Gravity filtration of environmental DNA: A simple, fast, and power-free method
Filtration is required during the collection of trace amounts of environmental DNA (eDNA) from water samples to achieve a concentration sufficient for downstream molecular experiments. To date, collected water samples have been filtered by humans or electric power using various instruments. We devel...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-01-01
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| Series: | MethodsX |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2215016122002187 |
| _version_ | 1811184629968797696 |
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| author | Shin-ichiro Oka Masaki Miya Tetsuya Sado |
| author_facet | Shin-ichiro Oka Masaki Miya Tetsuya Sado |
| author_sort | Shin-ichiro Oka |
| collection | DOAJ |
| description | Filtration is required during the collection of trace amounts of environmental DNA (eDNA) from water samples to achieve a concentration sufficient for downstream molecular experiments. To date, collected water samples have been filtered by humans or electric power using various instruments. We developed a simple gravity filtration system that does not need for an external force. The system comprises a plastic bag filled with a water sample (1 L), a filter cartridge, and a long plastic tube (e.g., 2 m). When hung at a height equal to the tube length, this filtration unit can enable power-free collection and concentration of eDNA at any remote location within a reasonable time (10–60 min). • A simple, rapid, power-free, practical filtration system for environmental DNA analysis is reported. • If there is a place to hang the filtration system, filtration can be performed anywhere. • The filtration speed increased when the system was hung higher. |
| first_indexed | 2024-04-11T13:15:35Z |
| format | Article |
| id | doaj.art-2783f1383f9940c0a49c17f34f1913f2 |
| institution | Directory Open Access Journal |
| issn | 2215-0161 |
| language | English |
| last_indexed | 2024-04-11T13:15:35Z |
| publishDate | 2022-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | MethodsX |
| spelling | doaj.art-2783f1383f9940c0a49c17f34f1913f22022-12-22T04:22:24ZengElsevierMethodsX2215-01612022-01-019101838Gravity filtration of environmental DNA: A simple, fast, and power-free methodShin-ichiro Oka0Masaki Miya1Tetsuya Sado2Okinawa Churashima Research Center, Okinawa, Japan; Corresponding author.Natural History Museum and Institute, Chiba, JapanNatural History Museum and Institute, Chiba, JapanFiltration is required during the collection of trace amounts of environmental DNA (eDNA) from water samples to achieve a concentration sufficient for downstream molecular experiments. To date, collected water samples have been filtered by humans or electric power using various instruments. We developed a simple gravity filtration system that does not need for an external force. The system comprises a plastic bag filled with a water sample (1 L), a filter cartridge, and a long plastic tube (e.g., 2 m). When hung at a height equal to the tube length, this filtration unit can enable power-free collection and concentration of eDNA at any remote location within a reasonable time (10–60 min). • A simple, rapid, power-free, practical filtration system for environmental DNA analysis is reported. • If there is a place to hang the filtration system, filtration can be performed anywhere. • The filtration speed increased when the system was hung higher.http://www.sciencedirect.com/science/article/pii/S2215016122002187Gravity filtration system for environmental DNA |
| spellingShingle | Shin-ichiro Oka Masaki Miya Tetsuya Sado Gravity filtration of environmental DNA: A simple, fast, and power-free method MethodsX Gravity filtration system for environmental DNA |
| title | Gravity filtration of environmental DNA: A simple, fast, and power-free method |
| title_full | Gravity filtration of environmental DNA: A simple, fast, and power-free method |
| title_fullStr | Gravity filtration of environmental DNA: A simple, fast, and power-free method |
| title_full_unstemmed | Gravity filtration of environmental DNA: A simple, fast, and power-free method |
| title_short | Gravity filtration of environmental DNA: A simple, fast, and power-free method |
| title_sort | gravity filtration of environmental dna a simple fast and power free method |
| topic | Gravity filtration system for environmental DNA |
| url | http://www.sciencedirect.com/science/article/pii/S2215016122002187 |
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