Podocyte-specific deletion of tubular sclerosis complex 2 promotes focal segmental glomerulosclerosis and progressive renal failure.
Obesity can initiate and accelerate the progression of kidney diseases. However, it remains unclear how obesity affects renal dysfunction. Here, we show that a newly generated podocyte-specific tubular sclerosis complex 2 (Tsc2) knockout mouse model (Tsc2Δpodocyte) develops proteinuria and dies due...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2020-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0229397 |
| _version_ | 1818473640681799680 |
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| author | Wakiko Iwata Hiroyuki Unoki-Kubota Hideki Kato Akira Shimizu Michihiro Matsumoto Toshiyuki Imasawa Arisa Igarashi Kenji Matsumoto Tetsuo Noda Yasuo Terauchi Masaomi Nangaku Masato Kasuga Yasushi Kaburagi |
| author_facet | Wakiko Iwata Hiroyuki Unoki-Kubota Hideki Kato Akira Shimizu Michihiro Matsumoto Toshiyuki Imasawa Arisa Igarashi Kenji Matsumoto Tetsuo Noda Yasuo Terauchi Masaomi Nangaku Masato Kasuga Yasushi Kaburagi |
| author_sort | Wakiko Iwata |
| collection | DOAJ |
| description | Obesity can initiate and accelerate the progression of kidney diseases. However, it remains unclear how obesity affects renal dysfunction. Here, we show that a newly generated podocyte-specific tubular sclerosis complex 2 (Tsc2) knockout mouse model (Tsc2Δpodocyte) develops proteinuria and dies due to end-stage renal dysfunction by 10 weeks of age. Tsc2Δpodocyte mice exhibit an increased glomerular size and focal segmental glomerulosclerosis, including podocyte foot process effacement, mesangial sclerosis and proteinaceous casts. Podocytes isolated from Tsc2Δpodocyte mice show nuclear factor, erythroid derived 2, like 2-mediated increased oxidative stress response on microarray analysis and their autophagic activity is lowered through the mammalian target of rapamycin (mTOR)-unc-51-like kinase 1 pathway. Rapamycin attenuated podocyte dysfunction and extends survival in Tsc2Δpodocyte mice. Additionally, mTOR complex 1 (mTORC1) activity is increased in podocytes of renal biopsy specimens obtained from obese patients with chronic kidney disease. Our work shows that mTORC1 hyperactivation in podocytes leads to severe renal dysfunction and that inhibition of mTORC1 activity in podocytes could be a key therapeutic target for obesity-related kidney diseases. |
| first_indexed | 2024-04-14T04:24:54Z |
| format | Article |
| id | doaj.art-2781a5cf89ec49e594b7cf2923e6bc38 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-14T04:24:54Z |
| publishDate | 2020-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-2781a5cf89ec49e594b7cf2923e6bc382022-12-22T02:12:19ZengPublic Library of Science (PLoS)PLoS ONE1932-62032020-01-01153e022939710.1371/journal.pone.0229397Podocyte-specific deletion of tubular sclerosis complex 2 promotes focal segmental glomerulosclerosis and progressive renal failure.Wakiko IwataHiroyuki Unoki-KubotaHideki KatoAkira ShimizuMichihiro MatsumotoToshiyuki ImasawaArisa IgarashiKenji MatsumotoTetsuo NodaYasuo TerauchiMasaomi NangakuMasato KasugaYasushi KaburagiObesity can initiate and accelerate the progression of kidney diseases. However, it remains unclear how obesity affects renal dysfunction. Here, we show that a newly generated podocyte-specific tubular sclerosis complex 2 (Tsc2) knockout mouse model (Tsc2Δpodocyte) develops proteinuria and dies due to end-stage renal dysfunction by 10 weeks of age. Tsc2Δpodocyte mice exhibit an increased glomerular size and focal segmental glomerulosclerosis, including podocyte foot process effacement, mesangial sclerosis and proteinaceous casts. Podocytes isolated from Tsc2Δpodocyte mice show nuclear factor, erythroid derived 2, like 2-mediated increased oxidative stress response on microarray analysis and their autophagic activity is lowered through the mammalian target of rapamycin (mTOR)-unc-51-like kinase 1 pathway. Rapamycin attenuated podocyte dysfunction and extends survival in Tsc2Δpodocyte mice. Additionally, mTOR complex 1 (mTORC1) activity is increased in podocytes of renal biopsy specimens obtained from obese patients with chronic kidney disease. Our work shows that mTORC1 hyperactivation in podocytes leads to severe renal dysfunction and that inhibition of mTORC1 activity in podocytes could be a key therapeutic target for obesity-related kidney diseases.https://doi.org/10.1371/journal.pone.0229397 |
| spellingShingle | Wakiko Iwata Hiroyuki Unoki-Kubota Hideki Kato Akira Shimizu Michihiro Matsumoto Toshiyuki Imasawa Arisa Igarashi Kenji Matsumoto Tetsuo Noda Yasuo Terauchi Masaomi Nangaku Masato Kasuga Yasushi Kaburagi Podocyte-specific deletion of tubular sclerosis complex 2 promotes focal segmental glomerulosclerosis and progressive renal failure. PLoS ONE |
| title | Podocyte-specific deletion of tubular sclerosis complex 2 promotes focal segmental glomerulosclerosis and progressive renal failure. |
| title_full | Podocyte-specific deletion of tubular sclerosis complex 2 promotes focal segmental glomerulosclerosis and progressive renal failure. |
| title_fullStr | Podocyte-specific deletion of tubular sclerosis complex 2 promotes focal segmental glomerulosclerosis and progressive renal failure. |
| title_full_unstemmed | Podocyte-specific deletion of tubular sclerosis complex 2 promotes focal segmental glomerulosclerosis and progressive renal failure. |
| title_short | Podocyte-specific deletion of tubular sclerosis complex 2 promotes focal segmental glomerulosclerosis and progressive renal failure. |
| title_sort | podocyte specific deletion of tubular sclerosis complex 2 promotes focal segmental glomerulosclerosis and progressive renal failure |
| url | https://doi.org/10.1371/journal.pone.0229397 |
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