Correlation of DNA load, genotyping, and clinical phenotype of Mycoplasma pneumoniae infection in children
IntroductionThis study aimed to investigate the correlation between Mycoplasma pneumoniae (MP)-DNA load in the bronchoalveolar lavage fluid (BALF) of children with MP pneumonia (MPP) and its subtypes, relevant laboratory data, imaging, extrapulmonary complications in infected children, and its clini...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2024-04-01
|
| Series: | Frontiers in Pediatrics |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fped.2024.1369431/full |
| _version_ | 1797217019354939392 |
|---|---|
| author | Wei Wang Wei Wang Lu Wang Lu Wang Zhaoqing Yin Shujuan Zeng Guohua Yao Yuqiao Liu Yulian Fang Yulian Fang Cuian Ma Hualei Cui Hualei Cui |
| author_facet | Wei Wang Wei Wang Lu Wang Lu Wang Zhaoqing Yin Shujuan Zeng Guohua Yao Yuqiao Liu Yulian Fang Yulian Fang Cuian Ma Hualei Cui Hualei Cui |
| author_sort | Wei Wang |
| collection | DOAJ |
| description | IntroductionThis study aimed to investigate the correlation between Mycoplasma pneumoniae (MP)-DNA load in the bronchoalveolar lavage fluid (BALF) of children with MP pneumonia (MPP) and its subtypes, relevant laboratory data, imaging, extrapulmonary complications in infected children, and its clinical significance in evaluating the disease.MethodsChildren hospitalized with MPP at Tianjin Children's Hospital between December 2017 and December 2020 were selected for the study, excluding those with mixed viral, bacterial, and fungal infections. Children were divided into low- and high-load groups according to the MP DNA load in BALF using real-time quantitative fluorescence polymerase chain reaction (PCR). After a successful MP culture, positive specimens were subjected to PCR-Restriction fragment length polymorphism and Multiple-locus variable number tandem repeat analysis typing. Basic data, clinical information, laboratory data, and radiological results were collected from all children included in the study.ResultsThe PI-I type dominated the different load groups. Children in the low-load group had more wheezing and shortness of breath; however, children in the high-load group had a higher length of hospitalization, maximum fever temperature, higher chills/chilliness, incidence of abdominal pain, and higher C-reactive protein (CRP), procalcitonin (PCT) and aspartate aminotransferase (AST) levels. Children in the high-load group were more likely to have imaging changes such as pleural effusion, and the incidence of respiratory infections and extrapulmonary complications was higher than that of those in the low-load group. We applied Spearman's correlation analysis to clarify the relationship between MP DNA load and the clinical severity of MPP. We found that MP DNA load was positively correlated with length of hospitalization, maximum fever temperature, CRP, PCT, Interleukin-6 (IL-6), and AST levels, and negatively correlated with fever and cough durations, white blood cell count (WBC), and proportion of monocytes (MONO). The degree of correlation was as follows: length of hospitalization > IL-6 > cough duration > AST > fever duration > PCT > WBC > proportion of MONO > maximum fever temperature > CRP levels.ConclusionsMP DNA load was not correlated with MP typing but was significantly correlated with the children's clinical phenotype. Therefore, the MP DNA load helps in the early diagnosis of infection and can better predict disease regression. |
| first_indexed | 2024-04-24T11:55:12Z |
| format | Article |
| id | doaj.art-278773e9900c4e57abd59a0dd9028222 |
| institution | Directory Open Access Journal |
| issn | 2296-2360 |
| language | English |
| last_indexed | 2024-04-24T11:55:12Z |
| publishDate | 2024-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pediatrics |
| spelling | doaj.art-278773e9900c4e57abd59a0dd90282222024-04-09T04:45:31ZengFrontiers Media S.A.Frontiers in Pediatrics2296-23602024-04-011210.3389/fped.2024.13694311369431Correlation of DNA load, genotyping, and clinical phenotype of Mycoplasma pneumoniae infection in childrenWei Wang0Wei Wang1Lu Wang2Lu Wang3Zhaoqing Yin4Shujuan Zeng5Guohua Yao6Yuqiao Liu7Yulian Fang8Yulian Fang9Cuian Ma10Hualei Cui11Hualei Cui12Tianjin Pediatric Research Institute, Tianjin Children’s Hospital (Tianjin University Children’s Hospital), Tianjin, ChinaTianjin Key Laboratory of Birth Defects for Prevention and Treatment, Tianjin, ChinaTianjin Pediatric Research Institute, Tianjin Children’s Hospital (Tianjin University Children’s Hospital), Tianjin, ChinaTianjin Key Laboratory of Birth Defects for Prevention and Treatment, Tianjin, ChinaDivision of Neonatology, The People’s Hospital of Dehong Autonomous Prefecture, Mangshi, Yunnan, ChinaDivision of Neonatology, Longgang District Central Hospital of Shenzhen, Shenzhen, Guangdong, ChinaInfectious Diseases Section, Tianjin Children’s Hospital (Tianjin University Children’s Hospital), Tianjin, ChinaInfectious Diseases Section, Tianjin Children’s Hospital (Tianjin University Children’s Hospital), Tianjin, ChinaTianjin Pediatric Research Institute, Tianjin Children’s Hospital (Tianjin University Children’s Hospital), Tianjin, ChinaTianjin Key Laboratory of Birth Defects for Prevention and Treatment, Tianjin, ChinaInfectious Diseases Section, Tianjin Children’s Hospital (Tianjin University Children’s Hospital), Tianjin, ChinaTianjin Pediatric Research Institute, Tianjin Children’s Hospital (Tianjin University Children’s Hospital), Tianjin, ChinaTianjin Key Laboratory of Birth Defects for Prevention and Treatment, Tianjin, ChinaIntroductionThis study aimed to investigate the correlation between Mycoplasma pneumoniae (MP)-DNA load in the bronchoalveolar lavage fluid (BALF) of children with MP pneumonia (MPP) and its subtypes, relevant laboratory data, imaging, extrapulmonary complications in infected children, and its clinical significance in evaluating the disease.MethodsChildren hospitalized with MPP at Tianjin Children's Hospital between December 2017 and December 2020 were selected for the study, excluding those with mixed viral, bacterial, and fungal infections. Children were divided into low- and high-load groups according to the MP DNA load in BALF using real-time quantitative fluorescence polymerase chain reaction (PCR). After a successful MP culture, positive specimens were subjected to PCR-Restriction fragment length polymorphism and Multiple-locus variable number tandem repeat analysis typing. Basic data, clinical information, laboratory data, and radiological results were collected from all children included in the study.ResultsThe PI-I type dominated the different load groups. Children in the low-load group had more wheezing and shortness of breath; however, children in the high-load group had a higher length of hospitalization, maximum fever temperature, higher chills/chilliness, incidence of abdominal pain, and higher C-reactive protein (CRP), procalcitonin (PCT) and aspartate aminotransferase (AST) levels. Children in the high-load group were more likely to have imaging changes such as pleural effusion, and the incidence of respiratory infections and extrapulmonary complications was higher than that of those in the low-load group. We applied Spearman's correlation analysis to clarify the relationship between MP DNA load and the clinical severity of MPP. We found that MP DNA load was positively correlated with length of hospitalization, maximum fever temperature, CRP, PCT, Interleukin-6 (IL-6), and AST levels, and negatively correlated with fever and cough durations, white blood cell count (WBC), and proportion of monocytes (MONO). The degree of correlation was as follows: length of hospitalization > IL-6 > cough duration > AST > fever duration > PCT > WBC > proportion of MONO > maximum fever temperature > CRP levels.ConclusionsMP DNA load was not correlated with MP typing but was significantly correlated with the children's clinical phenotype. Therefore, the MP DNA load helps in the early diagnosis of infection and can better predict disease regression.https://www.frontiersin.org/articles/10.3389/fped.2024.1369431/fullmycoplasma pneumoniaechildrenDNA loadgenotypingclinical characteristics |
| spellingShingle | Wei Wang Wei Wang Lu Wang Lu Wang Zhaoqing Yin Shujuan Zeng Guohua Yao Yuqiao Liu Yulian Fang Yulian Fang Cuian Ma Hualei Cui Hualei Cui Correlation of DNA load, genotyping, and clinical phenotype of Mycoplasma pneumoniae infection in children Frontiers in Pediatrics mycoplasma pneumoniae children DNA load genotyping clinical characteristics |
| title | Correlation of DNA load, genotyping, and clinical phenotype of Mycoplasma pneumoniae infection in children |
| title_full | Correlation of DNA load, genotyping, and clinical phenotype of Mycoplasma pneumoniae infection in children |
| title_fullStr | Correlation of DNA load, genotyping, and clinical phenotype of Mycoplasma pneumoniae infection in children |
| title_full_unstemmed | Correlation of DNA load, genotyping, and clinical phenotype of Mycoplasma pneumoniae infection in children |
| title_short | Correlation of DNA load, genotyping, and clinical phenotype of Mycoplasma pneumoniae infection in children |
| title_sort | correlation of dna load genotyping and clinical phenotype of mycoplasma pneumoniae infection in children |
| topic | mycoplasma pneumoniae children DNA load genotyping clinical characteristics |
| url | https://www.frontiersin.org/articles/10.3389/fped.2024.1369431/full |
| work_keys_str_mv | AT weiwang correlationofdnaloadgenotypingandclinicalphenotypeofmycoplasmapneumoniaeinfectioninchildren AT weiwang correlationofdnaloadgenotypingandclinicalphenotypeofmycoplasmapneumoniaeinfectioninchildren AT luwang correlationofdnaloadgenotypingandclinicalphenotypeofmycoplasmapneumoniaeinfectioninchildren AT luwang correlationofdnaloadgenotypingandclinicalphenotypeofmycoplasmapneumoniaeinfectioninchildren AT zhaoqingyin correlationofdnaloadgenotypingandclinicalphenotypeofmycoplasmapneumoniaeinfectioninchildren AT shujuanzeng correlationofdnaloadgenotypingandclinicalphenotypeofmycoplasmapneumoniaeinfectioninchildren AT guohuayao correlationofdnaloadgenotypingandclinicalphenotypeofmycoplasmapneumoniaeinfectioninchildren AT yuqiaoliu correlationofdnaloadgenotypingandclinicalphenotypeofmycoplasmapneumoniaeinfectioninchildren AT yulianfang correlationofdnaloadgenotypingandclinicalphenotypeofmycoplasmapneumoniaeinfectioninchildren AT yulianfang correlationofdnaloadgenotypingandclinicalphenotypeofmycoplasmapneumoniaeinfectioninchildren AT cuianma correlationofdnaloadgenotypingandclinicalphenotypeofmycoplasmapneumoniaeinfectioninchildren AT hualeicui correlationofdnaloadgenotypingandclinicalphenotypeofmycoplasmapneumoniaeinfectioninchildren AT hualeicui correlationofdnaloadgenotypingandclinicalphenotypeofmycoplasmapneumoniaeinfectioninchildren |