miR-765 as a promising biomarker for low-dose radiation-induced pulmonary fibrosis
High-dose radiation (HDR) is widely used for cancer treatment, but the effectiveness of low-dose radiation (LDR) in the treatment of various diseases is controversial. Therefore, to safely utilize LDR for therapeutic purposes, further research on its numerous biological effects of LDR is required. I...
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| Format: | Article |
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KeAi Communications Co., Ltd.
2024-03-01
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| Series: | Non-coding RNA Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2468054023000732 |
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| author | Hyun Jeong Seok Jae Yeon Choi Dong Hyeon Lee Joo Mi Yi Hae-June Lee In Hwa Bae |
| author_facet | Hyun Jeong Seok Jae Yeon Choi Dong Hyeon Lee Joo Mi Yi Hae-June Lee In Hwa Bae |
| author_sort | Hyun Jeong Seok |
| collection | DOAJ |
| description | High-dose radiation (HDR) is widely used for cancer treatment, but the effectiveness of low-dose radiation (LDR) in the treatment of various diseases is controversial. Therefore, to safely utilize LDR for therapeutic purposes, further research on its numerous biological effects of LDR is required. Interest in the increased use of medical imaging devices or the effects of surrounding living environmental radiation on the human body, particularly on fibrosis, is rapidly increasing. Therefore, this study aimed to verify the relationship between LDR and pulmonary fibrosis by evaluating the changes in fibroblasts after LDR treatment and their associated signaling mechanisms. LDR increased the expression of fibrosis markers COL1A1 and α-SMA, cell proliferation, and migration by activating YAP1 and Twist in fibroblasts. Meanwhile, miRNA was employed as a tool to inhibit LDR-induced fibrosis and it was found that miR-765 simultaneously targeted COL1A1, α-SMA, and YAP1. At the cellular level, miR-765 reduced the proliferation and migration of fibroblasts by suppressing the expression of LDR-induced fibrosis factors COL1A1, α-SMA, and YAP1. The efficacy of miR-765 in vivo was confirmed using bleomycin (BLM)-induced fibrotic mouse model. The characteristics of pulmonary fibrosis were reduced after injection of miR-765-overexpressing cells into BLM-induced fibrotic mice. In addition, the suppression of miR-765 expression in the plasma of patients with pulmonary fibrosis confirmed the negative relationship between pulmonary fibrosis and miR-765 expression. Therefore, this study demonstrates that miR-765 is a potential novel diagnostic biomarker and major target for the development of therapeutic agents to inhibit pulmonary fibrosis. |
| first_indexed | 2024-03-10T04:30:34Z |
| format | Article |
| id | doaj.art-277eaf5b471f4a92bcb7bbc442560061 |
| institution | Directory Open Access Journal |
| issn | 2468-0540 |
| language | English |
| last_indexed | 2024-04-25T01:12:17Z |
| publishDate | 2024-03-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Non-coding RNA Research |
| spelling | doaj.art-277eaf5b471f4a92bcb7bbc4425600612024-03-10T05:12:27ZengKeAi Communications Co., Ltd.Non-coding RNA Research2468-05402024-03-01913343miR-765 as a promising biomarker for low-dose radiation-induced pulmonary fibrosisHyun Jeong Seok0Jae Yeon Choi1Dong Hyeon Lee2Joo Mi Yi3Hae-June Lee4In Hwa Bae5Division of Radiation Biomedical Research, Korea Institute of Radiological & Medical Sciences, Seoul, Republic of Korea; Department of Life Science, Research Institute for Natural Sciences, Hanyang University, Seoul, Republic of KoreaDivision of Radiation Biomedical Research, Korea Institute of Radiological & Medical Sciences, Seoul, Republic of KoreaDivision of Radiation Biomedical Research, Korea Institute of Radiological & Medical Sciences, Seoul, Republic of KoreaDepartment of Microbiology and Immunology, College of Medicine, Inje University, Busan, Republic of KoreaDivision of Radiation Biomedical Research, Korea Institute of Radiological & Medical Sciences, Seoul, Republic of KoreaDivision of Radiation Biomedical Research, Korea Institute of Radiological & Medical Sciences, Seoul, Republic of Korea; Corresponding author.High-dose radiation (HDR) is widely used for cancer treatment, but the effectiveness of low-dose radiation (LDR) in the treatment of various diseases is controversial. Therefore, to safely utilize LDR for therapeutic purposes, further research on its numerous biological effects of LDR is required. Interest in the increased use of medical imaging devices or the effects of surrounding living environmental radiation on the human body, particularly on fibrosis, is rapidly increasing. Therefore, this study aimed to verify the relationship between LDR and pulmonary fibrosis by evaluating the changes in fibroblasts after LDR treatment and their associated signaling mechanisms. LDR increased the expression of fibrosis markers COL1A1 and α-SMA, cell proliferation, and migration by activating YAP1 and Twist in fibroblasts. Meanwhile, miRNA was employed as a tool to inhibit LDR-induced fibrosis and it was found that miR-765 simultaneously targeted COL1A1, α-SMA, and YAP1. At the cellular level, miR-765 reduced the proliferation and migration of fibroblasts by suppressing the expression of LDR-induced fibrosis factors COL1A1, α-SMA, and YAP1. The efficacy of miR-765 in vivo was confirmed using bleomycin (BLM)-induced fibrotic mouse model. The characteristics of pulmonary fibrosis were reduced after injection of miR-765-overexpressing cells into BLM-induced fibrotic mice. In addition, the suppression of miR-765 expression in the plasma of patients with pulmonary fibrosis confirmed the negative relationship between pulmonary fibrosis and miR-765 expression. Therefore, this study demonstrates that miR-765 is a potential novel diagnostic biomarker and major target for the development of therapeutic agents to inhibit pulmonary fibrosis.http://www.sciencedirect.com/science/article/pii/S2468054023000732Low dose radiationPulmonary fibrosismiR-765BiomarkerYAP1 |
| spellingShingle | Hyun Jeong Seok Jae Yeon Choi Dong Hyeon Lee Joo Mi Yi Hae-June Lee In Hwa Bae miR-765 as a promising biomarker for low-dose radiation-induced pulmonary fibrosis Non-coding RNA Research Low dose radiation Pulmonary fibrosis miR-765 Biomarker YAP1 |
| title | miR-765 as a promising biomarker for low-dose radiation-induced pulmonary fibrosis |
| title_full | miR-765 as a promising biomarker for low-dose radiation-induced pulmonary fibrosis |
| title_fullStr | miR-765 as a promising biomarker for low-dose radiation-induced pulmonary fibrosis |
| title_full_unstemmed | miR-765 as a promising biomarker for low-dose radiation-induced pulmonary fibrosis |
| title_short | miR-765 as a promising biomarker for low-dose radiation-induced pulmonary fibrosis |
| title_sort | mir 765 as a promising biomarker for low dose radiation induced pulmonary fibrosis |
| topic | Low dose radiation Pulmonary fibrosis miR-765 Biomarker YAP1 |
| url | http://www.sciencedirect.com/science/article/pii/S2468054023000732 |
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