A standard photomap of the ovarian nurse cell chromosomes for the dominant malaria vector in Europe and Middle East Anopheles sacharovi
Abstract Background Anopheles sacharovi is a dominant malaria vector species in South Europe and the Middle East which has a highly plastic behaviour at both adult and larval stages. Such plasticity has prevented this species from eradication by several anti-vector campaigns. The development of new...
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| Format: | Article |
| Language: | English |
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BMC
2018-07-01
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| Series: | Malaria Journal |
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| Online Access: | http://link.springer.com/article/10.1186/s12936-018-2428-9 |
| _version_ | 1818695025387634688 |
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| author | Gleb N. Artemov Alena I. Velichevskaya Semen M. Bondarenko Gayane H. Karagyan Sargis A. Aghayan Marine S. Arakelyan Vladimir N. Stegniy Igor V. Sharakhov Maria V. Sharakhova |
| author_facet | Gleb N. Artemov Alena I. Velichevskaya Semen M. Bondarenko Gayane H. Karagyan Sargis A. Aghayan Marine S. Arakelyan Vladimir N. Stegniy Igor V. Sharakhov Maria V. Sharakhova |
| author_sort | Gleb N. Artemov |
| collection | DOAJ |
| description | Abstract Background Anopheles sacharovi is a dominant malaria vector species in South Europe and the Middle East which has a highly plastic behaviour at both adult and larval stages. Such plasticity has prevented this species from eradication by several anti-vector campaigns. The development of new genome-based strategies for vector control will benefit from genome sequencing and physical chromosome mapping of this mosquito. Although a cytogenetic photomap for chromosomes from salivary glands of An. sacharovi has been developed, no cytogenetic map suitable for physical genome mapping is available. Methods Mosquitoes for this study were collected at adult stage in animal shelters in Armenia. Polytene chromosome preparations were prepared from ovarian nurse cells. Fluorescent in situ hybridization (FISH) was performed using PCR amplified probes. Results This study constructed a high-quality standard photomap for polytene chromosomes from ovarian nurse cells of An. sacharovi. Following the previous nomenclature, chromosomes were sub-divided into 39 numbered and 119 lettered sub-divisions. Chromosomal landmarks for the chromosome recognition were described. Using FISH, 4 PCR-amplified genic probes were mapped to the chromosomes. The positions of the probes demonstrated gene order reshuffling between An. sacharovi and Anopheles atroparvus which has not been seen cytologically. In addition, this study described specific chromosomal landmarks that can be used for the cytotaxonomic diagnostics of An. sacharovi based on the banding pattern of its polytene chromosomes. Conclusions This study constructed a high-quality standard photomap for ovarian nurse cell chromosomes of An. sacharovi and validated its utility for physical genome mapping. Based on the map, cytotaxonomic features for identification of An. sacharovi have been described. The cytogenetic map constructed in this study will assist in creating a chromosome-based genome assembly for this mosquito and in developing cytotaxonomic tools for identification of other species from the Maculipennis group. |
| first_indexed | 2024-12-17T13:38:55Z |
| format | Article |
| id | doaj.art-278c39ea3e2e4a96b7fdfba9804400e0 |
| institution | Directory Open Access Journal |
| issn | 1475-2875 |
| language | English |
| last_indexed | 2024-12-17T13:38:55Z |
| publishDate | 2018-07-01 |
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| series | Malaria Journal |
| spelling | doaj.art-278c39ea3e2e4a96b7fdfba9804400e02022-12-21T21:46:21ZengBMCMalaria Journal1475-28752018-07-0117111110.1186/s12936-018-2428-9A standard photomap of the ovarian nurse cell chromosomes for the dominant malaria vector in Europe and Middle East Anopheles sacharoviGleb N. Artemov0Alena I. Velichevskaya1Semen M. Bondarenko2Gayane H. Karagyan3Sargis A. Aghayan4Marine S. Arakelyan5Vladimir N. Stegniy6Igor V. Sharakhov7Maria V. Sharakhova8Laboratory of Ecology, Genetics and Environment Protection, Tomsk State UniversityLaboratory of Ecology, Genetics and Environment Protection, Tomsk State UniversityLaboratory of Ecology, Genetics and Environment Protection, Tomsk State UniversityScientific Center of Zoology and Hydroecology, The National Academy of Sciences of the Republic of ArmeniaScientific Center of Zoology and Hydroecology, The National Academy of Sciences of the Republic of ArmeniaChair of Zoology, Yerevan State UniversityLaboratory of Ecology, Genetics and Environment Protection, Tomsk State UniversityLaboratory of Ecology, Genetics and Environment Protection, Tomsk State UniversityLaboratory of Ecology, Genetics and Environment Protection, Tomsk State UniversityAbstract Background Anopheles sacharovi is a dominant malaria vector species in South Europe and the Middle East which has a highly plastic behaviour at both adult and larval stages. Such plasticity has prevented this species from eradication by several anti-vector campaigns. The development of new genome-based strategies for vector control will benefit from genome sequencing and physical chromosome mapping of this mosquito. Although a cytogenetic photomap for chromosomes from salivary glands of An. sacharovi has been developed, no cytogenetic map suitable for physical genome mapping is available. Methods Mosquitoes for this study were collected at adult stage in animal shelters in Armenia. Polytene chromosome preparations were prepared from ovarian nurse cells. Fluorescent in situ hybridization (FISH) was performed using PCR amplified probes. Results This study constructed a high-quality standard photomap for polytene chromosomes from ovarian nurse cells of An. sacharovi. Following the previous nomenclature, chromosomes were sub-divided into 39 numbered and 119 lettered sub-divisions. Chromosomal landmarks for the chromosome recognition were described. Using FISH, 4 PCR-amplified genic probes were mapped to the chromosomes. The positions of the probes demonstrated gene order reshuffling between An. sacharovi and Anopheles atroparvus which has not been seen cytologically. In addition, this study described specific chromosomal landmarks that can be used for the cytotaxonomic diagnostics of An. sacharovi based on the banding pattern of its polytene chromosomes. Conclusions This study constructed a high-quality standard photomap for ovarian nurse cell chromosomes of An. sacharovi and validated its utility for physical genome mapping. Based on the map, cytotaxonomic features for identification of An. sacharovi have been described. The cytogenetic map constructed in this study will assist in creating a chromosome-based genome assembly for this mosquito and in developing cytotaxonomic tools for identification of other species from the Maculipennis group.http://link.springer.com/article/10.1186/s12936-018-2428-9Anopheles sacharoviMosquitoGytogenetic mapFluorescence in situ hybridization |
| spellingShingle | Gleb N. Artemov Alena I. Velichevskaya Semen M. Bondarenko Gayane H. Karagyan Sargis A. Aghayan Marine S. Arakelyan Vladimir N. Stegniy Igor V. Sharakhov Maria V. Sharakhova A standard photomap of the ovarian nurse cell chromosomes for the dominant malaria vector in Europe and Middle East Anopheles sacharovi Malaria Journal Anopheles sacharovi Mosquito Gytogenetic map Fluorescence in situ hybridization |
| title | A standard photomap of the ovarian nurse cell chromosomes for the dominant malaria vector in Europe and Middle East Anopheles sacharovi |
| title_full | A standard photomap of the ovarian nurse cell chromosomes for the dominant malaria vector in Europe and Middle East Anopheles sacharovi |
| title_fullStr | A standard photomap of the ovarian nurse cell chromosomes for the dominant malaria vector in Europe and Middle East Anopheles sacharovi |
| title_full_unstemmed | A standard photomap of the ovarian nurse cell chromosomes for the dominant malaria vector in Europe and Middle East Anopheles sacharovi |
| title_short | A standard photomap of the ovarian nurse cell chromosomes for the dominant malaria vector in Europe and Middle East Anopheles sacharovi |
| title_sort | standard photomap of the ovarian nurse cell chromosomes for the dominant malaria vector in europe and middle east anopheles sacharovi |
| topic | Anopheles sacharovi Mosquito Gytogenetic map Fluorescence in situ hybridization |
| url | http://link.springer.com/article/10.1186/s12936-018-2428-9 |
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