Genomic characterization of Wenzhou mammarenavirus detected in wild rodents in Guangzhou City, China
Wenzhou mammarenavirus (WENV) is a zoonotic pathogen newly discovered in east and southeast Asia. WENV has been found in wild rodent animals around the world while its standing is barely understood in Guangzhou city, where is known as a region of outbreak hotspot for zoonotic emerging infectious dis...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-12-01
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| Series: | One Health |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S235277142100063X |
| _version_ | 1819096890957889536 |
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| author | Jian-Yong Wu Cheng Guo Yao Xia Hui-Min Bao Yan-Shan Zhu Zhong-Min Guo Yue-Hong Wei Jia-Hai Lu |
| author_facet | Jian-Yong Wu Cheng Guo Yao Xia Hui-Min Bao Yan-Shan Zhu Zhong-Min Guo Yue-Hong Wei Jia-Hai Lu |
| author_sort | Jian-Yong Wu |
| collection | DOAJ |
| description | Wenzhou mammarenavirus (WENV) is a zoonotic pathogen newly discovered in east and southeast Asia. WENV has been found in wild rodent animals around the world while its standing is barely understood in Guangzhou city, where is known as a region of outbreak hotspot for zoonotic emerging infectious diseases. To investigate the prevalence and genomic characteristics of mammarenavirus in Guangzhou City, lung tissue samples from wild rodent species were collected from five districts of Guangzhou City in the year 2015 and 2016. The viral RNA was extracted and then subjected to mammarenavirus-specific PCR. The result revealed approximately 1.0% (3/306) nucleic acid positivity for lung tissue samples obtained from three rodent species: Mus musculus, Rattus flavipectus, and Rattus norvegicus. Viral metagenomic sequencing of three samples was then carried out and two full segment L and three full segment S sequences were obtained. Phylogenetics analysis indicated the sequences of the new mammarenavirus strain have 76.2% - 94.4% similarity to known WENV encoded genes, with the highest similarity to the WENV 9–24 strain. Population structure analysis grouped all known WENV into seven lineages, and this WENV Guangzhou strain was grouped with WENV 9–24 as well. Though the seroprevalence result was not available, our data provides the first nucleic acid evidence of circulating WENV in Guangzhou city, and it suggested WENV had a broader host tropism than previously known. |
| first_indexed | 2024-12-22T00:06:23Z |
| format | Article |
| id | doaj.art-27817e2a250b495491feb29bd64537a4 |
| institution | Directory Open Access Journal |
| issn | 2352-7714 |
| language | English |
| last_indexed | 2024-12-22T00:06:23Z |
| publishDate | 2021-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | One Health |
| spelling | doaj.art-27817e2a250b495491feb29bd64537a42022-12-21T18:45:33ZengElsevierOne Health2352-77142021-12-0113100273Genomic characterization of Wenzhou mammarenavirus detected in wild rodents in Guangzhou City, ChinaJian-Yong Wu0Cheng Guo1Yao Xia2Hui-Min Bao3Yan-Shan Zhu4Zhong-Min Guo5Yue-Hong Wei6Jia-Hai Lu7School of Public Health, Sun Yat-Sen University, Guangzhou 510080, ChinaCenter for Infection and Immunity, Mailman School of Public Health, Columbia University, New York 10032, USASchool of Public Health, Sun Yat-Sen University, Guangzhou 510080, China; Kunming Institute of Zoology, Chinese Acadamy of Sciences, Kunming 650201, ChinaSchool of Public Health, Sun Yat-Sen University, Guangzhou 510080, ChinaSchool of Public Health, Sun Yat-Sen University, Guangzhou 510080, ChinaLaboratory Animal Center, Sun Yat-Sen University, Guangzhou 510080, ChinaGuangzhou Center for Disease Control and Prevention, Guangzhou 510440, China; Corresponding author at: Guangzhou Center for Disease Control and Prevention, Guangzhou 510440, China.School of Public Health, Sun Yat-Sen University, Guangzhou 510080, China; One Health Center of Excellence for Research & Training, Sun Yat-Sen University, Guangzhou 510080, China; Key Laboratory for Tropical Disease Control of Ministry of Education, Sun Yat-Sen University, Guangzhou 510080, China; NMPA Key Laboratory for Quality Monitoring and Evaluation of Vaccines and Biological Products, Sun Yat-Sen University, Guangzhou 510080, China; Corresponding author at: School of Public Health, Sun Yat-Sen University, Guangzhou 510080, China.Wenzhou mammarenavirus (WENV) is a zoonotic pathogen newly discovered in east and southeast Asia. WENV has been found in wild rodent animals around the world while its standing is barely understood in Guangzhou city, where is known as a region of outbreak hotspot for zoonotic emerging infectious diseases. To investigate the prevalence and genomic characteristics of mammarenavirus in Guangzhou City, lung tissue samples from wild rodent species were collected from five districts of Guangzhou City in the year 2015 and 2016. The viral RNA was extracted and then subjected to mammarenavirus-specific PCR. The result revealed approximately 1.0% (3/306) nucleic acid positivity for lung tissue samples obtained from three rodent species: Mus musculus, Rattus flavipectus, and Rattus norvegicus. Viral metagenomic sequencing of three samples was then carried out and two full segment L and three full segment S sequences were obtained. Phylogenetics analysis indicated the sequences of the new mammarenavirus strain have 76.2% - 94.4% similarity to known WENV encoded genes, with the highest similarity to the WENV 9–24 strain. Population structure analysis grouped all known WENV into seven lineages, and this WENV Guangzhou strain was grouped with WENV 9–24 as well. Though the seroprevalence result was not available, our data provides the first nucleic acid evidence of circulating WENV in Guangzhou city, and it suggested WENV had a broader host tropism than previously known.http://www.sciencedirect.com/science/article/pii/S235277142100063XWenzhou mammarenavirusGenomic characteristicsWild rodentPhylogenetic analysisPopulation structureGuangzhou city |
| spellingShingle | Jian-Yong Wu Cheng Guo Yao Xia Hui-Min Bao Yan-Shan Zhu Zhong-Min Guo Yue-Hong Wei Jia-Hai Lu Genomic characterization of Wenzhou mammarenavirus detected in wild rodents in Guangzhou City, China One Health Wenzhou mammarenavirus Genomic characteristics Wild rodent Phylogenetic analysis Population structure Guangzhou city |
| title | Genomic characterization of Wenzhou mammarenavirus detected in wild rodents in Guangzhou City, China |
| title_full | Genomic characterization of Wenzhou mammarenavirus detected in wild rodents in Guangzhou City, China |
| title_fullStr | Genomic characterization of Wenzhou mammarenavirus detected in wild rodents in Guangzhou City, China |
| title_full_unstemmed | Genomic characterization of Wenzhou mammarenavirus detected in wild rodents in Guangzhou City, China |
| title_short | Genomic characterization of Wenzhou mammarenavirus detected in wild rodents in Guangzhou City, China |
| title_sort | genomic characterization of wenzhou mammarenavirus detected in wild rodents in guangzhou city china |
| topic | Wenzhou mammarenavirus Genomic characteristics Wild rodent Phylogenetic analysis Population structure Guangzhou city |
| url | http://www.sciencedirect.com/science/article/pii/S235277142100063X |
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