Treated dentin matrix induces odontogenic differentiation of dental pulp stem cells via regulation of Wnt/β-catenin signaling
Treated dentin matrix (TDM) is an ideal scaffold material containing multiple extracellular matrix factors. The canonical Wnt signaling pathway is necessary for tooth regeneration. Thus, this study investigated whether the TDM can promote the odontogenic differentiation of human dental pulp stem cel...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2022-01-01
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| Series: | Bioactive Materials |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2452199X21002474 |
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| author | Sirui Liu Jingjing Sun Shuai Yuan Yanyu Yang Yuping Gong Ying Wang Runying Guo Xue Zhang Yiming Liu Hongyan Mi Meiyue Wang Mengzhe Liu Rui Li |
| author_facet | Sirui Liu Jingjing Sun Shuai Yuan Yanyu Yang Yuping Gong Ying Wang Runying Guo Xue Zhang Yiming Liu Hongyan Mi Meiyue Wang Mengzhe Liu Rui Li |
| author_sort | Sirui Liu |
| collection | DOAJ |
| description | Treated dentin matrix (TDM) is an ideal scaffold material containing multiple extracellular matrix factors. The canonical Wnt signaling pathway is necessary for tooth regeneration. Thus, this study investigated whether the TDM can promote the odontogenic differentiation of human dental pulp stem cells (hDPSCs) and determined the potential role of Wnt/β-catenin signaling in this process. Different concentrations of TDM promoted the dental differentiation of the hDPSCs and meanwhile, the expression of GSK3β was decreased. Of note, the expression of the Wnt/β-catenin pathway-related genes changed significantly in the context of TDM induction, as per RNA sequencing (RNA seq) data. In addition, the experiment showed that new dentin was visible in rat mandible cultured with TDM, and the thickness was significantly thicker than that of the control group. In addition, immunohistochemical staining showed lower GSK3β expression in new dentin. Consistently, the GSK3β knockdown hDPSCs performed enhanced odotogenesis compared with the control groups. However, GSK3β overexpressing could decrease odotogenesis of TDM-induced hDPSCs. These results were confirmed in immunodeficient mice and Wistar rats. These suggest that TDM promotes odontogenic differentiation of hDPSCs by directly targeting GSK3β and activating the canonical Wnt/β-catenin signaling pathway and provide a theoretical basis for tooth regeneration engineering. |
| first_indexed | 2024-04-24T08:14:42Z |
| format | Article |
| id | doaj.art-278270b37b3e4fa89d92696ac77baa23 |
| institution | Directory Open Access Journal |
| issn | 2452-199X |
| language | English |
| last_indexed | 2024-04-24T08:14:42Z |
| publishDate | 2022-01-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Bioactive Materials |
| spelling | doaj.art-278270b37b3e4fa89d92696ac77baa232024-04-17T04:17:15ZengKeAi Communications Co., Ltd.Bioactive Materials2452-199X2022-01-0178597Treated dentin matrix induces odontogenic differentiation of dental pulp stem cells via regulation of Wnt/β-catenin signalingSirui Liu0Jingjing Sun1Shuai Yuan2Yanyu Yang3Yuping Gong4Ying Wang5Runying Guo6Xue Zhang7Yiming Liu8Hongyan Mi9Meiyue Wang10Mengzhe Liu11Rui Li12Department of Stomatology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China; Academy of Medical Sciences at Zhengzhou University, Zhengzhou, ChinaDepartment of Stomatology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinaDepartment of Stomatology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinaCollege of Materials Science and Engineering, Zhengzhou University Zhengzhou, Henan 450001, ChinaDepartment of Stomatology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinaDepartment of Stomatology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinaDepartment of Stomatology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China; Academy of Medical Sciences at Zhengzhou University, Zhengzhou, ChinaDepartment of Stomatology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinaDepartment of Stomatology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinaDepartment of Stomatology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinaDepartment of Stomatology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinaDepartment of Stomatology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinaDepartment of Stomatology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China; Corresponding author.Treated dentin matrix (TDM) is an ideal scaffold material containing multiple extracellular matrix factors. The canonical Wnt signaling pathway is necessary for tooth regeneration. Thus, this study investigated whether the TDM can promote the odontogenic differentiation of human dental pulp stem cells (hDPSCs) and determined the potential role of Wnt/β-catenin signaling in this process. Different concentrations of TDM promoted the dental differentiation of the hDPSCs and meanwhile, the expression of GSK3β was decreased. Of note, the expression of the Wnt/β-catenin pathway-related genes changed significantly in the context of TDM induction, as per RNA sequencing (RNA seq) data. In addition, the experiment showed that new dentin was visible in rat mandible cultured with TDM, and the thickness was significantly thicker than that of the control group. In addition, immunohistochemical staining showed lower GSK3β expression in new dentin. Consistently, the GSK3β knockdown hDPSCs performed enhanced odotogenesis compared with the control groups. However, GSK3β overexpressing could decrease odotogenesis of TDM-induced hDPSCs. These results were confirmed in immunodeficient mice and Wistar rats. These suggest that TDM promotes odontogenic differentiation of hDPSCs by directly targeting GSK3β and activating the canonical Wnt/β-catenin signaling pathway and provide a theoretical basis for tooth regeneration engineering.http://www.sciencedirect.com/science/article/pii/S2452199X21002474Dental pulp stem cellsTreated dentin matrixGSK3βOdontogenic differentiationWnt/β-catenin signaling pathway |
| spellingShingle | Sirui Liu Jingjing Sun Shuai Yuan Yanyu Yang Yuping Gong Ying Wang Runying Guo Xue Zhang Yiming Liu Hongyan Mi Meiyue Wang Mengzhe Liu Rui Li Treated dentin matrix induces odontogenic differentiation of dental pulp stem cells via regulation of Wnt/β-catenin signaling Bioactive Materials Dental pulp stem cells Treated dentin matrix GSK3β Odontogenic differentiation Wnt/β-catenin signaling pathway |
| title | Treated dentin matrix induces odontogenic differentiation of dental pulp stem cells via regulation of Wnt/β-catenin signaling |
| title_full | Treated dentin matrix induces odontogenic differentiation of dental pulp stem cells via regulation of Wnt/β-catenin signaling |
| title_fullStr | Treated dentin matrix induces odontogenic differentiation of dental pulp stem cells via regulation of Wnt/β-catenin signaling |
| title_full_unstemmed | Treated dentin matrix induces odontogenic differentiation of dental pulp stem cells via regulation of Wnt/β-catenin signaling |
| title_short | Treated dentin matrix induces odontogenic differentiation of dental pulp stem cells via regulation of Wnt/β-catenin signaling |
| title_sort | treated dentin matrix induces odontogenic differentiation of dental pulp stem cells via regulation of wnt β catenin signaling |
| topic | Dental pulp stem cells Treated dentin matrix GSK3β Odontogenic differentiation Wnt/β-catenin signaling pathway |
| url | http://www.sciencedirect.com/science/article/pii/S2452199X21002474 |
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