<i>Garcinia dulcis</i> Flower Extract Alters Gut Microbiota and Fecal Metabolomic Profiles of 2K1C Hypertensive Rats
<i>Garcinia dulcis</i> (GD) extract has been found to have anti-hypertensive properties in animal studies. GD can also alter the colonic microbiota of rats. However, the effects of GD on changes in the gut microbiota and metabolomic profiles of normotensive and hypertensive rats are curr...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-01-01
|
| Series: | Nutrients |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2072-6643/15/2/268 |
| _version_ | 1797438099811205120 |
|---|---|
| author | Phornphan Sornchuer Nattaya Thongsepee Lampet Wongsaroj Kritsakorn Saninjuk Suphot Wattanaphansak Pornkanok Pongpamorn Atchara Paemanee Pongsakorn Martviset Pathanin Chantree Kant Sangpairoj |
| author_facet | Phornphan Sornchuer Nattaya Thongsepee Lampet Wongsaroj Kritsakorn Saninjuk Suphot Wattanaphansak Pornkanok Pongpamorn Atchara Paemanee Pongsakorn Martviset Pathanin Chantree Kant Sangpairoj |
| author_sort | Phornphan Sornchuer |
| collection | DOAJ |
| description | <i>Garcinia dulcis</i> (GD) extract has been found to have anti-hypertensive properties in animal studies. GD can also alter the colonic microbiota of rats. However, the effects of GD on changes in the gut microbiota and metabolomic profiles of normotensive and hypertensive rats are currently unknown. The purpose of this study was to evaluate changes in the gut microbiota and metabolomic profiles of 2-kidneys-1 clip (2K1C) hypertensive rats after feeding with GD flower extract. Rats were randomly divided into the following 4 groups: sham operation (SO) receiving corn oil (CO) (SO + CO), SO receiving GD (SO + GD), 2K1C receiving corn oil (2K1C + CO) and 2K1C receiving GD (2K1C + GD). Body weight (BW) and systolic blood pressure (SBP) were measured weekly throughout the study. Gut microbiota and fecal metabolites were measured from fresh fecal contents. Alpha diversity results demonstrated a similar microbial richness and diversity between groups. Linear discriminant analysis (LDA) effect size (LEfSe) suggested that GD treatment affected gut microbial community structure in both hypertensive and normotensive rats. Feeding rats with GD caused metabolic alterations that rendered 2K1C + GD rats similar to SO + CO and SO + GD rats. Findings suggest that the impact of GD on gut microbiota and metabolite profiles may be related to its anti-hypertensive properties. |
| first_indexed | 2024-03-09T11:32:22Z |
| format | Article |
| id | doaj.art-278096ecca8c40639934817def70f7d8 |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-09T11:32:22Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-278096ecca8c40639934817def70f7d82023-11-30T23:49:12ZengMDPI AGNutrients2072-66432023-01-0115226810.3390/nu15020268<i>Garcinia dulcis</i> Flower Extract Alters Gut Microbiota and Fecal Metabolomic Profiles of 2K1C Hypertensive RatsPhornphan Sornchuer0Nattaya Thongsepee1Lampet Wongsaroj2Kritsakorn Saninjuk3Suphot Wattanaphansak4Pornkanok Pongpamorn5Atchara Paemanee6Pongsakorn Martviset7Pathanin Chantree8Kant Sangpairoj9Department of Preclinical Science, Faculty of Medicine, Thammasat University, Khlong Luang 12120, ThailandDepartment of Preclinical Science, Faculty of Medicine, Thammasat University, Khlong Luang 12120, ThailandPorcinotec Co., Ltd., Nonthaburi 11000, ThailandPorcinotec Co., Ltd., Nonthaburi 11000, ThailandDepartments of Veterinary Medicine, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, ThailandNational Omics Center, National Science and Technology Development Agency (NSTDA), Khlong Luang 12120, ThailandNational Omics Center, National Science and Technology Development Agency (NSTDA), Khlong Luang 12120, ThailandDepartment of Preclinical Science, Faculty of Medicine, Thammasat University, Khlong Luang 12120, ThailandDepartment of Preclinical Science, Faculty of Medicine, Thammasat University, Khlong Luang 12120, ThailandDepartment of Preclinical Science, Faculty of Medicine, Thammasat University, Khlong Luang 12120, Thailand<i>Garcinia dulcis</i> (GD) extract has been found to have anti-hypertensive properties in animal studies. GD can also alter the colonic microbiota of rats. However, the effects of GD on changes in the gut microbiota and metabolomic profiles of normotensive and hypertensive rats are currently unknown. The purpose of this study was to evaluate changes in the gut microbiota and metabolomic profiles of 2-kidneys-1 clip (2K1C) hypertensive rats after feeding with GD flower extract. Rats were randomly divided into the following 4 groups: sham operation (SO) receiving corn oil (CO) (SO + CO), SO receiving GD (SO + GD), 2K1C receiving corn oil (2K1C + CO) and 2K1C receiving GD (2K1C + GD). Body weight (BW) and systolic blood pressure (SBP) were measured weekly throughout the study. Gut microbiota and fecal metabolites were measured from fresh fecal contents. Alpha diversity results demonstrated a similar microbial richness and diversity between groups. Linear discriminant analysis (LDA) effect size (LEfSe) suggested that GD treatment affected gut microbial community structure in both hypertensive and normotensive rats. Feeding rats with GD caused metabolic alterations that rendered 2K1C + GD rats similar to SO + CO and SO + GD rats. Findings suggest that the impact of GD on gut microbiota and metabolite profiles may be related to its anti-hypertensive properties.https://www.mdpi.com/2072-6643/15/2/268gut microbiotametabolites2K1C rat<i>Garcinia dulcis</i>hypertension |
| spellingShingle | Phornphan Sornchuer Nattaya Thongsepee Lampet Wongsaroj Kritsakorn Saninjuk Suphot Wattanaphansak Pornkanok Pongpamorn Atchara Paemanee Pongsakorn Martviset Pathanin Chantree Kant Sangpairoj <i>Garcinia dulcis</i> Flower Extract Alters Gut Microbiota and Fecal Metabolomic Profiles of 2K1C Hypertensive Rats Nutrients gut microbiota metabolites 2K1C rat <i>Garcinia dulcis</i> hypertension |
| title | <i>Garcinia dulcis</i> Flower Extract Alters Gut Microbiota and Fecal Metabolomic Profiles of 2K1C Hypertensive Rats |
| title_full | <i>Garcinia dulcis</i> Flower Extract Alters Gut Microbiota and Fecal Metabolomic Profiles of 2K1C Hypertensive Rats |
| title_fullStr | <i>Garcinia dulcis</i> Flower Extract Alters Gut Microbiota and Fecal Metabolomic Profiles of 2K1C Hypertensive Rats |
| title_full_unstemmed | <i>Garcinia dulcis</i> Flower Extract Alters Gut Microbiota and Fecal Metabolomic Profiles of 2K1C Hypertensive Rats |
| title_short | <i>Garcinia dulcis</i> Flower Extract Alters Gut Microbiota and Fecal Metabolomic Profiles of 2K1C Hypertensive Rats |
| title_sort | i garcinia dulcis i flower extract alters gut microbiota and fecal metabolomic profiles of 2k1c hypertensive rats |
| topic | gut microbiota metabolites 2K1C rat <i>Garcinia dulcis</i> hypertension |
| url | https://www.mdpi.com/2072-6643/15/2/268 |
| work_keys_str_mv | AT phornphansornchuer igarciniadulcisiflowerextractaltersgutmicrobiotaandfecalmetabolomicprofilesof2k1chypertensiverats AT nattayathongsepee igarciniadulcisiflowerextractaltersgutmicrobiotaandfecalmetabolomicprofilesof2k1chypertensiverats AT lampetwongsaroj igarciniadulcisiflowerextractaltersgutmicrobiotaandfecalmetabolomicprofilesof2k1chypertensiverats AT kritsakornsaninjuk igarciniadulcisiflowerextractaltersgutmicrobiotaandfecalmetabolomicprofilesof2k1chypertensiverats AT suphotwattanaphansak igarciniadulcisiflowerextractaltersgutmicrobiotaandfecalmetabolomicprofilesof2k1chypertensiverats AT pornkanokpongpamorn igarciniadulcisiflowerextractaltersgutmicrobiotaandfecalmetabolomicprofilesof2k1chypertensiverats AT atcharapaemanee igarciniadulcisiflowerextractaltersgutmicrobiotaandfecalmetabolomicprofilesof2k1chypertensiverats AT pongsakornmartviset igarciniadulcisiflowerextractaltersgutmicrobiotaandfecalmetabolomicprofilesof2k1chypertensiverats AT pathaninchantree igarciniadulcisiflowerextractaltersgutmicrobiotaandfecalmetabolomicprofilesof2k1chypertensiverats AT kantsangpairoj igarciniadulcisiflowerextractaltersgutmicrobiotaandfecalmetabolomicprofilesof2k1chypertensiverats |