Fruit Derived Potentially Bioactive Bioengineered Silver Nanoparticles
Abu Baker,1,* Sana Iram,2,* Asad Syed,3 Abdallah M Elgorban,3 Ali H Bahkali,3 Khurshid Ahmad,2 Mohd Sajid Khan,1 Jihoe Kim2 1Nanomedicine & Nanobiotechnology Lab, Department of Biosciences, Integral University, Lucknow, 226026, India; 2Department of Medical Biotechnology and Rese...
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| Format: | Article |
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Dove Medical Press
2021-11-01
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| Series: | International Journal of Nanomedicine |
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| Online Access: | https://www.dovepress.com/fruit-derived-potentially-bioactive-bioengineered-silver-nanoparticles-peer-reviewed-fulltext-article-IJN |
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| author | Baker A Iram S Syed A Elgorban AM Bahkali AH Ahmad K Khan Sajid M Kim J |
| author_facet | Baker A Iram S Syed A Elgorban AM Bahkali AH Ahmad K Khan Sajid M Kim J |
| author_sort | Baker A |
| collection | DOAJ |
| description | Abu Baker,1,* Sana Iram,2,* Asad Syed,3 Abdallah M Elgorban,3 Ali H Bahkali,3 Khurshid Ahmad,2 Mohd Sajid Khan,1 Jihoe Kim2 1Nanomedicine & Nanobiotechnology Lab, Department of Biosciences, Integral University, Lucknow, 226026, India; 2Department of Medical Biotechnology and Research Institute of Cell Culture, Yeungnam University, Gyeongsan, 38541, Republic of Korea; 3Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, 11451, Saudi Arabia*These authors contributed equally to this workCorrespondence: Mohd Sajid KhanDepartment of Biosciences, Integral University, Lucknow, UP, 226026, IndiaTel +522-2890812, 6451039; Tel +522-2890812, 6451039Fax +522-2890809Email research.sajid@gmail.comJihoe KimDepartment of Medical Biotechnology, Yeungnam University, Gyeongsan, 38541, South KoreaEmail kimjihoe@ynu.ac.krIntroduction: Protein-derived biogenic syntheses of inorganic nanoparticles have gained immense attention because of their broad spectrum of applications. Proteins offer a reducing environment to enable the synthesis of nanoparticles and encapsulate synthesized nanoparticles and provide them temporal stability in addition to biocompatibility.Methods: In the present study, Benincasa hispida fruit proteins were used to synthesize silver nanoparticles (AgNPs) at 37 °C over five days of incubation. The synthesis of AgNPs was confirmed by UV-Vis spectroscopy, TEM, zeta potential, and DLS analyses. Further, these NPs depicted antibacterial and antibiofilm effects. Additionally, the anticancer activities of nanoparticles were also tested against the lung cancer cell line (A549) with respect to the normal cell line (NRK) using MTT assay. Further, the estimation of ROS generation through DCFH-DA staining along with a reduction in mitochondrial membrane potential by Mito Tracker Red CMX staining was carried out. Moreover, nuclear degradation in the AgNPs treated cells was cross-checked by DAPI staining.Results: The average size of AgNPs was detected to be 27 ± 1 nm by TEM analysis, whereas surface encapsulation by protein was determined by FTIR spectroscopy. These NPs were effective against bacterial pathogens such as Escherichia coli, Staphylococcus aureus, Salmonella enteric, and Staphylococcus epidermis with MICs of 148.12 μg/mL, 165.63 μg/mL, 162.77 μg/mL, and 124.88 μg/mL, respectively. Furthermore, these nanoparticles inhibit the formation of biofilms of E. coli, S. aureus, S. enteric, and S. epidermis by 71.14%, 73.89%, 66.66%, and 64.81%, respectively. Similarly, these nanoparticles were also found to inhibit (IC50 = 57.11 μM) the lung cancer cell line (A549). At the same time, they were non-toxic against NRK cells up to a concentration of 200 μM.Discussion: We successfully synthesized potentially potent antibacterial, antibiofilm and anticancer biogenic AgNPs.Keywords: AgNPs, green synthesis, Benincasa hispida, antibacterial, antibiofilm, anticancer |
| first_indexed | 2024-12-17T21:08:11Z |
| format | Article |
| id | doaj.art-2787a339e78e4d3fb22ac57d1e9261e2 |
| institution | Directory Open Access Journal |
| issn | 1178-2013 |
| language | English |
| last_indexed | 2024-12-17T21:08:11Z |
| publishDate | 2021-11-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | International Journal of Nanomedicine |
| spelling | doaj.art-2787a339e78e4d3fb22ac57d1e9261e22022-12-21T21:32:31ZengDove Medical PressInternational Journal of Nanomedicine1178-20132021-11-01Volume 167711772670694Fruit Derived Potentially Bioactive Bioengineered Silver NanoparticlesBaker AIram SSyed AElgorban AMBahkali AHAhmad KKhan Sajid MKim JAbu Baker,1,* Sana Iram,2,* Asad Syed,3 Abdallah M Elgorban,3 Ali H Bahkali,3 Khurshid Ahmad,2 Mohd Sajid Khan,1 Jihoe Kim2 1Nanomedicine & Nanobiotechnology Lab, Department of Biosciences, Integral University, Lucknow, 226026, India; 2Department of Medical Biotechnology and Research Institute of Cell Culture, Yeungnam University, Gyeongsan, 38541, Republic of Korea; 3Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, 11451, Saudi Arabia*These authors contributed equally to this workCorrespondence: Mohd Sajid KhanDepartment of Biosciences, Integral University, Lucknow, UP, 226026, IndiaTel +522-2890812, 6451039; Tel +522-2890812, 6451039Fax +522-2890809Email research.sajid@gmail.comJihoe KimDepartment of Medical Biotechnology, Yeungnam University, Gyeongsan, 38541, South KoreaEmail kimjihoe@ynu.ac.krIntroduction: Protein-derived biogenic syntheses of inorganic nanoparticles have gained immense attention because of their broad spectrum of applications. Proteins offer a reducing environment to enable the synthesis of nanoparticles and encapsulate synthesized nanoparticles and provide them temporal stability in addition to biocompatibility.Methods: In the present study, Benincasa hispida fruit proteins were used to synthesize silver nanoparticles (AgNPs) at 37 °C over five days of incubation. The synthesis of AgNPs was confirmed by UV-Vis spectroscopy, TEM, zeta potential, and DLS analyses. Further, these NPs depicted antibacterial and antibiofilm effects. Additionally, the anticancer activities of nanoparticles were also tested against the lung cancer cell line (A549) with respect to the normal cell line (NRK) using MTT assay. Further, the estimation of ROS generation through DCFH-DA staining along with a reduction in mitochondrial membrane potential by Mito Tracker Red CMX staining was carried out. Moreover, nuclear degradation in the AgNPs treated cells was cross-checked by DAPI staining.Results: The average size of AgNPs was detected to be 27 ± 1 nm by TEM analysis, whereas surface encapsulation by protein was determined by FTIR spectroscopy. These NPs were effective against bacterial pathogens such as Escherichia coli, Staphylococcus aureus, Salmonella enteric, and Staphylococcus epidermis with MICs of 148.12 μg/mL, 165.63 μg/mL, 162.77 μg/mL, and 124.88 μg/mL, respectively. Furthermore, these nanoparticles inhibit the formation of biofilms of E. coli, S. aureus, S. enteric, and S. epidermis by 71.14%, 73.89%, 66.66%, and 64.81%, respectively. Similarly, these nanoparticles were also found to inhibit (IC50 = 57.11 μM) the lung cancer cell line (A549). At the same time, they were non-toxic against NRK cells up to a concentration of 200 μM.Discussion: We successfully synthesized potentially potent antibacterial, antibiofilm and anticancer biogenic AgNPs.Keywords: AgNPs, green synthesis, Benincasa hispida, antibacterial, antibiofilm, anticancerhttps://www.dovepress.com/fruit-derived-potentially-bioactive-bioengineered-silver-nanoparticles-peer-reviewed-fulltext-article-IJNagnpsgreen synthesisbenincasa hispidaantibacterialantibiofilmanticancer |
| spellingShingle | Baker A Iram S Syed A Elgorban AM Bahkali AH Ahmad K Khan Sajid M Kim J Fruit Derived Potentially Bioactive Bioengineered Silver Nanoparticles International Journal of Nanomedicine agnps green synthesis benincasa hispida antibacterial antibiofilm anticancer |
| title | Fruit Derived Potentially Bioactive Bioengineered Silver Nanoparticles |
| title_full | Fruit Derived Potentially Bioactive Bioengineered Silver Nanoparticles |
| title_fullStr | Fruit Derived Potentially Bioactive Bioengineered Silver Nanoparticles |
| title_full_unstemmed | Fruit Derived Potentially Bioactive Bioengineered Silver Nanoparticles |
| title_short | Fruit Derived Potentially Bioactive Bioengineered Silver Nanoparticles |
| title_sort | fruit derived potentially bioactive bioengineered silver nanoparticles |
| topic | agnps green synthesis benincasa hispida antibacterial antibiofilm anticancer |
| url | https://www.dovepress.com/fruit-derived-potentially-bioactive-bioengineered-silver-nanoparticles-peer-reviewed-fulltext-article-IJN |
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