The VELVET Complex in the Gray Mold Fungus Botrytis cinerea: Impact of BcLAE1 on Differentiation, Secondary Metabolism, and Virulence
Botrytis cinerea, the gray mold fungus, is an important plant pathogen. Field populations are characterized by variability with regard to morphology, the mode of reproduction (conidiation or sclerotia formation), the spectrum of secondary metabolites (SM), and virulence. Natural variation in bcvel1...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
2015-06-01
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| Series: | Molecular Plant-Microbe Interactions |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI-12-14-0411-R |
| _version_ | 1811261351970996224 |
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| author | Julia Schumacher Adeline Simon Kim C. Cohrs Stefanie Traeger Antoine Porquier Bérengère Dalmais Muriel Viaud Bettina Tudzynski |
| author_facet | Julia Schumacher Adeline Simon Kim C. Cohrs Stefanie Traeger Antoine Porquier Bérengère Dalmais Muriel Viaud Bettina Tudzynski |
| author_sort | Julia Schumacher |
| collection | DOAJ |
| description | Botrytis cinerea, the gray mold fungus, is an important plant pathogen. Field populations are characterized by variability with regard to morphology, the mode of reproduction (conidiation or sclerotia formation), the spectrum of secondary metabolites (SM), and virulence. Natural variation in bcvel1 encoding the ortholog of Aspergillus nidulans VeA, a member of the VELVET complex, was previously shown to affect light-dependent differentiation, the formation of oxalic acid (OA), and virulence. To gain broader insight into the B. cinerea VELVET complex, an ortholog of A. nidulans LaeA, BcLAE1, a putative interaction partner of BcVEL1, was studied. BcVEL1 but not its truncated versions interacts with BcLAE1 and BcVEL2 (VelB ortholog). In accordance with the expected common as well as specific functions of BcVEL1 and BcLAE1, the deletions of both genes result in similar though not identical phenotypes. Both mutants lost the ability to produce OA, to colonize the host tissue, and to form sclerotia. However, mutants differ with regard to aerial hyphae and conidia formation. Genome-wide expression analyses revealed that BcVEL1 and BcLAE1 have common and distinct target genes. Some of the genes that are underexpressed in both mutants, e.g., those encoding SM-related enzymes, proteases, and carbohydrate-active enzymes, may account for their reduced virulence. |
| first_indexed | 2024-04-12T19:01:54Z |
| format | Article |
| id | doaj.art-277ec764368b4587b4def74d6bd7c398 |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-04-12T19:01:54Z |
| publishDate | 2015-06-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-277ec764368b4587b4def74d6bd7c3982022-12-22T03:20:07ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062015-06-0128665967410.1094/MPMI-12-14-0411-RThe VELVET Complex in the Gray Mold Fungus Botrytis cinerea: Impact of BcLAE1 on Differentiation, Secondary Metabolism, and VirulenceJulia Schumacher0Adeline Simon1Kim C. Cohrs2Stefanie Traeger3Antoine Porquier4Bérengère Dalmais5Muriel Viaud6Bettina Tudzynski7IBBP, WWU Münster, Schlossplatz 8, 48143 Münster, Germany;BIOGER, INRA, Avenue Lucien Brétignières, 78850 Grignon, France;IBBP, WWU Münster, Schlossplatz 8, 48143 Münster, Germany;IBBP, WWU Münster, Schlossplatz 8, 48143 Münster, Germany;BIOGER, INRA, Avenue Lucien Brétignières, 78850 Grignon, France;BIOGER, INRA, Avenue Lucien Brétignières, 78850 Grignon, France;BIOGER, INRA, Avenue Lucien Brétignières, 78850 Grignon, France;IBBP, WWU Münster, Schlossplatz 8, 48143 Münster, Germany;Botrytis cinerea, the gray mold fungus, is an important plant pathogen. Field populations are characterized by variability with regard to morphology, the mode of reproduction (conidiation or sclerotia formation), the spectrum of secondary metabolites (SM), and virulence. Natural variation in bcvel1 encoding the ortholog of Aspergillus nidulans VeA, a member of the VELVET complex, was previously shown to affect light-dependent differentiation, the formation of oxalic acid (OA), and virulence. To gain broader insight into the B. cinerea VELVET complex, an ortholog of A. nidulans LaeA, BcLAE1, a putative interaction partner of BcVEL1, was studied. BcVEL1 but not its truncated versions interacts with BcLAE1 and BcVEL2 (VelB ortholog). In accordance with the expected common as well as specific functions of BcVEL1 and BcLAE1, the deletions of both genes result in similar though not identical phenotypes. Both mutants lost the ability to produce OA, to colonize the host tissue, and to form sclerotia. However, mutants differ with regard to aerial hyphae and conidia formation. Genome-wide expression analyses revealed that BcVEL1 and BcLAE1 have common and distinct target genes. Some of the genes that are underexpressed in both mutants, e.g., those encoding SM-related enzymes, proteases, and carbohydrate-active enzymes, may account for their reduced virulence.https://apsjournals.apsnet.org/doi/10.1094/MPMI-12-14-0411-R |
| spellingShingle | Julia Schumacher Adeline Simon Kim C. Cohrs Stefanie Traeger Antoine Porquier Bérengère Dalmais Muriel Viaud Bettina Tudzynski The VELVET Complex in the Gray Mold Fungus Botrytis cinerea: Impact of BcLAE1 on Differentiation, Secondary Metabolism, and Virulence Molecular Plant-Microbe Interactions |
| title | The VELVET Complex in the Gray Mold Fungus Botrytis cinerea: Impact of BcLAE1 on Differentiation, Secondary Metabolism, and Virulence |
| title_full | The VELVET Complex in the Gray Mold Fungus Botrytis cinerea: Impact of BcLAE1 on Differentiation, Secondary Metabolism, and Virulence |
| title_fullStr | The VELVET Complex in the Gray Mold Fungus Botrytis cinerea: Impact of BcLAE1 on Differentiation, Secondary Metabolism, and Virulence |
| title_full_unstemmed | The VELVET Complex in the Gray Mold Fungus Botrytis cinerea: Impact of BcLAE1 on Differentiation, Secondary Metabolism, and Virulence |
| title_short | The VELVET Complex in the Gray Mold Fungus Botrytis cinerea: Impact of BcLAE1 on Differentiation, Secondary Metabolism, and Virulence |
| title_sort | velvet complex in the gray mold fungus botrytis cinerea impact of bclae1 on differentiation secondary metabolism and virulence |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI-12-14-0411-R |
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