Science |
| spelling | doaj.art-277dc7e871b24322a3388d42c49d6f772024-02-12T10:34:07ZengSociety of Turaz BilimMedicine Science2147-06342023-03-0112120510.5455/medscience.2022.11.251133127Anticancer activity of Heat Shock Protein 70 (HSP70) Inhibitor, JG-98, against human cervical cancer HeLa and ovarian cancer SKOV-3 cellsNazan Yurtcu0Dilay Karademir1Mustafa Ergul2Ali Cetin3Andrea Tinelli4Department of Obstetrics and Gynecology, Sivas Cumhuriyet University Faculty of Medicine, Sivas, Turkey Department of Obstetrics and Gynecology, Sivas Cumhuriyet University Faculty of Medicine, Sivas, Turkey Department of Biochemistry, Sivas Cumhuriyet University Faculty of Pharmacy, Sivas, Turkey Department of Obstetrics and Gynecology, Haseki Training and Research Hospital affiliated with the University of Health Sciences, Istanbul, Turkey Clinic of Obstetrics and Gynecology, Verris delli Ponti Hospital, Scorrano, Lecce, ItalyCervical and ovarian cancer are two aggressive neoplasms for women, still with high mortality and morbidity. Among the molecules and compounds that have anticancer activity, it was studied the JG-98, a heat shock protein 70 (HSP70) inhibitor. It demonstrated inhibitory effects on the growth of neoplastic cells, mediated by the induction of apoptosis, with anti-proliferation activity on neoplastic cells via the apoptotic pathway. The authors investigated the antiproliferative effects of JG-98 on human cervical cancer HeLa and ovarian cancer SKOV-3, examined by a standard XTT assay. Apoptotic effects and oxidative status were also evaluated by flow cytometry, ELISA, and total oxidant status assays in HeLa cells, respectively. The IC50 values of JG-98 in HeLa and SKOV-3 cells were recorded as 1.79 and 2.96 μM, respectively. Flow cytometry results showed that JG-98 treatment remarkably increased the proportion of apoptotic cells at IC50 concentration. The JG-98 treatment significantly increased the proteins Bax, cleaved caspase 3, cleaved PARP, and 8-oxo-dG levels, all indicators of cellular apoptosis. These findings show that JG-98 significantly decreased cell proliferation and increased apoptosis in HeLa cells, suggesting that JG-98 has a promising anti-tumor effect in cervical and ovarian cancers. [Med-Science 2023; 12(1.000): 20-5]https://www.medicinescience.org/?mno=133127cervical cancerovarian cancerhsp70jg-98hela cellsskov-3 cellsapoptosis |
| spellingShingle | Nazan Yurtcu Dilay Karademir Mustafa Ergul Ali Cetin Andrea Tinelli Anticancer activity of Heat Shock Protein 70 (HSP70) Inhibitor, JG-98, against human cervical cancer HeLa and ovarian cancer SKOV-3 cells Medicine Science cervical cancer ovarian cancer hsp70 jg-98 hela cells skov-3 cells apoptosis |
| title | Anticancer activity of Heat Shock Protein 70 (HSP70) Inhibitor, JG-98, against human cervical cancer HeLa and ovarian cancer SKOV-3 cells |
| title_full | Anticancer activity of Heat Shock Protein 70 (HSP70) Inhibitor, JG-98, against human cervical cancer HeLa and ovarian cancer SKOV-3 cells |
| title_fullStr | Anticancer activity of Heat Shock Protein 70 (HSP70) Inhibitor, JG-98, against human cervical cancer HeLa and ovarian cancer SKOV-3 cells |
| title_full_unstemmed | Anticancer activity of Heat Shock Protein 70 (HSP70) Inhibitor, JG-98, against human cervical cancer HeLa and ovarian cancer SKOV-3 cells |
| title_short | Anticancer activity of Heat Shock Protein 70 (HSP70) Inhibitor, JG-98, against human cervical cancer HeLa and ovarian cancer SKOV-3 cells |
| title_sort | anticancer activity of heat shock protein 70 hsp70 inhibitor jg 98 against human cervical cancer hela and ovarian cancer skov 3 cells |
| topic | cervical cancer ovarian cancer hsp70 jg-98 hela cells skov-3 cells apoptosis |
| url | https://www.medicinescience.org/?mno=133127 |
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